Synthetic peptide from the C-terminal region of human MIF conjugated to KLH.
Pierce MIF Antibody
Western Blot (WB)
Flow Cytometry (FACS)
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Antigen affinity chromatography
0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Product Specific Information
PA5-25026 targets MIF in FACS, IF, and WB applications and shows reactivity with Human samples.
The PA5-25026 immunogen is synthetic peptide from the C-terminal region of human MIF conjugated to KLH.
MIF (macrophage migration inhibitory factor) was one of the first cytokine activities to be discovered and was initially described as a T cell-derived factor that inhibits the random migration of macrophages (Weiser 1989). Recently, MIF was rediscovered as a pituitary hormone that act as the counterregulatory hormone for glucocorticoid action within the immune system (Bernhagen 1993, Mitchell 1995). MIF was released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production (Bucala 1998). MIF plays a critical role in the host control of inflammation and immunity.
MIF Antibody (PA5-25026) in WB MIF-_Antibody_PA5-25026_WB_1_112013.jpg
Western Blot with anti-MIF Polyclonal Antibody (PA5-25026)
Immunofluorescent analysis of MCF-7 cells using anti-MIF Polyclonal Antibody (PA5-25026) at a dilution of 1:10-50. Primary antibody was detected with goat anti-rabbit lgG, Alexa Fluor 488 conjugated secondary antibody (green). DAPI was used to stain the cells nuclear (blue).
MIF Antibody (PA5-25026) in FACS MIF-_Antibody_PA5-25026_FC_1_112013.jpg
Flow Cytometry with anti-MIF Polyclonal Antibody (PA5-25026)
Flow cytometric analysis of MCF-7 cells using anti-MIF Polyclonal Antibody (PA5-25026) (right) compared to a negative control cell (left) at a dilution of 1:10-50. Secondary antibody used to visualize was a Goat Anti-Rabbit Secondary Antibody Conjugated to FITC.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)