Myosin IIa Antibody

Myosin IIa Polyclonal Antibody for Western blot, IF, ICC

>> See 3 other antibodies for MYH9 (MYH9 variant protein; NMMHC II-a; NMMHC-A; NMMHC-IIA; cellular myosin heavy chain, type A; myosin heavy chain 9; myosin heavy chain, non-muscle IIa; myosin-9; non-muscle myosin heavy chain A; non-muscle myosin heavy chain IIa; non-muscle myosin heavy polypeptide 9; nonmuscle myosin heavy chain II-A)
Synonyms:
MYH9 variant protein; NMMHC II-a; NMMHC-A; NMMHC-IIA; cellular myosin heavy chain, type A; myosin heavy chain 9; myosin heavy chain, non-muscle IIa; myosin-9; non-muscle myosin heavy chain A; non-muscle myosin heavy chain IIa; non-muscle myosin heavy polypeptide 9; nonmuscle myosin heavy chain II-A
Entrez Gene ID:
Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt)
Immunogen: Synthetic peptide corresponding to the carboxy terminus of mouse myosin IIa.
Ordering Information
Pierce Myosin IIa Antibody
Product #
PA5-17025
Size
100 ul
Price
$250.00
Purchase
Add Myosin IIa Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:1000
Immunofluorescence (IF) 1:50
Immunocytochemistry (ICC) 1:50
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Purification: Affinity chromatography
Storage Buffer: 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol
Preservative: no preservative
Storage Conditions: -20°C
Product Specific Information
PA5-17025 targets Myosin IIa in ICC, and WB applications and shows reactivity with Human, mouse, and Rat samples.

The PA5-17025 immunogen is synthetic peptide corresponding to the carboxy terminus of mouse myosin IIa.

PA5-17025 detects Myosin IIa which has a predicted molecular weight of approximately 230 kDa.
General Information
This gene encodes a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain. The protein is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in MYH9 are the cause of non-syndromic sensorineural deafness autosomal dominant type 17, Epstein syndrome, Alport syndrome with macrothrombocytopenia, Sebastian syndrome, Fechtner syndrome and macrothrombocytopenia with progressive sensorineural deafness.
Product Images
  • Myosin IIa Antibody (PA5-17025) in WB
    PA5-17025_WesternBlot_2.jpg

    Western Blot with anti-Myosin IIa Polyclonal Antibody (PA5-17025) (PA5-17025_WesternBlot_2.jpg)

    Western Blot with anti-Myosin IIa Polyclonal Antibody (PA5-17025)

    Western blot analysis of Myosin IIa in extracts from COS cells, mock transfected or transfected with nonmuscle EGFP-myosin IIa or IIb using Myosin IIa polyclonal antibody (Product # PA5-17025).
  • Myosin IIa Antibody (PA5-17025) in WB
    PA5-17025_WesternBlot.jpg

    Western Blot with anti-Myosin IIa Polyclonal Antibody (PA5-17025) (PA5-17025_WesternBlot.jpg)

    Western Blot with anti-Myosin IIa Polyclonal Antibody (PA5-17025)

    Western blot analysis of Myosin IIa in extracts from Hela and HT-29 cells using Myosin IIa polyclonal antibody (Product # PA5-17025).
  • Myosin IIa Antibody (PA5-17025) in IF
    PA5-17025_Immunofluorescence_5.jpg

    Immunofluorescence with anti-Myosin IIa Polyclonal Antibody (PA5-17025) (PA5-17025_Immunofluorescence_5.jpg)

    Immunofluorescence with anti-Myosin IIa Polyclonal Antibody (PA5-17025)

    Immunofluorescent analysis of Myosin IIa in HeLa cells using a Myosin IIa polyclonal antibody (Product # PA5-17025) (green) showing colocalization with actin filaments that have been labeled with a fluorescent red phalloidin. DNA is labeled using a fluorescent blue dye.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific