MMP-10 (Stromelysin-2) Antibody (MA5-14233) in IP
MA5-14233_Immunoprecipitation.jpgImmunoprecipitation of MMP-10 (Stromelysin-2) using MMP-10 (Stromelysin-2) Monoclonal Antibody (MA5-14233) on Native Human U937 Cells.
|Host / Isotype:||Mouse / IgG2b|
|Tested Species Reactivity:||Human (Hu)|
|Published Species Reactivity:||Human (Hu)|
|Immunogen:||Human recombinant MMP-10 (stromelysin-2 protein)|
|Pierce MMP-10 (Stromelysin-2) Antibody (IVC5)|
|Tested Applications||Dilution *|
|Western Blot (WB)||1-2 µg/ml|
|Immunoprecipitation (IP)||2 µg/ml|
|Immunohistochemistry (IHC)||See publications below|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
|Storage Buffer:||PBS, pH 7.4, with 0.2% BSA|
|Preservative:||0.09% sodium azide|
|Storage Conditions:||4° C, do not freeze|
|Product Specific Information|
|MA5-14233 targets MMP-10 (Stromelysin-2) in IP and WB applications and shows reactivity with Human samples.
The MA5-14233 immunogen is human recombinant MMP-10 (stromelysin-2 protein).
|Matrix metalloproteinases (MMP) are proteolytic enzymes capable of degrading extracellular matrix. Stromelysin, a member of the matrix metalloproteinase family, demonstrates wide substrate specificity with the ability to degrade proteoglycan, fibronectin, laminin, casein, and the nonhelical region of collagen. The two forms of stromelysin (SL), types 1 (MMP-3) and 2 (MMP-10), share 82% sequence homology, but exhibit differences in cellular synthesis and inducibility by cytokines and growth factors in vitro.|
|Species / Dilution||Summary|
Hu / 0
MA5-14233 was used in immunohistochemistry to study changes in corneal extracellular matrix and MMPs following complicated laser-assisted in situ keratomileusis
Cornea. 2002 Jan;21(1):95-100.
"Extracellular matrix and matrix metalloproteinase changes in human corneas after complicated laser-assisted in situ keratomileusis (LASIK)."
Author(s): Maguen E, Zorapapel NC, Zieske JD, Ninomiya Y, Sado Y, Kenney MC, Ljubimov AV
Number of Citations: 4
(See PubMed article )
Hu / 2 ug/ml
MA5-14233 was used in immunohistochemistry to investigate the expression and the role of matrix metalloproteinases in intestinal fistulae
Gut. 2004 May;53(5):701-9.
"Expression and localisation of matrix metalloproteinases and their natural inhibitors in fistulae of patients with Crohn's disease."
Author(s): Kirkegaard T, Hansen A, Bruun E, Brynskov J
Number of Citations: 8
(See PubMed article )