Mu-Calpain Antibody (9A4H8D3)

Mu-Calpain Monoclonal Antibody for Western blot, IF, ICC, IHC, IHC (F)

>> See 9 other antibodies for CAPN1 (CaNP, Calpain-I)
Synonyms:
CaNP, Calpain-I
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: 9A4H8D3
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt), Bovine (Bv), Hamster (Hm), Porcine (Po), Rabbit (Rb)
Published Species Reactivity: Arthropod (Ar), Bovine (Bv), Fish (Fs), Hamster (Hm), Human (Hu), Mouse (Ms), Porcine (Po), Rabbit (Rb), Rat (Rt), Yeast (Ys)
Immunogen: Purified bovine skeletal muscle 80 kDa mu-calpain subunit.
Ordering Information
Pierce Mu-Calpain Antibody (9A4H8D3)
Product #
MA3-940
Size
100 µl
Price
$285.00
Purchase
Add Mu-Calpain Antibody (9A4H8D3) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:2,000
Immunofluorescence (IF) 1:100
Immunocytochemistry (ICC) 1:100
Immunohistochemistry (IHC) 1:20
Immunohistochemistry (Frozen) (IHC (F)) Assay dependent
Published Applications Dilution
Western Blot (WB) See publications below
Immunocytochemistry (ICC) See publications below
Immunohistochemistry (IHC) See publications below
Immunoprecipitation (IP) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 4.1mg/ml
Storage Buffer: ascites
Preservative: 0.05% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
MA3-940 detects mu-calpain from human platelets and erythrocytes, bovine platelets, heart and skeletal muscle, rat myoblasts, kidney, liver and spleen, mouse lung, pig cultured cells and hamster and rabbit samples. This antibody does not cross-react with m-calpain, n-calpain, calmodulin or calpastatin.

MA3-940 has been successfully used in Western blot, immunofluorescence, immunohistochemistry, and immunocytochemistry procedures. By Western blot, this antibody detects an 80 kDa protein representing mu-calpain from human platelets and erythrocytes. Immunocytochemical staining of mu-calpain in LLC-PK1 cells with MA3-940 results in diffuse cytoplasmic staining. This product has not been shown to be effective in immunoprecipitation experiments.

The MA3-940 antigen is purified bovine skeletal muscle 80 kDa mu-calpain subunit. This antibody recognizes an epitope between amino acids 465-520 (domain III) of human mu-calpain.

General Information
The calpain (calcium-dependent protease or calcium-activated neutral protease) system consists of two ubiquitous forms of calpain (mu-calpain and m-calpain), a tissue specific calpain (n-calpain), and a calpain inhibitory protein (calpastatin). The calpain system has been detected in every vertebrate tissue examined, and has been suggested to play a regulatory role in cellular protein metabolism. This regulatory role may have important implications in platelet aggregation and pathologies associated with altered calcium homeostasis and protein metabolism such as ischemic cell injury and degenerative diseases. Inhibitors of calpain have been shown to block dexamethasone and low-level irradiation induced apoptosis in thymocytes suggesting that calpain has a regulatory or mechanistic role in apoptotic cell death.

Mu-Calpain, also known as Calpain-I, and m-calpain, also known as Calpain-II, are intracellular, calcium-dependent cysteine proteases.

Mu- and m-calpains are heterodimers consisting of 28 kDa and 80 kDa subunits. The 28 kDa subunit is identical in the two isoforms, but the 80 kDa subunits differ with ~50% sequence similarity. 28 kDa/80 kDa complexes are thought to be inactive proenzymes which, upon binding of calcium, undergo conformational changes that promotes cleavage of the 28 kDa subunit and results in enzyme activation.
Product Images
  • Mu-Calpain Antibody (MA3-940) in WB
    MA3-940_1_07232010.jpg

    Western Blot with anti-Mu-Calpain Monoclonal Antibody [9A4H8D3] (MA3-940) (MA3-940_1_07232010.jpg)

    Western Blot with anti-Mu-Calpain Monoclonal Antibody [9A4H8D3] (MA3-940)

    Western blot of mu-calpain in mouse lung extract using MA3-940.
  • Mu-Calpain Antibody (MA3-940) in IF
    MA3-940_IF_Hela.jpg

    Immunofluorescence with anti-Mu-Calpain Monoclonal Antibody [9A4H8D3] (MA3-940) (MA3-940_IF_Hela.jpg)

    Immunofluorescence with anti-Mu-Calpain Monoclonal Antibody [9A4H8D3] (MA3-940)

    Immunofluorescent analysis of Mu-Calpain in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Mu-Calpain monoclonal antibody (Product # MA3-940) at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Mu-Calpain staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
  • Mu-Calpain Antibody (MA3-940) in IF
    MA3-940_IF_U251.jpg

    Immunofluorescence with anti-Mu-Calpain Monoclonal Antibody [9A4H8D3] (MA3-940) (MA3-940_IF_U251.jpg)

    Immunofluorescence with anti-Mu-Calpain Monoclonal Antibody [9A4H8D3] (MA3-940)

    Immunofluorescent analysis of Mu-Calpain in U251 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Mu-Calpain monoclonal antibody (Product # MA3-940) at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Mu-Calpain staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
  • Mu-Calpain Antibody (MA3-940) in IF
    MA3-940_IF_MCF-7.jpg

    Immunofluorescence with anti-Mu-Calpain Monoclonal Antibody [9A4H8D3] (MA3-940) (MA3-940_IF_MCF-7.jpg)

    Immunofluorescence with anti-Mu-Calpain Monoclonal Antibody [9A4H8D3] (MA3-940)

    Immunofluorescent analysis of Mu-Calpain in MCF-7 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Mu-Calpain monoclonal antibody (Product # MA3-940) at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Mu-Calpain staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
  • Mu-Calpain Antibody (MA3-940) in IHC
    MA3-940_IHC_Human-Kidney tissue.jpg

    Immunohistochemistry with anti-Mu-Calpain Monoclonal Antibody [9A4H8D3] (MA3-940) (MA3-940_IHC_Human-Kidney tissue.jpg)

    Immunohistochemistry with anti-Mu-Calpain Monoclonal Antibody [9A4H8D3] (MA3-940)

    Immunohistochemistry was performed on normal biopsies of deparaffinized human kidney tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a Mouse Monoclonal Antibody recognizing Mu-Calpain (MA3-940 ) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  • Mu-Calpain Antibody (MA3-940) in IHC
    MA3-940_IHC_Human-Tonsil tissue.jpg

    Immunohistochemistry with anti-Mu-Calpain Monoclonal Antibody [9A4H8D3] (MA3-940) (MA3-940_IHC_Human-Tonsil tissue.jpg)

    Immunohistochemistry with anti-Mu-Calpain Monoclonal Antibody [9A4H8D3] (MA3-940)

    Immunohistochemistry was performed on normal biopsies of deparaffinized human tonsil tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a Mouse Monoclonal Antibody recognizing Mu-Calpain (MA3-940 ) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Publications:
Western Blot
  Species / Dilution Summary
  Ar / 1:4000
MA3-940 was used in western blot to study the involvement of protease activity in storage of red swamp crayfish

J Agric Food Chem. 2008 Sep 24;56(18):8658-63.
"Protease activity in post-mortem red swamp crayfish (Procambarus clarkii) muscle stored in modified atmosphere packaging."
Author(s): Chen G, Guttmann RP, Xiong YL, Webster CD, Romaire RP
Number of Citations: 1
(See PubMed article )
  Bv / 1:40-100
MA3-940 was used in western blot to study the effect of specific calpain antibodies for the regulation of calpain function

J Biol Chem. 1993 Dec 5;268(34):25740-7.
"Effect of monoclonal antibodies specific for the 28-kDa subunit on catalytic properties of the calpains."
Author(s): Cong J, Thompson VF, Goll DE
Number of Citations: 0
(See PubMed article )
  Bv / 1:10
MA3-940 was used in western blot to detect the changes of micro-calpain, m-calpain, and calpastatin in bovine muscle during postmortem storage

J Anim Sci. 1998 Sep;76(9):2415-34.
"Changes in the calpains and calpastatin during postmortem storage of bovine muscle."
Author(s): Boehm ML, Kendall TL, Thompson VF, Goll DE
Number of Citations: 0
(See PubMed article )
  Bv / 1:10,000
MA3-940 was used in western blot to investigate the role of inactivation of mu-calpain for tenderization of beef steaks during oxidative environments

J Anim Sci. 2004 Nov;82(11):3254-66.
"Oxidative environments decrease tenderization of beef steaks through inactivation of mu-calpain."
Author(s): Rowe LJ, Maddock KR, Lonergan SM, Huff-Lonergan E
Number of Citations: 0
(See PubMed article )
  Bv / Not Cited
MA3-940 was used in western blot to evaluate the effect of the postmortem storage time and temperature on calpain activity in bovine muscles

J Anim Sci. 2007 Oct;85(10):2670-81.
"Effect of postmortem storage on activity of mu- and m-calpain in five bovine muscles."
Author(s): Camou JP, Marchello JA, Thompson VF, Mares SW, Goll DE
Number of Citations: 1
(See PubMed article )
  Bv / 1:10,000
MA3-940 was used in western blot to isolate and characterize calpain isoforms and calpastatin

J Anim Sci. 2007 Dec;85(12):3400-14.
"Isolation and characterization of mu-calpain, m-calpain, and calpastatin from postmortem muscle. I. Initial steps."
Author(s): Camou JP, Mares SW, Marchello JA, Vazquez R, Taylor M, Thompson VF, Goll DE
Number of Citations: 1
(See PubMed article )
  Bv / 0
MA3-940 was used in western blot to investigate the effect of the high-oxygen modified atmosphere packaging system on oxidation and polymerization

Meat Sci. 2010 Aug;85(4):759-67.
"High-oxygen modified atmosphere packaging system induces lipid and myoglobin oxidation and protein polymerization."
Author(s): Kim YH, Huff-Lonergan E, Sebranek JG, Lonergan SM
Number of Citations: 1
(See PubMed article )
  Bv / 1:10000
MA3-940 was used in western blot to investigate the effect of protein denaturation on u-calpain activation

Meat Sci. 2010 Nov;86(3):883-7.
"Protein denaturing conditions in beef deep semimembranosus muscle results in limited ?-calpain activation and protein degradation."
Author(s): Kim YH, Lonergan SM, Huff-Lonergan E
Number of Citations: 1
(See PubMed article )
  Bv / 1:10000
MA3-940 was used in western blot to study the effects on beef quality of electrical input, wrapping, pre rigor temperature and different post rigor chilling rates

Meat Sci. 2012 May;91(1):62-8.
"High pre rigor temperature limits the ageing potential of beef that is not completely overcome by electrical stimulation and muscle restraining."
Author(s): Kim YH, Stuart A, Nygaard G, Rosenvold K
Number of Citations: 0
(See PubMed article )
  Bv / Not Cited
MA3-940 was used in western blot to study the biochemical factors governing commercially important beef cut tenderness

Meat Sci. 2012 Jul;91(3):247-54.
"Profile of biochemical traits influencing tenderness of muscles from the beef round."
Author(s): Anderson MJ, Lonergan SM, Fedler CA, Prusa KJ, Binning JM, Huff-Lonergan E
Number of Citations: 0
(See PubMed article )
  Bv / 1:5000
MA3-940 was used in immunoprecipitation and western blot to study whether postmortem low voltage stimulation of bovine muscle has any effect on meat tenderness and quality

Meat Sci. 2013 Jul;94(3):289-96.
"Effect of low voltage electrical stimulation on protein and quality changes in bovine muscles during postmortem aging."
Author(s): Kim YH, Lonergan SM, Grubbs JK, Cruzen SM, Fritchen AN, della Malva A, Marino R, Huff-Lonergan E
Number of Citations: 2
(See PubMed article )
  Fs / 0
MA3-940 was used in western blot to purify and characterize the calpain and calpastatin from rainbow trout

Comp Biochem Physiol B Biochem Mol Biol. 2007 Apr;146(4):445-55.
"Purification and characterization of calpain and calpastatin from rainbow trout, Oncorhynchus mykiss."
Author(s): Saito M, Li H, Thompson VF, Kunisaki N, Goll DE
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
MA3-940 was used in western blot to study the calpains present in human placenta

Life Sci. 2002 Apr 21;70(21):2493-508.
"The calpain system in human placenta."
Author(s): Thompson VF, Saldaña S, Cong J, Luedke DM, Goll DE
Number of Citations: 2
(See PubMed article )
  Hu / 1:2000
MA3-940 was used in western blot to study the role of mu-calpain in decidua from patients with recurrent miscarriage

Am J Reprod Immunol. 2008 Apr;59(4):339-46.
"Role of mu-calpain in human decidua for recurrent miscarriage."
Author(s): Kumagai K, Ozaki Y, Nakanishi T, Inomata M, Furuno T, Nakanishi M, Ogasawara MS
Number of Citations: 1
(See PubMed article )
  Hu / 0
MA3-940 was used in western blot to investigate the role of Arl13b in ciliary protein transport in C. elegans

J Cell Biol. 2010 Mar 22;188(6):953-69.
"Joubert syndrome Arl13b functions at ciliary membranes and stabilizes protein transport in Caenorhabditis elegans."
Author(s): Cevik S, Hori Y, Kaplan OI, Kida K, Toivenon T, Foley-Fisher C, Cottell D, Katada T, Kontani K, Blacque OE
Number of Citations: 1
(See PubMed article )
  Ms / 1:500
MA3-940 was used in western blot to investigate the role of calpain 2 in the ionomycin-induced cell death in mouse lens epithelial cells

Curr Eye Res. 2011 Oct;36(10):930-6.
"Involvement of calpain 2 in ionomycin-induced cell death in cultured mouse lens epithelial cells."
Author(s): Nakajima T, Shearer TR, Azuma M
Number of Citations: 1
(See PubMed article )
  Ms / 0
MA3-940 was used in western blot to study the effect of pH changes on breakdown of porcine cytoskeletal proteins

Meat Sci. 2007 Jun;76(2):359-65.
"Rate and extent of pH decline affect proteolysis of cytoskeletal proteins and water-holding capacity in pork."
Author(s): Bee G, Anderson AL, Lonergan SM, Huff-Lonergan E
Number of Citations: 1
(See PubMed article )
  Po / Not Cited
MA3-940 was used in western blot to study the effect of pH and ionic strength on mu- and m-calpain activity and the ability of calpastatin to inhibit the activity of mu- or m-calpain

J Anim Sci. 2005 Jun;83(6):1370-6.
"Effect of pH and ionic strength on mu- and m-calpain inhibition by calpastatin."
Author(s): Maddock KR, Huff-Lonergan E, Rowe LJ, Lonergan SM
Number of Citations: 0
(See PubMed article )
  Po / 1:10000
MA3-940 was used in western blot to investigate the postmortem changes of integrin, desmin, and calpain in pork

Meat Sci. 2006 Nov;74(3):578-85.
"Contribution of postmortem changes of integrin, desmin and ?-calpain to variation in water holding capacity of pork."
Author(s): Zhang WG, Lonergan SM, Gardner MA, Huff-Lonergan E
Number of Citations: 1
(See PubMed article )
  Ys / 1:2,000
MA3-940 was used in western blot to investigate the role of calpain during hereditary cataract formation in sheep

Invest Ophthalmol Vis Sci. 2005 Dec;46(12):4634-40.
"Calpain may contribute to hereditary cataract formation in sheep."
Author(s): Robertson LJ, Morton JD, Yamaguchi M, Bickerstaffe R, Shearer TR, Azuma M
Number of Citations: 1
(See PubMed article )
Immunocytochemistry
  Species / Dilution Summary
  Hm / 1:100
MA3-940 was used in immunocytochemistry to show that a calcium-binding ER protein with protective functions against calcium-induced apoptosis is a substrate for a calcium-activated protease

J Biol Chem. 1999 Oct 1;274(40):28476-83.
"The endoplasmic reticulum chaperone glycoprotein GRP94 with Ca(2+)-binding and antiapoptotic properties is a novel proteolytic target of calpain during etoposide-induced apoptosis."
Author(s): Reddy RK, Lu J, Lee AS
Number of Citations: 14
(See PubMed article )
  Hu / 1 mg/ml
MA3-940 was used in immunocytochemistry to compare the cellular localization of calpains and calpastatin in human A431 cells

Exp Cell Res. 1992 Nov;203(1):5-16.
"A comparison of the intracellular distribution of mu-calpain, m-calpain, and calpastatin in proliferating human A431 cells."
Author(s): Lane RD, Allan DM, Mellgren RL
Number of Citations: 12
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  Hu / 0
Ms / 0
MA3-940 was used in immunohistochemistry and immunohistochemistry to investigate the role of acid-sensing ion channel 1 in axonal injury and demyelination

Brain. 2011 Feb;134(Pt 2):571-84.
"Acid-sensing ion channel 1 is involved in both axonal injury and demyelination in multiple sclerosis and its animal model."
Author(s): Vergo S, Craner MJ, Etzensperger R, Attfield K, Friese MA, Newcombe J, Esiri M, Fugger L
Number of Citations: 1
(See PubMed article )
  Po / 1:100
MA3-940 was used in immunohistochemistry to investigate the effect of early postmortem biochemical factors on tenderness and water-holding capacity of three porcine muscles

J Anim Sci. 2004 Apr;82(4):1195-205.
"Early postmortem biochemical factors influence tenderness and water-holding capacity of three porcine muscles."
Author(s): Melody JL, Lonergan SM, Rowe LJ, Huiatt TW, Mayes MS, Huff-Lonergan E
Number of Citations: 1
(See PubMed article )
  Rb / Not Cited
MA3-940 was used in immunohistochemistry to study the importance of two major proteolytic systems in tranforming rabbit and rat muscles

Am J Physiol Cell Physiol. 2001 Feb;280(2):C239-47.
"Fiber type-specific expression of major proteolytic systems in fast- to slow-transforming rabbit muscle."
Author(s): Sultan KR, Dittrich BT, Leisner E, Paul N, Pette D
Number of Citations: 2
(See PubMed article )
Immunoprecipitation
  Species / Dilution Summary
  Rt / Not Cited
MA3-940 was used in immunoprecipitation and western blot to study whether postmortem low voltage stimulation of bovine muscle has any effect on meat tenderness and quality

Meat Sci. 2013 Jul;94(3):289-96.
"Effect of low voltage electrical stimulation on protein and quality changes in bovine muscles during postmortem aging."
Author(s): Kim YH, Lonergan SM, Grubbs JK, Cruzen SM, Fritchen AN, della Malva A, Marino R, Huff-Lonergan E
Number of Citations: 2
(See PubMed article )
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