100 µg of antibody (1 mg/ml) in TBS with 15 mM sodium azide pH 8.0.
Flow Cytometry (FACS)
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
SA1-19259 detects human IgE in human samples.
SA1-19259 has been successfully used in FACS and ELISA procedures.
The SA1-19259 immunogen is purified human IgE.
Store at 2-8ºC. For long term storage, aliquot and store at -20ºC, avoiding freeze/thaw cycles.
Thermo Scientific Pierce Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
Human IgE Secondary Antibody (SA1-19259) in FACS
Flow Cytometry with anti-Human IgE Monoclonal Secondary Antibody [BE5] (SA1-19259)