MSH6 Antibody (3E1)

MSH6 Monoclonal Antibody for Western blot, IF, ICC, IHC, FACS

>> See 8 other antibodies for MSH6
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgG2a
Class: Monoclonal
Type: Antibody
Clone: 3E1
Tested Species Reactivity: Human (Hu)
Immunogen: Purified recombinant fragment of human MSH6 expressed in E. Coli.
Ordering Information
Pierce MSH6 Antibody (3E1)
Product #
MA5-17129
Size
100 ul
Price
$310.00
Purchase
Add MSH6 Antibody (3E1) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:500 - 1:2000
Immunofluorescence (IF) 1:200 - 1:1000
Immunocytochemistry (ICC) 1:200 - 1:1000
Immunohistochemistry (IHC) 1:200 - 1:1000
Flow Cytometry (FACS) 1:200 - 1:400
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Storage Buffer: ascites
Preservative: 0.03% sodium azide
Storage Conditions: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Specific Information
MA5-17129 targets MSH6 in FACS, ICC, IHC, pep-ELISA, and WB applications and shows reactivity with Human samples.

The MA5-17129 immunogen is purified recombinant fragment of human MSH6 expressed in E. Coli.

MA5-17129 detects MSH6 which has a predicted molecular weight of approximately 160kDa.
General Information
This gene encodes a protein similar to the MutS protein. In E. coli, the MutS protein helps in the recognition of mismatched nucleotides, prior to their repair. A highly conserved region of approximately 150 aa, called the Walker-A adenine nucleotide binding motif, exists in MutS homologs. The encoded protein of this gene combines with MSH2 to form a mismatch recognition complex that functions as a bidirectional molecular switch that exchanges ADP and ATP as DNA mismatches are bound and dissociated. Mutations in this gene have been identified in individuals with hereditary nonpolyposis colon cancer (HNPCC) and endometrial cancer.
Product Images
  • MSH6 Antibody (MA5-17129) in WB
    MSH6_Antibody_MA5-17129_Westernblot1-1252013.jpg

    Western Blot with anti-MSH6 Monoclonal Antibody [3E1] (MA5-17129) (MSH6_Antibody_MA5-17129_Westernblot1-1252013.jpg)

    Western Blot with anti-MSH6 Monoclonal Antibody [3E1] (MA5-17129)

    Western blot analysis of MSH6 using a MSH6 monoclonal antibody (Product # MA5-17129) against a human MSH6 (AA: 217-395) recombinant protein.
  • MSH6 Antibody (MA5-17129) in WB
    MSH6_Antibody_MA5-17129_Westernblot2-1252013.jpg

    Western Blot with anti-MSH6 Monoclonal Antibody [3E1] (MA5-17129) (MSH6_Antibody_MA5-17129_Westernblot2-1252013.jpg)

    Western Blot with anti-MSH6 Monoclonal Antibody [3E1] (MA5-17129)

    Western blot analysis of MSH6 using MSH6 monoclonal antibody (Product # MA5-17129) in HEK293 (1), HCT116 (2), A549 (3), A431 (4), MCF-7 (5) and HepG2 (6) cell lysate.
  • MSH6 Antibody (MA5-17129) in IF
    MSH6_Antibody_MA5-17129_Immunofluorescence1-1252013.jpg

    Immunofluorescence with anti-MSH6 Monoclonal Antibody [3E1] (MA5-17129) (MSH6_Antibody_MA5-17129_Immunofluorescence1-1252013.jpg)

    Immunofluorescence with anti-MSH6 Monoclonal Antibody [3E1] (MA5-17129)

    Immunofluorescence analysis of HeLa cells using MSH6 monoclonal antibody (Product # MA5-17129) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.
  • MSH6 Antibody (MA5-17129) in IHC
    MSH6_Antibody_MA5-17129_Immunohistochemical1-1252013.jpg

    Immunohistochemistry with anti-MSH6 Monoclonal Antibody [3E1] (MA5-17129) (MSH6_Antibody_MA5-17129_Immunohistochemical1-1252013.jpg)

    Immunohistochemistry with anti-MSH6 Monoclonal Antibody [3E1] (MA5-17129)

    Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using MSH6 monoclonal antibody (Product # MA5-17129) followed with DAB staining.
  • MSH6 Antibody (MA5-17129) in IHC
    MSH6_Antibody_MA5-17129_Immunohistochemical2-1252013.jpg

    Immunohistochemistry with anti-MSH6 Monoclonal Antibody [3E1] (MA5-17129) (MSH6_Antibody_MA5-17129_Immunohistochemical2-1252013.jpg)

    Immunohistochemistry with anti-MSH6 Monoclonal Antibody [3E1] (MA5-17129)

    Immunohistochemical analysis of paraffin-embedded colon cancer tissues using MSH6 monoclonal antibody (Product # MA5-17129) followed with DAB staining.
  • MSH6 Antibody (MA5-17129) in FACS
    MSH6_Antibody_MA5-17129_FlowCytometry-1252013.jpg

    Flow Cytometry with anti-MSH6 Monoclonal Antibody [3E1] (MA5-17129) (MSH6_Antibody_MA5-17129_FlowCytometry-1252013.jpg)

    Flow Cytometry with anti-MSH6 Monoclonal Antibody [3E1] (MA5-17129)

    Flow cytometric analysis of MCF-7 cells using MSH6 monoclonal antibody (Product # MA5-17129) (green) and negative control (red).
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific