Mouse anti-Human IgG Fab Secondary Antibody

Human IgG Fab Monoclonal Secondary Antibody for Western blot, FACS, ELISA

Host / Isotype: Mouse / IgG2b
Class: Monoclonal
Type: Secondary Antibody
Clone: 4A11
Label: Unconjugated
Tested Species Reactivity: Human (Hu)
Immunogen: Purified human IgG
Ordering Information
Pierce Mouse anti-Human IgG Fab Secondary Antibody
Product #
100 µg
Tested Applications Dilution *
Western Blot (WB) Assay Dependent
Flow Cytometry (FACS) Assay Dependent
ELISA (ELISA) Assay Dependent
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: Ion-exchange chromatography
Storage Buffer: PBS, pH 7.4
Preservative: 15mM sodium azide
Storage Conditions: 4° C, do not freeze
Product Specific Information
General Information
Thermo Scientific Pierce Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
Product Images
  • Human IgG Fab Secondary Antibody (SA1-19255) in WB

    Western Blot with anti-Human IgG Fab Monoclonal Secondary Antibody [4A11] (SA1-19255) (Human-IgG-Fab-2-SA1-19255-Antibody-WB-20130709140326.jpg)

    Western Blot with anti-Human IgG Fab Monoclonal Secondary Antibody [4A11] (SA1-19255)

    Western Blotting analysis of human IgG Fab fragment using anti-human IgG Fab fragment secondary antibody (SA1-19255). Lane 1: Normal human immunoglobulins Lane 2: Normal bovine immunoglobulins.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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