500 ul of antibody purified from the ascites fluid by Protein G chromatography (200 ug/ml). Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.
Applications
Dilution *
Immunohistochemistry (Paraffin) (IHC (P))
1:25
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
MA5-11208 targets Human IgA (alpha-Heavy Chain) in IHC (P) applications and shows reactivity with Human samples.
The MA5-11208 immunogen is purified human alpha heavy chain.
General Information
Thermo Scientific Pierce Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
Product Images
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Human IgA (alpha-Heavy Chain) Secondary Antibody (MA5-11208) in IHC
Immunohistochemistry with anti-Human IgA (alpha-Heavy Chain) Monoclonal Secondary Antibody [GA01] (MA5-11208)
Formalin-fixed, paraffin-embedded human tonsil stained with IgA antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of IgA secreting cells.