|Host / Isotype:||Rabbit|
|Species Reactivity:||Mouse (Ms) Rat (Rt)|
|Immunogen:||Synthetic peptide corresponding to residues M(1) N H L E G S A E V E V A D E A P(17) C of rat Mint1.|
|Pierce Mint1 Antibody|
|Storage:||-20° C, Avoid Freeze/Thaw Cycles|
|Form:||100 µg of epitope affinity-purified rabbit IgG in PBS containing 1.0 mg/ml BSA and 0.05% sodium azide.|
|Western Blot (WB)||4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
|Product Specific Information|
|PA1-070 detects munc-18 interacting protein 1 (Mint1) from rat and mouse brain extract as well as extract from HEK293 cells overexpressing the rat gene.
PA1-070 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~130 kDa protein representing Mint1 from rat forebrain postnuclear supernatant.
The PA1-070 immunogen is a synthetic peptide corresponding to residues M(1) N H L E G S A E V E V A D E A P(17) C of rat Mint1. This peptide (Cat. # PEP-157) is available for use in neutralization and control experiments.
|The munc-18 interacting protein (Mint) protein family is a group of evolutionarily conserved adaptor proteins that function in membrane transport and organization. In mammals, there exist three Mint isoforms, Mint1, 2, and 3. Although there is little amino acid sequence conservation in the amino-terminal half, the carboxy-terminal half of these proteins is highly conserved. Within this conserved portion there exists a phosphotyrosine-binding (PTB) and a PSD-95/DLG-A/ZO-1 (PDZ) domain, which function as protein interaction modules. Mint1 and 2 appear to be expressed exclusively in the brain and are found to bind to Munc18, an essential component of the synaptic vesicle fusion machinery. Mint3 is ubiquitously expressed in all tissues and is expressed at the lowest levels in the brain and testis. Studies show that mint3 does not interact with munc-18. Mint3 has been found to interact with the Alzheimer’s Disease-related amyloid precursor protein (APP) and does so through its PTB and PDZ domains. It has been suggested that mint3 links APP to other transport machinery components, thereby regulating it transport, endocytosis, and metabolism. Abnormal APP metabolism has been shown to be the cause of an early-onset type of Alzheimer’s disease.|
|Species / Dilution||Summary|
Ms / 1:2,000
PA1-070 was used in immunohistochemistry to investigate the expression and distribution of mint1 and mint2 in adult murine brain
Brain Res Mol Brain Res. 2001 Aug 15;92(1-2):27-42.
"Neuronal expression of mint1 and mint2, novel multimodular proteins, in adult murine brain."
Author(s): Nakajima Y, Okamoto M, Nishimura H, Obata K, Kitano H, Sugita M, Matsuyama T
Number of Citations: 5
(See PubMed article )
Rt / 1:250
PA1-070 was used in immunohistochemistry to characterize expression of the NR2B-associated trafficking complex in schizophrenia
Schizophr Res. 2010 Jun;119(1-3):198-209.
"Expression of the NR2B-NMDA receptor trafficking complex in prefrontal cortex from a group of elderly patients with schizophrenia."
Author(s): Kristiansen LV, Bakir B, Haroutunian V, Meador-Woodruff JH
Number of Citations: 4
(See PubMed article )