Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
100 µg of Protein A purified antibody (1 mg/ml) in 10 mM PBS, pH 7.2.
Western Blot (WB)
1:1000 - 1:3000
1:500 - 1:2000
Dot blot (DB)
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
MA1-21316 detects N-terminal or C-terminal Myc tagged fusion proteins.
MA1-21316 has been successfully used in ELISA, immunofluorescence, immunoprecipitation, Dot blot and Western Blot procedures.
MA1-21316 immunogen is a synthetic peptide EQKLISEEDL (Myc).
MA1-21316 may be stored at 4ºC short term. Add 0.05% sodium azide if desired. For long term storage, store at -20ºC, avoiding freeze/thaw cycles.
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Antibodies against c-myc epitopes recognize overexpressed proteins containing Myc epitope tag fused to either amino- or carboxy-termini of targeted proteins.
Myc Epitope Tag Antibody (MA1-21316) in WB
Western Blot with anti-Myc Epitope Tag Monoclonal Antibody [Myc.A7] (MA1-21316)