Methyl CpG Binding Protein 2 Antibody

Methyl CpG Binding Protein 2 Polyclonal Antibody for Western blot, IF, ICC, IHC (P), IP

>> See 7 other antibodies for MECP2 (MeCP2)
Synonyms:
MeCP2
Entrez Gene ID:
Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt)
Published Species Reactivity: Amphibian (Am), Human (Hu), Non-human primate (Nhp), Rat (Rt)
Immunogen: Synthetic Peptide: M(1) V A G M L G L R E E K S E D(15) C
Ordering Information
Pierce Methyl CpG Binding Protein 2 Antibody
Product #
PA1-887
Size
50 µg
Price
$360.00
Purchase
Add Methyl CpG Binding Protein 2 Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 0.5 µg/ml
Immunofluorescence (IF) 1:100-1:200
Immunocytochemistry (ICC) 1:100-1:200
Immunohistochemistry (Paraffin) (IHC (P)) 1:200-1:2000
Immunoprecipitation (IP) Assay dependent
Published Applications Dilution
Western Blot (WB) See publications below
Immunocytochemistry (ICC) See publications below
Immunohistochemistry (IHC) See publications below
Immunoprecipitation (IP) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: Antigen affinity chromatography
Storage Buffer: PBS with 1mg/ml BSA
Preservative: 0.05% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA1-887 detects methyl CpG binding protein 2 (MeCP2) from human, mouse and rat tissues and cells.

PA1-887 has been successfully used in Western blot, IHC-P, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects an ~56 kDa protein representing MeCP2 from AtT20 cell extract.

PA1-887 immunizing peptide corresponds to amino acid residues 1-15 from mouse MeCP2. This sequence is completely conserved in human MeCP2. PA1-887 immunizing peptide (Cat. # PEP-120) is available for use in neutralization and control experiments.
General Information
DNA methyltransferases methylate the 5-position of cytosine in the context of CpG dinucleotides. DNA methylation is crucial for normal embryonic development, imprinting, and X-chromosome inactivation. Methyl CpG binding proteins (MeCPs) specifically recognize methylated regions of DNA and repress transcription both directly and by association with known corepressor proteins which include members of the Sin3 and histone deactylase protein families.
Product Images
  • Methyl CpG Binding Protein 2 Antibody (PA1-887) in WB
    PA1-887_1_07232010.jpg

    Western Blot with anti-Methyl CpG Binding Protein 2 Polyclonal Antibody (PA1-887) (PA1-887_1_07232010.jpg)

    Western Blot with anti-Methyl CpG Binding Protein 2 Polyclonal Antibody (PA1-887)

    Western blot of MeCP2 from HeLa cell extract using PA1-887.
  • Methyl CpG Binding Protein 2 Antibody (PA1-887) in IF
    PA1-887_IF_C6.jpg

    Immunofluorescence with anti-Methyl CpG Binding Protein 2 Polyclonal Antibody (PA1-887) (PA1-887_IF_C6.jpg)

    Immunofluorescence with anti-Methyl CpG Binding Protein 2 Polyclonal Antibody (PA1-887)

    Immunofluorescent analysis of Methyl CpG Binding Protein 2 (green) showing staining in the nucleus of C6 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Methyl CpG Binding Protein 2 polyclonal antibody (Product # PA1-887) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.
  • Methyl CpG Binding Protein 2 Antibody (PA1-887) in IF
    PA1-887_IF_C2C12.jpg

    Immunofluorescence with anti-Methyl CpG Binding Protein 2 Polyclonal Antibody (PA1-887) (PA1-887_IF_C2C12.jpg)

    Immunofluorescence with anti-Methyl CpG Binding Protein 2 Polyclonal Antibody (PA1-887)

    Immunofluorescent analysis of Methyl CpG Binding Protein 2 (green) showing staining in the nucleus of C2C12 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Methyl CpG Binding Protein 2 polyclonal antibody (Product # PA1-887) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Actin was stained using Alexa Fluor 554 (red) and nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.
  • Methyl CpG Binding Protein 2 Antibody (PA1-887) in IHC (P)
    PA1-887_IHC_mouse_brain_tissue.jpg

    Immunohistochemistry (Paraffin) with anti-Methyl CpG Binding Protein 2 Polyclonal Antibody (PA1-887) (PA1-887_IHC_mouse_brain_tissue.jpg)

    Immunohistochemistry (Paraffin) with anti-Methyl CpG Binding Protein 2 Polyclonal Antibody (PA1-887)

    Immunohistochemistry analysis of Methyl CpG Binding Protein 2 showing staining in the nucleus of paraffin-embedded mouse brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Methyl CpG Binding Protein 2 polyclonal antibody (Product # PA1-887) diluted in 3% BSA-PBS at a dilution of 1:500 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
  • Methyl CpG Binding Protein 2 Antibody (PA1-887) in IHC (P)
    PA1-887_IHC_rat_brain_tissue.jpg

    Immunohistochemistry (Paraffin) with anti-Methyl CpG Binding Protein 2 Polyclonal Antibody (PA1-887) (PA1-887_IHC_rat_brain_tissue.jpg)

    Immunohistochemistry (Paraffin) with anti-Methyl CpG Binding Protein 2 Polyclonal Antibody (PA1-887)

    Immunohistochemistry analysis of Methyl CpG Binding Protein 2 showing staining in the nucleus of paraffin-embedded rat brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Methyl CpG Binding Protein 2 polyclonal antibody (Product # PA1-887) diluted in 3% BSA-PBS at a dilution of 1:1000 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Publications:
Western Blot
  Species / Dilution Summary
  Am / Not Cited
PA1-887 was used in immunoprecipitation and western blot to investigate the influence of MeCP2 on transcriptional silencing of methylated DNA

Nat Genet. 1998 Jun;19(2):187-91.
"Methylated DNA and MeCP2 recruit histone deacetylase to repress transcription."
Author(s): Jones PL, Veenstra GJ, Wade PA, Vermaak D, Kass SU, Landsberger N, Strouboulis J, Wolffe AP
Number of Citations: 1
(See PubMed article )
  Hu / 1:5000
PA1-887 was used in western blot to study the domain structure of methyl-CpG-binding protein MeCP2 and its involvement in Rett syndrome

J Mol Med (Berl). 2004 Feb;82(2):135-43.
"A WW domain binding region in methyl-CpG-binding protein MeCP2: impact on Rett syndrome."
Author(s): Buschdorf JP, Strätling WH
Number of Citations: 1
(See PubMed article )
  Hu / 1:1,000
PA1-887 was used in immunohistochemistry and western blot to identify those genes able to compensate for changes in MeCP2's expression level

PLoS Genet. 2008 Sep 5;4(9):e1000179.
"Genetic modifiers of MeCP2 function in Drosophila."
Author(s): Cukier HN, Perez AM, Collins AL, Zhou Z, Zoghbi HY, Botas J
Number of Citations: 1
(See PubMed article )
  Hu / 1:2000
PA1-887 was used in immunocytochemistry and western blot to investigate the effect of methyl-CpG-binding protein-2 on intestinal stem cell proliferation in Drosophila

Cell Struct Funct. 2011;36(2):197-208.
"Regulation of intestinal stem cell proliferation by human methyl-CpG-binding protein-2 in Drosophila."
Author(s): Lee SH, Kim IJ, Kim JG, Park JS, Kim YS, Yamaguchi M, Kim CM, Yoo MA
Number of Citations: 0
(See PubMed article )
  Rt / 0
PA1-887 was used in western blot to develop a rat model with lowered Mecp2 expression in the brain

Int J Dev Neurosci. 2008 Aug;26(5):457-65.
"RNAi-induced down-regulation of Mecp2 expression in the rat brain."
Author(s): Jin J, Bao X, Wang H, Pan H, Zhang Y, Wu X
Number of Citations: 1
(See PubMed article )
Immunocytochemistry
  Species / Dilution Summary
  Hu / 1:300
PA1-887 was used in immunocytochemistry and western blot to investigate the effect of methyl-CpG-binding protein-2 on intestinal stem cell proliferation in Drosophila

Cell Struct Funct. 2011;36(2):197-208.
"Regulation of intestinal stem cell proliferation by human methyl-CpG-binding protein-2 in Drosophila."
Author(s): Lee SH, Kim IJ, Kim JG, Park JS, Kim YS, Yamaguchi M, Kim CM, Yoo MA
Number of Citations: 0
(See PubMed article )
  Nhp / 0
PA1-887 was used in immunocytochemistry to investigate a missense mutation in MeCP2_e1 isoform in Rett syndrome

Neurogenetics. 2009 Apr;10(2):127-33.
"The first missense mutation causing Rett syndrome specifically affecting the MeCP2_e1 isoform."
Author(s): Fichou Y, Nectoux J, Bahi-Buisson N, Rosas-Vargas H, Girard B, Chelly J, Bienvenu T
Number of Citations: 1
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  Hu / 1:100
PA1-887 was used in immunohistochemistry to examine the expression profile of methyl-CpG-binding protein 2 in normal and Rett syndrome brains.

Hum Mol Genet. 2001 Aug 15;10(17):1729-40.
"Quantitative localization of heterogeneous methyl-CpG-binding protein 2 (MeCP2) expression phenotypes in normal and Rett syndrome brain by laser scanning cytometry."
Author(s): LaSalle JM, Goldstine J, Balmer D, Greco CM
Number of Citations: 13
(See PubMed article )
  Hu / Not Cited
PA1-887 was used in immunohistochemistry to study the regulatory mechanisms of the MeCP2 expression during brain development

Hum Mol Genet. 2004 Mar 15;13(6):629-39.
"Multiple pathways regulate MeCP2 expression in normal brain development and exhibit defects in autism-spectrum disorders."
Author(s): Samaco RC, Nagarajan RP, Braunschweig D, LaSalle JM
Number of Citations: 1
(See PubMed article )
  Hu / 1:100
PA1-887 was used in immunohistochemistry and western blot to identify those genes able to compensate for changes in MeCP2's expression level

PLoS Genet. 2008 Sep 5;4(9):e1000179.
"Genetic modifiers of MeCP2 function in Drosophila."
Author(s): Cukier HN, Perez AM, Collins AL, Zhou Z, Zoghbi HY, Botas J
Number of Citations: 1
(See PubMed article )
Immunoprecipitation
  Species / Dilution Summary
  Am / Not Cited
PA1-887 was used in immunoprecipitation and western blot to investigate the influence of MeCP2 on transcriptional silencing of methylated DNA

Nat Genet. 1998 Jun;19(2):187-91.
"Methylated DNA and MeCP2 recruit histone deacetylase to repress transcription."
Author(s): Jones PL, Veenstra GJ, Wade PA, Vermaak D, Kass SU, Landsberger N, Strouboulis J, Wolffe AP
Number of Citations: 1
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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