* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
PBS, pH 7.4
15mM sodium azide
4° C, do not freeze
Product Specific Information
SA1-19255 detects human IgG, Fab fragment in human samples.
SA1-19255 has been successfully used in FACS, Western blot and ELISA procedures.
The SA1-19255 immunogen is purified human IgG.
Thermo Scientific Pierce Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
Human IgG Fab Secondary Antibody (SA1-19255) in WB
Western Blot with anti-Human IgG Fab Monoclonal Secondary Antibody [4A11] (SA1-19255)