Mineralocorticoid Receptor Antibody (H10E4C9F)

Mineralocorticoid Receptor Monoclonal Antibody for Western blot, IF, ICC, IHC, IHC (P), FACS, BLOCK

Synonyms:
Aldosterone Receptor, MR
UniProt ID:
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: H10E4C9F
Tested Species Reactivity: Human (Hu), Rat (Rt), Chicken (Ck), Rabbit (Rb)
Published Species Reactivity: Chicken (Ck), Human (Hu), Mouse (Ms), Ovine (Ov), Rabbit (Rb), Rat (Rt)
Immunogen: Aldosterone-3
Ordering Information
Pierce Mineralocorticoid Receptor Antibody (H10E4C9F)
Product #
MA1-620
Size
100 µl
Price
$360.00
Purchase
Add Mineralocorticoid Receptor Antibody (H10E4C9F) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:1000
Immunofluorescence (IF) 1:100-1:200
Immunocytochemistry (ICC) Assay dependent
Immunohistochemistry (IHC) 1:100
Immunohistochemistry (Paraffin) (IHC (P)) 1/100
Flow Cytometry (FACS) 1/20
Blocking Assay (BLOCK) Assay Dependent
Published Applications Dilution
Western Blot (WB) See publications below
Immunocytochemistry (ICC) See publications below
Immunohistochemistry (IHC) See publications below
Immunoprecipitation (IP) See publications below
ELISA (ELISA) See publications below
Gel Shift (GS) See publications below
Blocking Assay (BLOCK) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1.4mg/ml
Purification: Protein A
Storage Buffer: PBS
Preservative: 0.05% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
MA1-620 detects mineralocorticoid receptor (MR) in human, rat, rabbit and chicken tissues.

MA1-620 has been successfully used in Western blot, immunocytochemistry and immunohistochemistry procedures. By Western blot, this antibody detects a 116 kDa protein representing MR in rat heart homogenate. Immunohistochemical staining of MR in rabbit atrium with MA1-620 results in strong staining of myocytes and endothelial cells. MA1-620 cannot be used for immunoprecipitation.

In immunohistochemical studies, staining with MA1-620 is blocked by pre-incubating the sample with aldosterone. MA1-620 can also be used in formalin-fixed, paraffin-embedded sections. Due to the extremely low levels of MR expressed in native tissues, it is recommended that enhanced detection systems be used for Western blotting such as enhanced chemiluminescence. MA1-620 blocks the binding of aldosterone since it is directed against the steroid binding domain of the mineralocorticoid receptor. The antibody can also react with the steroid binding domain for aldosterone on other receptors.

The MA1-620 immunogen is aldosterone-3. This antibody was produced using the anti-idiotypic method.
General Information
Steroid receptors are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Mineralocorticoids are a family of steroids, secreted by the adrenal cortex, necessary for the regulation of a number of metabolic processes including electrolyte regulation. These compounds exert their effect through their interaction with the mineralocorticoid receptor (MR) and that complex's subsequent association with DNA. Given the function of mineralocorticoids, it is not surprising to find that the kidney is a primary target organ for mineralocorticoids and that this organ has been shown to contain MR.

The corresponding gene for the mineralocorticoid receptor is NR3C2.
Product Images
  • Mineralocorticoid Receptor Antibody (MA1-620) in WB
    NR3C2_Antibody_MA1-620_WesternBlot(WB).jpg

    Western Blot with anti-Mineralocorticoid Receptor Monoclonal Antibody [H10E4C9F] (MA1-620) (NR3C2_Antibody_MA1-620_WesternBlot(WB).jpg)

    Western Blot with anti-Mineralocorticoid Receptor Monoclonal Antibody [H10E4C9F] (MA1-620)

    Western blot of Mineralocorticoid Receptor using MA1-620.
  • Mineralocorticoid Receptor Antibody (MA1-620) in IF
    MA1-620_IF_293.jpg

    Immunofluorescence with anti-Mineralocorticoid Receptor Monoclonal Antibody [H10E4C9F] (MA1-620) (MA1-620_IF_293.jpg)

    Immunofluorescence with anti-Mineralocorticoid Receptor Monoclonal Antibody [H10E4C9F] (MA1-620)

    Immunofluorescent analysis of Mineralocorticoid Receptor using Mineralocorticoid Receptor Monoclonal antibody (H10E4C9F) (Product# MA1-620) shows staining in HEK293 cells. Mineralocorticoid Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Mineralocorticoid Receptor (Product# MA1-620) at a dilution of 1:20-1:200 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
  • Mineralocorticoid Receptor Antibody (MA1-620) in IF
    MA1-620_IF_Hela.jpg

    Immunofluorescence with anti-Mineralocorticoid Receptor Monoclonal Antibody [H10E4C9F] (MA1-620) (MA1-620_IF_Hela.jpg)

    Immunofluorescence with anti-Mineralocorticoid Receptor Monoclonal Antibody [H10E4C9F] (MA1-620)

    Immunofluorescent analysis of Mineralocorticoid Receptor using Mineralocorticoid Receptor Monoclonal antibody (H10E4C9F) (Product# MA1-620) shows staining in HeLa cells. Mineralocorticoid Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Mineralocorticoid Receptor (Product# MA1-620) at a dilution of 1:20-1:200 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
  • Mineralocorticoid Receptor Antibody (MA1-620) in IF
    MA1-620_IF_HepG2.jpg

    Immunofluorescence with anti-Mineralocorticoid Receptor Monoclonal Antibody [H10E4C9F] (MA1-620) (MA1-620_IF_HepG2.jpg)

    Immunofluorescence with anti-Mineralocorticoid Receptor Monoclonal Antibody [H10E4C9F] (MA1-620)

    Immunofluorescent analysis of Mineralocorticoid Receptor using Mineralocorticoid Receptor Monoclonal antibody (H10E4C9F) (Product# MA1-620) shows staining in HepG2 cells. Mineralocorticoid Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Mineralocorticoid Receptor (Product# MA1-620) at a dilution of 1:20-1:200 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
  • Mineralocorticoid Receptor Antibody (MA1-620) in IHC
    MA1-620_Immunohistochemistry_Colon carcinoma.jpg

    Immunohistochemistry with anti-Mineralocorticoid Receptor Monoclonal Antibody [H10E4C9F] (MA1-620) (MA1-620_Immunohistochemistry_Colon carcinoma.jpg)

    Immunohistochemistry with anti-Mineralocorticoid Receptor Monoclonal Antibody [H10E4C9F] (MA1-620)

    Immunohistochemistry was performed on cancer biopsies of deparaffinized Human colon carcinoma tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing Mineralocorticoid Receptor (MA1-620) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  • Mineralocorticoid Receptor Antibody (MA1-620) in IHC
    MA1-620_Immunohistochemistry_Kidney tissue.jpg

    Immunohistochemistry with anti-Mineralocorticoid Receptor Monoclonal Antibody [H10E4C9F] (MA1-620) (MA1-620_Immunohistochemistry_Kidney tissue.jpg)

    Immunohistochemistry with anti-Mineralocorticoid Receptor Monoclonal Antibody [H10E4C9F] (MA1-620)

    Immunohistochemistry was performed on normal deparaffinized Human kidney tissue tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Mineralocorticoid Receptor (MA1-620) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  • Mineralocorticoid Receptor Antibody (MA1-620) in IHC
    MA1-620_Immunohistochemistry_Tonsil tissue.jpg

    Immunohistochemistry with anti-Mineralocorticoid Receptor Monoclonal Antibody [H10E4C9F] (MA1-620) (MA1-620_Immunohistochemistry_Tonsil tissue.jpg)

    Immunohistochemistry with anti-Mineralocorticoid Receptor Monoclonal Antibody [H10E4C9F] (MA1-620)

    Immunohistochemistry was performed on normal deparaffinized Human tonsil tissue tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing Mineralocorticoid Receptor (MA1-620) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Publications:
Western Blot
  Species / Dilution Summary
  Ck / Not Cited
MA1-620 was used in immunocytochemistry and western blot to identify the nuclear receptors associated with somatotroph differentiation and to examine their expression during pituitary development in chicken embryos.

Endocrinology. 2004 Jul;145(7):3523-31.
"Pituitary expression of type I and type II glucocorticoid receptors during chicken embryonic development and their involvement in growth hormone cell differentiation."
Author(s): Bossis I, Nishimura S, Muchow M, Porter TE
Number of Citations: 1
(See PubMed article )
  Hu / 1:400
MA1-620 was used in immunocytochemistry, immunoprecipitation and western blot to investigate the effect of aldosterone and antimineralocorticoids on human mineralocorticosteroid receptor

Biochem J. 1994 Aug 15;302 ( Pt 1)():191-7.
"Differential intracellular localization of human mineralocorticosteroid receptor on binding of agonists and antagonists."
Author(s): Lombès M, Binart N, Delahaye F, Baulieu EE, Rafestin-Oblin ME
Number of Citations: 9
(See PubMed article )
  Hu / Not Cited
MA1-620 was used in immunoprecipitation and western blot to characterize the GR domain (s) involved in the repression of TGF-beta action

J Biol Chem. 2003 Oct 24;278(43):41779-88.
"Identification of glucocorticoid receptor domains involved in transrepression of transforming growth factor-beta action."
Author(s): Li G, Wang S, Gelehrter TD
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
MA1-620 was used in western blot to study the mechanisms of aldosterone-stimulated extracellular matrix deposition in human skin.

J Invest Dermatol. 2010 Oct;130(10):2396-406.
"Aldosterone and mineralocorticoid receptor antagonists modulate elastin and collagen deposition in human skin."
Author(s): Mitts TF, Bunda S, Wang Y, Hinek A
Number of Citations: 2
(See PubMed article )
  Hu / 1:1000
MA1-620 was used in western blot to investigate the molecular mechanisms responsible for the pathogenesis of polycystic ovary syndrome

Exp Clin Endocrinol Diabetes. 2011 Nov;119(10):636-43.
"Hypothalamic-pituitary-adrenocortical axis hypersensitivity and glucocorticoid receptor expression and function in women with polycystic ovary syndrome."
Author(s): Milutinovi? DV, Macut D, Boži? I, Nestorov J, Damjanovi? S, Mati? G
Number of Citations: 1
(See PubMed article )
  Rb / Not Cited
MA1-620 was used in blocking/activating experiment, ELISA and western blot to produce and characterize a monoclonal auto-anti-idiotypic antibody to aldosterone receptors

J Biol Chem. 1989 Feb 15;264(5):2528-36.
"Internal image properties of a monoclonal auto-anti-idiotypic antibody and its binding to aldosterone receptors."
Author(s): Lombes M, Edelman IS, Erlanger BF
Number of Citations: 6
(See PubMed article )
  Rt / 1:1,000
MA1-620 was used in western blot to investigate the effect of cranial irradiation on activation of HPA axis and corticosteroid receptor expression in juvenile rat hippocampus

Gen Physiol Biophys. 2009;28 Spec No():219-27.
"Cranial irradiation modulates hypothalamic-pituitary-adrenal axis activity and corticosteroid receptor expression in the hippocampus of juvenile rat."
Author(s): Velickovic N, Djordjevic A, Drakulic D, Stanojevic I, Secerov B, Horvat A
Number of Citations: 0
(See PubMed article )
  Rt / 1:1,000
MA1-620 was used in immunohistochemistry and western blot to show the presence of mineralocorticoid receptors in the amygdala synapses

PLoS One. 2010 Dec 15;5(12):e14344.
"Localization of mineralocorticoid receptors at mammalian synapses."
Author(s): Prager EM, Brielmaier J, Bergstrom HC, McGuire J, Johnson LR
Number of Citations: 1
(See PubMed article )
Immunocytochemistry
  Species / Dilution Summary
  Ck / 1:250
MA1-620 was used in immunocytochemistry and western blot to identify the nuclear receptors associated with somatotroph differentiation and to examine their expression during pituitary development in chicken embryos.

Endocrinology. 2004 Jul;145(7):3523-31.
"Pituitary expression of type I and type II glucocorticoid receptors during chicken embryonic development and their involvement in growth hormone cell differentiation."
Author(s): Bossis I, Nishimura S, Muchow M, Porter TE
Number of Citations: 1
(See PubMed article )
  Hu / 1:500
MA1-620 was used in immunocytochemistry, immunoprecipitation and western blot to investigate the effect of aldosterone and antimineralocorticoids on human mineralocorticosteroid receptor

Biochem J. 1994 Aug 15;302 ( Pt 1)():191-7.
"Differential intracellular localization of human mineralocorticosteroid receptor on binding of agonists and antagonists."
Author(s): Lombès M, Binart N, Delahaye F, Baulieu EE, Rafestin-Oblin ME
Number of Citations: 9
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  Hu / Not Cited
MA1-620 was used in immunohistochemistry to check MR's expression in human heart at the mRNA and protein level as well as 11-HSD's activity and dependence on its cofactor

Circulation. 1995 Jul 15;92(2):175-82.
"Prerequisite for cardiac aldosterone action. Mineralocorticoid receptor and 11 beta-hydroxysteroid dehydrogenase in the human heart."
Author(s): Lombès M, Alfaidy N, Eugene E, Lessana A, Farman N, Bonvalet JP
Number of Citations: 11
(See PubMed article )
  Hu / 1:300
MA1-620 was used in immunohistochemistry to investigate the expression of mineralocorticoid receptor and 11 beta-hydroxysteroid dehydrogenase in epidermis and appendages

J Clin Endocrinol Metab. 1994 Nov;79(5):1334-41.
"Human skin as target for aldosterone: coexpression of mineralocorticoid receptors and 11 beta-hydroxysteroid dehydrogenase."
Author(s): Kenouch S, Lombes M, Delahaye F, Eugene E, Bonvalet JP, Farman N
Number of Citations: 3
(See PubMed article )
  Hu / 1:25
MA1-620 was used in immunohistochemistry to study the expression of mineralocorticoid receptor in human venous smooth muscle cells

Am J Physiol Heart Circ Physiol. 2011 Jul;301(1):H41-7.
"Mineralocorticoid receptor expression in human venous smooth muscle cells: a potential role for aldosterone signaling in vein graft arterialization."
Author(s): Bafford R, Sui XX, Park M, Miyahara T, Newfell BG, Jaffe IZ, Romero JR, Adler GK, Williams GH, Khalil RA, Conte MS
Number of Citations: 1
(See PubMed article )
  Ms / 1:150
MA1-620 was used in immunohistochemistry to investigate the effect of mineralocorticoid receptor on glucocorticoid receptor at specific stages of mammary gland development.

Mol Endocrinol. 2002 Sep;16(9):2008-18.
"The mineralocorticoid receptor may compensate for the loss of the glucocorticoid receptor at specific stages of mammary gland development."
Author(s): Kingsley-Kallesen M, Mukhopadhyay SS, Wyszomierski SL, Schanler S, Schütz G, Rosen JM
Number of Citations: 5
(See PubMed article )
  Ov / 1:200
MA1-620 was used in immunohistochemistry to investigate the distribution of mineralocorticoid receptor in the lung of late-gestation sheep fetus

J Soc Gynecol Investig. 2005 Feb;12(2):84-91.
"Mineralocorticoid receptor expression in late-gestation ovine fetal lung."
Author(s): Keller-Wood M, Wood CE, Hua Y, Zhang D
Number of Citations: 1
(See PubMed article )
  Rb / 1-5 ug/ml
MA1-620 was used in EMSA assay and immunohistochemistry to investigate the expression and distribution of mineralocorticoid receptor in the rabbit heart and blood vessels

Circ Res. 1992 Sep;71(3):503-10.
"Immunohistochemical and biochemical evidence for a cardiovascular mineralocorticoid receptor."
Author(s): Lombès M, Oblin ME, Gasc JM, Baulieu EE, Farman N, Bonvalet JP
Number of Citations: 11
(See PubMed article )
  Rb / 1-5 ug/ml
MA1-620 was used in immunohistochemistry to investigate the distribution of mineralocorticoid receptor in rabbit kidney

Proc Natl Acad Sci U S A. 1990 Feb;87(3):1086-8.
"Immunohistochemical localization of renal mineralocorticoid receptor by using an anti-idiotypic antibody that is an internal image of aldosterone."
Author(s): Lombès M, Farman N, Oblin ME, Baulieu EE, Bonvalet JP, Erlanger BF, Gasc JM
Number of Citations: 10
(See PubMed article )
  Rt / 1:100
MA1-620 was used in immunohistochemistry to investigate the role of mineralocorticoid receptor on testosterone production in rat Leydig cells

Mol Cell Endocrinol. 2005 Nov 24;243(1-2):35-42.
"Stimulation of testosterone production in rat Leydig cells by aldosterone is mineralocorticoid receptor mediated."
Author(s): Ge RS, Dong Q, Sottas CM, Latif SA, Morris DJ, Hardy MP
Number of Citations: 1
(See PubMed article )
  Rt / 1:100
MA1-620 was used in immunohistochemistry and western blot to show the presence of mineralocorticoid receptors in the amygdala synapses

PLoS One. 2010 Dec 15;5(12):e14344.
"Localization of mineralocorticoid receptors at mammalian synapses."
Author(s): Prager EM, Brielmaier J, Bergstrom HC, McGuire J, Johnson LR
Number of Citations: 1
(See PubMed article )
Immunoprecipitation
  Species / Dilution Summary
  Hu / Not Cited
MA1-620 was used in immunocytochemistry, immunoprecipitation and western blot to investigate the effect of aldosterone and antimineralocorticoids on human mineralocorticosteroid receptor

Biochem J. 1994 Aug 15;302 ( Pt 1)():191-7.
"Differential intracellular localization of human mineralocorticosteroid receptor on binding of agonists and antagonists."
Author(s): Lombès M, Binart N, Delahaye F, Baulieu EE, Rafestin-Oblin ME
Number of Citations: 9
(See PubMed article )
  Hu / Not Cited
MA1-620 was used in immunoprecipitation and western blot to characterize the GR domain (s) involved in the repression of TGF-beta action

J Biol Chem. 2003 Oct 24;278(43):41779-88.
"Identification of glucocorticoid receptor domains involved in transrepression of transforming growth factor-beta action."
Author(s): Li G, Wang S, Gelehrter TD
Number of Citations: 1
(See PubMed article )
  Rt / Not Cited
MA1-620 was used in immunoprecipitation to investigate the role of p23 molecular chaperones in intracellular receptor function in animal and yeast cells.

Genes Dev. 2000 Feb 15;14(4):422-34.
"The p23 molecular chaperones act at a late step in intracellular receptor action to differentially affect ligand efficacies."
Author(s): Freeman BC, Felts SJ, Toft DO, Yamamoto KR
Number of Citations: 21
(See PubMed article )
ELISA
  Species / Dilution Summary
  Rb / Not Cited
MA1-620 was used in blocking/activating experiment, ELISA and western blot to produce and characterize a monoclonal auto-anti-idiotypic antibody to aldosterone receptors

J Biol Chem. 1989 Feb 15;264(5):2528-36.
"Internal image properties of a monoclonal auto-anti-idiotypic antibody and its binding to aldosterone receptors."
Author(s): Lombes M, Edelman IS, Erlanger BF
Number of Citations: 6
(See PubMed article )
Gel Shift
  Species / Dilution Summary
  Rb / 1:100
MA1-620 was used in EMSA assay and immunohistochemistry to investigate the expression and distribution of mineralocorticoid receptor in the rabbit heart and blood vessels

Circ Res. 1992 Sep;71(3):503-10.
"Immunohistochemical and biochemical evidence for a cardiovascular mineralocorticoid receptor."
Author(s): Lombès M, Oblin ME, Gasc JM, Baulieu EE, Farman N, Bonvalet JP
Number of Citations: 11
(See PubMed article )
Blocking Assay
  Species / Dilution Summary
  Ck / 1:100
MA1-620 was used in blocking/activating experiment to investigate the changes of the mineralocorticosteroid receptor stability with aldosterone stimulation

Biochem J. 1992 Mar 15;282 ( Pt 3)():697-702.
"Aldosterone antagonists destabilize the mineralocorticosteroid receptor."
Author(s): Couette B, Lombes M, Baulieu EE, Rafestin-Oblin ME
Number of Citations: 5
(See PubMed article )
  Rb / Not Cited
MA1-620 was used in blocking/activating experiment, ELISA and western blot to produce and characterize a monoclonal auto-anti-idiotypic antibody to aldosterone receptors

J Biol Chem. 1989 Feb 15;264(5):2528-36.
"Internal image properties of a monoclonal auto-anti-idiotypic antibody and its binding to aldosterone receptors."
Author(s): Lombes M, Edelman IS, Erlanger BF
Number of Citations: 6
(See PubMed article )
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