Mouse anti-Human IgE Secondary Antibody

Human IgE Monoclonal Secondary Antibody for FACS, ELISA

Host / Isotype: Mouse / IgG2a
Class: Monoclonal
Type: Secondary Antibody
Clone: E411 (5H2)
Label: Unconjugated
Tested Species Reactivity: Human (Hu)
Immunogen: Immunoglobulin E from human serum.
Ordering Information
Pierce Mouse anti-Human IgE Secondary Antibody
Product #
1 mg
Tested Applications Dilution *
Flow Cytometry (FACS) Assay Dependent
ELISA (ELISA) Assay Dependent
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: Protein G
Storage Buffer: PBS, pH 7.2
Preservative: 0.09% sodium azide
Storage Conditions: 4°C or -20°C if preferred
Product Specific Information
General Information
Thermo Scientific Pierce Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
Product Images
  • Human IgE Secondary Antibody (MA1-82854) in ELISA

    ELISA with anti-Human IgE Monoclonal Secondary Antibody [E411 (5H2)] (MA1-82854) (Human-IgE_MA1-82854_Antibody_ELISA1.jpg)

    ELISA with anti-Human IgE Monoclonal Secondary Antibody [E411 (5H2)] (MA1-82854)

    ELISA analysis with a human IgE monoclonal antibody (Product # MA1-82854)) as the capture antibody, human IgE (RP-86985) as the antigen, and a human IgE monoclonal antibody (Product # MA1-82854) (conjugated to HRP) as the detection antibody
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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