NCF4 / P40PHOX Antibody

NCF4 / P40PHOX Polyclonal Antibody for Western blot, IHC

>> See 3 other antibodies for NCF4 (NCF4; P40PHOX; neutrophil cytosolic factor 4, 40kDa ; CTA-833B7.1; MGC3810; NCF; SH3PXD4; OTTHUMP00000028736; OTTHUMP00000043756; neutrophil NADPH oxidase factor 4; neutrophil cytosol factor 4; neutrophil cytosolic factor 4 (40kD))
Synonyms:
NCF4; P40PHOX; neutrophil cytosolic factor 4, 40kDa ; CTA-833B7.1; MGC3810; NCF; SH3PXD4; OTTHUMP00000028736; OTTHUMP00000043756; neutrophil NADPH oxidase factor 4; neutrophil cytosol factor 4; neutrophil cytosolic factor 4 (40kD)
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Goat
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu)
Immunogen: Synthetic peptide sequence (KDFPEEDDPTN) corresponding to the internal amino acids of NCF4
Ordering Information
Pierce NCF4 / P40PHOX Antibody
Product #
PA5-18751
Size
100 ug
Price
$316.00
Purchase
Add NCF4 / P40PHOX Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 0.3-1 ug/ml
Immunohistochemistry (IHC) 2-4µg/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 0.5mg/ml
Purification: Antigen affinity chromatography
Storage Buffer: TBS, pH 7.3, with 0.5% BSA
Preservative: 0.02% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
This antibody is predicted to react with canine based on sequence homology.
General Information
The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 to form a complex with neutrophil cytosolic factor 1 kinase, which suggests its role in PIkinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Product Images
  • NCF4 / P40PHOX Antibody (PA5-18751) in WB
    PA5-18751_WesternBlot_3.jpg

    Western Blot with anti-NCF4  /  P40PHOX Polyclonal Antibody (PA5-18751) (PA5-18751_WesternBlot_3.jpg)

    Western Blot with anti-NCF4 / P40PHOX Polyclonal Antibody (PA5-18751)

    Western blot of HEK293 overexpressing P40PHOX using PA5-18751, mock transfection as a control in first lane.
  • NCF4 / P40PHOX Antibody (PA5-18751) in WB
    PA5-18751_WesternBlot_2.jpg

    Western Blot with anti-NCF4  /  P40PHOX Polyclonal Antibody (PA5-18751) (PA5-18751_WesternBlot_2.jpg)

    Western Blot with anti-NCF4 / P40PHOX Polyclonal Antibody (PA5-18751)

    Western Blot staining of Daudi cell lysate using PA5-18751 at a concentration of 0.5 ug/ml, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
  • NCF4 / P40PHOX Antibody (PA5-18751) in IHC
    PA5-18751_Immunohistochemistry.jpg

    Immunohistochemistry with anti-NCF4  /  P40PHOX Polyclonal Antibody (PA5-18751) (PA5-18751_Immunohistochemistry.jpg)

    Immunohistochemistry with anti-NCF4 / P40PHOX Polyclonal Antibody (PA5-18751)

    Immunohistochemical staining of paraffin embedded of Human Tonsil using PA5-18751 at a concentration of 2.5 ug/ml. The tissue was processed by steamed antigen retrieval with citrate buffer pH 6 and stained with AP.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific