2 mg of purified IgY (1 mg/ml) (deltaFc) in 20mM MES (p.H. 5.8) with 0.1% sodium azide.
Applications
Dilution *
Immunodiffusion (IDF)
Assay Dependent
ELISA (ELISA)
1:5,000
Western Blot (WB)
1:1,000
Immunoelectrophoresis (IE)
Assay Dependent
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
D0206-2B detects Midkine from human samples.
D0206-2B has been successfully used in ELISA and WB applications.
The D0206-2B immunogen is recombinant Midkine.
Figure 1 illustrates Western blot detection of both reduced and non-reduced Midkine using D0206-2B
General Information
Product Images
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WB using D0206-2B
Western Blot with anti-Midkine Polyclonal Antibody (D0206-2B)