100 µg of FITC conjugated antibody (0.1 mg/ml) in PBS with 15 mM sodium azide and 0.2% (w/v) high grade protease free BSA.
Flow Cytometry (FACS)
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
SA1-19599 detects human IgE in human samples.
SA1-19599 has been successfully used in FACS procedures.
The SA1-19599 immunogen is purified human IgE.
Thermo Scientific Pierce Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.