Mouse anti-Rat IgG (Kappa light chain) Secondary Antibody

Rat IgG (Kappa light chain) Monoclonal Secondary Antibody for FACS

Host / Isotype: Mouse / IgG2a
Class: Monoclonal
Type: Secondary Antibody
Clone: OX-12
Label: Unconjugated
Tested Species Reactivity: Rat (Rt)
Immunogen: Pooled rat serum IgG.
Ordering Information
Pierce Mouse anti-Rat IgG (Kappa light chain) Secondary Antibody
Product #
250 ug
Tested Applications Dilution *
Flow Cytometry (FACS) 1 µg/1 million cells
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: Protein G
Storage Buffer: PBS
Preservative: 0.02% sodium azide
Storage Conditions: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Specific Information
SA1-70012 detects rat Ig (kappa chain).

SA1-70012 has been successfully used in flow cytometry procedures.

The SA1-70012 immunogen is pooled rat serum IgG.
General Information
Thermo Scientific Pierce Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
Product Images
  • Rat IgG (Kappa light chain) Secondary Antibody (SA1-70012) in FACS

    Flow Cytometry with anti-Rat IgG (Kappa light chain) Monoclonal Secondary Antibody [OX-12] (SA1-70012) (Rat-IgG-Kappa-light-chain-SA1-70012-Antibody-Flowcytometry.jpg)

    Flow Cytometry with anti-Rat IgG (Kappa light chain) Monoclonal Secondary Antibody [OX-12] (SA1-70012)

    Flow cytometric analysis of spleen cells stained using a rat Ig, Kappa secondary antibody (Product # SA1-70012) at a 0.06 ug/10^6 cells dilution. The isotype control was a mouse IgG2a
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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