Mouse anti-human IgG Secondary Antibody

human IgG Monoclonal Secondary Antibody for Western blot

Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Secondary Antibody
Clone: 4D2D9G8
Label: Unconjugated
Species Reactivity: Human (Hu)
Immunogen: Human IgG was isolated from human sera and purified by chromatography.
Ordering Information
Pierce Mouse anti-human IgG Secondary Antibody
Product #
MA5-14755
Size
100 ug
Price
$299.00
Purchase
Add Mouse anti-human IgG Secondary Antibody to your cart.
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form: 100 ug of purified antibody in PBS containing 0.03% sodium azide.


Applications Dilution *
Western Blot (WB) 1/500 - 1/2000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
MA5-14755 targets human IgG in WB applications and shows reactivity with Human samples.

The MA5-14755 immunogen is human IgG was isolated from human sera and purified by chromatography.

MA5-14755 detects human IgG which has a predicted molecular weight of approximately 50kDa.
General Information
Thermo Scientific Pierce Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
Product Images
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  • human IgG Secondary Antibody (MA5-14755) in WB
    Western Blot with anti-human IgG Monoclonal Secondary Antibody [4D2D9G8] (MA5-14755)

    Western Blot with anti-human IgG Monoclonal Secondary Antibody [4D2D9G8] (MA5-14755)

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    Western blot analysis using IgG antibody (lane 1, 2) and Albumin antibody (lane 3, 4) against human serum (lane 1, 3) and plasma (lane 2, 4).
     
 
 
References:
Roitt,I, et al "Immunology", Mosby, London, England, (1996) fourth edition.
Vlug, A. et al "The structure and function of human IgG subclasses". Eur.Clin.Lab. 8, 26 (1989).
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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