MIF Antibody

(This product has been discontinued.)
Please click the link below to find suitable replacements for this discontinued product.

>> See 5 other antibodies for MIF

MIF Polyclonal Antibody for Western blot, IF, FACS

>> See 5 other antibodies for MIF (GIF, GLIF, MMIF, L-dopachrome isomerase, L-dopachrome tautomerase, macrophage migration inhibitory factor, phenylpyruvate tautomerase)
Synonyms:
GIF, GLIF, MMIF, L-dopachrome isomerase, L-dopachrome tautomerase, macrophage migration inhibitory factor, phenylpyruvate tautomerase
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit / IgG
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu)
Immunogen: Synthetic peptide from the C-terminal region of human MIF conjugated to KLH.
Ordering Information
Pierce MIF Antibody
Product #
PA5-25026
Size
400 ul
Price
$316.00
Purchase
(DISCONTINUED)
Tested Applications Dilution *
Western Blot (WB) 1:100-500
Immunofluorescence (IF) 1:10-50
Flow Cytometry (FACS) 1:10-50
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: Lot-specific
Purification: Antigen affinity chromatography
Storage Buffer: PBS
Preservative: 0.09% sodium azide
Storage Conditions: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Product Specific Information
PA5-25026 targets MIF in FACS, IF, and WB applications and shows reactivity with Human samples.

The PA5-25026 immunogen is synthetic peptide from the C-terminal region of human MIF conjugated to KLH.
General Information
MIF (macrophage migration inhibitory factor) was one of the first cytokine activities to be discovered and was initially described as a T cell-derived factor that inhibits the random migration of macrophages (Weiser 1989). Recently, MIF was rediscovered as a pituitary hormone that act as the counterregulatory hormone for glucocorticoid action within the immune system (Bernhagen 1993, Mitchell 1995). MIF was released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production (Bucala 1998). MIF plays a critical role in the host control of inflammation and immunity.
Product Images
  • MIF Antibody (PA5-25026) in WB
    MIF-_Antibody_PA5-25026_WB_1_112013.jpg

    Western Blot with anti-MIF  Polyclonal Antibody (PA5-25026) (MIF-_Antibody_PA5-25026_WB_1_112013.jpg)

    Western Blot with anti-MIF Polyclonal Antibody (PA5-25026)

    Western blot analysis of anti-MIF Polyclonal Antibody (PA5-25026) in MCF-7 cell line lysates (35 ug per lane).
  • MIF Antibody (PA5-25026) in IF
    MIF-_Antibody_PA5-25026_IF_1_112013.jpg

    Immunofluorescence with anti-MIF  Polyclonal Antibody (PA5-25026) (MIF-_Antibody_PA5-25026_IF_1_112013.jpg)

    Immunofluorescence with anti-MIF Polyclonal Antibody (PA5-25026)

    Immunofluorescent analysis of MCF-7 cells using a MIF polyclonal antibody (PA5-25026) at a dilution of 1:10-50. Primary antibody was detected with goat anti-rabbit lgG, fluor-conjugated secondary antibody (green). DAPI was used to stain the cells nuclear (blue).
  • MIF Antibody (PA5-25026) in FACS
    MIF-_Antibody_PA5-25026_FC_1_112013.jpg

    Flow Cytometry with anti-MIF  Polyclonal Antibody (PA5-25026) (MIF-_Antibody_PA5-25026_FC_1_112013.jpg)

    Flow Cytometry with anti-MIF Polyclonal Antibody (PA5-25026)

    Flow cytometric analysis of MCF-7 cells using anti-MIF Polyclonal Antibody (PA5-25026) (right) compared to a negative control cell (left) at a dilution of 1:10-50. Secondary antibody used to visualize was a Goat Anti-Rabbit Secondary Antibody Conjugated to FITC.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific