Myc Epitope Tag Antibody (Myc.A7)

Myc Epitope Tag Monoclonal Antibody for Western blot, IF, IP, ELISA

Synonyms:
MRTL, bHLHe39, c-Myc
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: Myc.A7
Tested Species Reactivity: Tag (Tag)
Published Species Reactivity: Not Applicable (N/A)
Immunogen: Synthetic peptide: EQKLISEEDL (Myc).
Ordering Information
Pierce Myc Epitope Tag Antibody (Myc.A7)
Product #
MA1-21316
Size
100 µg
Price
$149.00
Purchase
Add Myc Epitope Tag Antibody (Myc.A7) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:1000 - 1:3000
Immunofluorescence (IF) 1:500 - 1:2000
Immunoprecipitation (IP) Assay dependent
ELISA (ELISA) Assay dependent
Published Applications Dilution
Western Blot (WB) See publications below
Immunocytochemistry (ICC) See publications below
Immunohistochemistry (IHC) See publications below
Immunoprecipitation (IP) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: Protein A
Storage Buffer: PBS, pH 7.2
Preservative: no preservative
Storage Conditions: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Specific Information
MA1-21316 detects N-terminal or C-terminal Myc tagged fusion proteins.

MA1-21316 has been successfully used in ELISA, immunofluorescence, immunoprecipitation and Western Blot procedures.

MA1-21316 immunogen is a synthetic peptide EQKLISEEDL (Myc).

MA1-21316 may be stored at 4ºC short term. Add 0.05% sodium azide if desired. For long term storage, store at -20ºC, avoiding freeze/thaw cycles.
General Information
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Antibodies against c-myc epitopes recognize overexpressed proteins containing Myc epitope tag fused to either amino- or carboxy-termini of targeted proteins.
Product Images
  • Myc Epitope Tag Antibody (MA1-21316) in WB
    MA1-21316_WesternBlot.jpg

    Western Blot with anti-Myc Epitope Tag Monoclonal Antibody [Myc.A7] (MA1-21316) (MA1-21316_WesternBlot.jpg)

    Western Blot with anti-Myc Epitope Tag Monoclonal Antibody [Myc.A7] (MA1-21316)

    1:1000 (1ug/mL) Ab dilution probed against HEK293 cells transfected with Myc-tagged protein vector; untransfected (1) and transfected (2)
Publications:
Western Blot
  Species / Dilution Summary
  N/A / 0
MA1-21316 was used in immunocytochemistry and western blot to study the role of GBF1 in the mechanism by which enterovirus 3A protein inhibits COP-I assembly

Dev Cell. 2006 Aug;11(2):191-201.
"A viral protein that blocks Arf1-mediated COP-I assembly by inhibiting the guanine nucleotide exchange factor GBF1."
Author(s): Wessels E, Duijsings D, Niu TK, Neumann S, Oorschot VM, de Lange F, Lanke KH, Klumperman J, Henke A, Jackson CL, Melchers WJ, van Kuppeveld FJ
Number of Citations: 35
(See PubMed article )
  N/A / 0
MA1-21316 was used in western blot to study the interaction between HSC20 and frataxin and its role in the biogenesis of iron-sulfur clusters and iron homeostasis

Hum Mol Genet. 2012 Apr 1;21(7):1457-69.
"HSC20 interacts with frataxin and is involved in iron-sulfur cluster biogenesis and iron homeostasis."
Author(s): Shan Y, Cortopassi G
Number of Citations: 3
(See PubMed article )
  N/A / 1:2000
MA1-21316 was used in immunohistochemistry and western blot to study the cellular pathology of a CHMP2B missense mutation associated with neurodegeneration

Biochem Biophys Res Commun. 2012 May 11;421(3):544-9.
"The functional analysis of the CHMP2B missense mutation associated with neurodegenerative diseases in the endo-lysosomal pathway."
Author(s): Han JH, Ryu HH, Jun MH, Jang DJ, Lee JA
Number of Citations: 2
(See PubMed article )
Immunocytochemistry
  Species / Dilution Summary
  N/A / 1:500
MA1-21316 was used in immunocytochemistry to explore the signaling pathways that regulate SMRT and N-CoR corepressors

J Biol Chem. 2004 Dec 24;279(52):54676-86.
"SMRT and N-CoR corepressors are regulated by distinct kinase signaling pathways."
Author(s): Jonas BA, Privalsky ML
Number of Citations: 27
(See PubMed article )
  N/A / 0
MA1-21316 was used in immunocytochemistry and western blot to study the role of GBF1 in the mechanism by which enterovirus 3A protein inhibits COP-I assembly

Dev Cell. 2006 Aug;11(2):191-201.
"A viral protein that blocks Arf1-mediated COP-I assembly by inhibiting the guanine nucleotide exchange factor GBF1."
Author(s): Wessels E, Duijsings D, Niu TK, Neumann S, Oorschot VM, de Lange F, Lanke KH, Klumperman J, Henke A, Jackson CL, Melchers WJ, van Kuppeveld FJ
Number of Citations: 35
(See PubMed article )
  N/A / 0
MA1-21316 was used in immunocytochemistry to evaluate the impact of stress-induced dissociation of polysomes on mRNA decay

Mol Cell Biol. 2006 Dec;26(23):8803-13.
"Polysome-bound endonuclease PMR1 is targeted to stress granules via stress-specific binding to TIA-1."
Author(s): Yang F, Peng Y, Murray EL, Otsuka Y, Kedersha N, Schoenberg DR
Number of Citations: 12
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  N/A / 1:200
MA1-21316 was used in immunohistochemistry and western blot to study the cellular pathology of a CHMP2B missense mutation associated with neurodegeneration

Biochem Biophys Res Commun. 2012 May 11;421(3):544-9.
"The functional analysis of the CHMP2B missense mutation associated with neurodegenerative diseases in the endo-lysosomal pathway."
Author(s): Han JH, Ryu HH, Jun MH, Jang DJ, Lee JA
Number of Citations: 2
(See PubMed article )
Immunoprecipitation
  Species / Dilution Summary
  N/A / 0
MA1-21316 was used in immunoprecipitation to study the interaction of the viral trans-frame P3N-PIPO protein with host PCaP1 and its role in potyvirus cell-to-cell movement

PLoS Pathog. 2012;8(4):e1002639.
"Interaction of the trans-frame potyvirus protein P3N-PIPO with host protein PCaP1 facilitates potyvirus movement."
Author(s): Vijayapalani P, Maeshima M, Nagasaki-Takekuchi N, Miller WA
Number of Citations: 6
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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