Mint1 Antibody

Mint1 Polyclonal Antibody for Western blot

>> See 3 other antibodies for Apba1 (Munc-18 Interacting Protein 1, Amyloid beta A4 precursor protein-binding family A member 1, Neuron-specific X11 protein, Neuronal Munc18-1-interacting)
Synonyms:
Munc-18 Interacting Protein 1, Amyloid beta A4 precursor protein-binding family A member 1, Neuron-specific X11 protein, Neuronal Munc18-1-interacting
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Mouse (Ms), Rat (Rt)
Published Species Reactivity: Mouse (Ms), Rat (Rt)
Immunogen: Synthetic peptide corresponding to residues M(1) N H L E G S A E V E V A D E A P(17) C of rat Mint1.
Ordering Information
Pierce Mint1 Antibody
Product #
PA1-070
Size
100 µg
Price
$316.00
Purchase
Add Mint1 Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 4 µg/ml
Published Applications Dilution
Immunohistochemistry (IHC) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: Antigen affinity chromatography
Storage Buffer: PBS with 1mg/ml BSA
Preservative: 0.05% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA1-070 detects munc-18 interacting protein 1 (Mint1) from rat and mouse brain extract as well as extract from HEK293 cells overexpressing the rat gene.

PA1-070 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~130 kDa protein representing Mint1 from rat forebrain postnuclear supernatant.

The PA1-070 immunogen is a synthetic peptide corresponding to residues M(1) N H L E G S A E V E V A D E A P(17) C of rat Mint1. This peptide (Cat. # PEP-157) is available for use in neutralization and control experiments.

General Information
The munc-18 interacting protein (Mint) protein family is a group of evolutionarily conserved adaptor proteins that function in membrane transport and organization. In mammals, there exist three Mint isoforms, Mint1, 2, and 3. Although there is little amino acid sequence conservation in the amino-terminal half, the carboxy-terminal half of these proteins is highly conserved. Within this conserved portion there exists a phosphotyrosine-binding (PTB) and a PSD-95/DLG-A/ZO-1 (PDZ) domain, which function as protein interaction modules. Mint1 and 2 appear to be expressed exclusively in the brain and are found to bind to Munc18, an essential component of the synaptic vesicle fusion machinery. Mint3 is ubiquitously expressed in all tissues and is expressed at the lowest levels in the brain and testis. Studies show that mint3 does not interact with munc-18. Mint3 has been found to interact with the Alzheimer’s Disease-related amyloid precursor protein (APP) and does so through its PTB and PDZ domains. It has been suggested that mint3 links APP to other transport machinery components, thereby regulating it transport, endocytosis, and metabolism. Abnormal APP metabolism has been shown to be the cause of an early-onset type of Alzheimer’s disease.
Product Images
  • Mint1 Antibody (PA1-070) in WB
    PA1-070_1_07232010.jpg

    Western Blot with anti-Mint1 Polyclonal Antibody (PA1-070) (PA1-070_1_07232010.jpg)

    Western Blot with anti-Mint1 Polyclonal Antibody (PA1-070)

    Western blot detection of Mint1 from extract from HEK293 cells overexpressing the rat gene using PA1-070.
Publications:
Immunohistochemistry
  Species / Dilution Summary
  Ms / 1:2,000
PA1-070 was used in immunohistochemistry to investigate the expression and distribution of mint1 and mint2 in adult murine brain

Brain Res Mol Brain Res. 2001 Aug 15;92(1-2):27-42.
"Neuronal expression of mint1 and mint2, novel multimodular proteins, in adult murine brain."
Author(s): Nakajima Y, Okamoto M, Nishimura H, Obata K, Kitano H, Sugita M, Matsuyama T
Number of Citations: 5
(See PubMed article )
  Rt / 1:250
PA1-070 was used in immunohistochemistry to characterize expression of the NR2B-associated trafficking complex in schizophrenia

Schizophr Res. 2010 Jun;119(1-3):198-209.
"Expression of the NR2B-NMDA receptor trafficking complex in prefrontal cortex from a group of elderly patients with schizophrenia."
Author(s): Kristiansen LV, Bakir B, Haroutunian V, Meador-Woodruff JH
Number of Citations: 4
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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