MHC II (HLA-DQ) Antibody (SPV-L3)

MHC II (HLA-DQ) Monoclonal Antibody for Western blot, IF, ICC, IHC (F), FACS

Synonyms:
DC-1 alpha chain, DC-alpha, HLA class II histocompatibility antigen, DQ alpha 1 chain, HLA class II histocompatibility antigen, DQ(W3) alpha chain, HLA-DCA, MHC HLA-DQ alpha, MHC class II HLA-D alpha glycoprotein, MHC class II HLA-DQ-alpha-1, MHC class II antigen, MHC class II surface glycoprotein,leucocyte antigen DQA1, leukocyte antigen alpha chain
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgG2a
Class: Monoclonal
Type: Antibody
Clone: SPV-L3
Tested Species Reactivity: Human (Hu), Mouse (Ms), Porcine (Po)
Published Species Reactivity: Human (Hu), Mouse (Ms)
Immunogen: T4-positive CTL clone HG-38
Ordering Information
Pierce MHC II (HLA-DQ) Antibody (SPV-L3)
Product #
MA5-12298
Size
500 ul
Price
$255.00
Purchase
Add MHC II (HLA-DQ) Antibody (SPV-L3) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:10-1:200
Immunofluorescence (IF) 1:10-1:100
Immunocytochemistry (ICC) 1:10-1:100
Immunohistochemistry (Frozen) (IHC (F)) Assay Dependent
Flow Cytometry (FACS) 2 ug/test
Published Applications Dilution
Immunohistochemistry (IHC) See publications below
Blocking Assay (BLOCK) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 0.2mg/ml
Purification: Protein A
Storage Buffer: PBS, pH 7.4, with 0.2% BSA
Preservative: 0.09% sodium azide
Storage Conditions: 4° C, do not freeze
Product Specific Information
MA5-12298 targets MHC II (HLA-DQ) in FACS, IF, WB and IHC (F) applications and shows reactivity with Human, Mouse and Porcine samples.

The MA5-12298 immunogen is t4-positive CTL clone HG-38.
General Information
MHC class II antigens are transmembrane glycoproteins of non-covalently linked alpha (33-35kDa) and beta (27-30kDa) chains. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They therefore have a critical role in the initiation of the immune response. Differential expression of MHC class II antigens on fetal and adult lymphocytes, malignant B cells appears to reflect the stage of cell differentiation which may be useful in the study of lymphoproliferative disorders.
Product Images
  • MHC II (HLA-DQ) Antibody (MA5-12298) in WB
    MA5-12298_WB.jpg

    Western Blot with anti-MHC II (HLA-DQ) Monoclonal Antibody [SPV-L3] (MA5-12298) (MA5-12298_WB.jpg)

    Western Blot with anti-MHC II (HLA-DQ) Monoclonal Antibody [SPV-L3] (MA5-12298)

    Western blot analysis of MHC II (HLA-DQ) was performed by loading 25 ug of Daudi (lane 1) and Raji (lane 2) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a MHC II (HLA-DQ) monoclonal antibody (Product # MA5-12298) at a dilution of 1:100 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~27-30 kDa.
  • MHC II (HLA-DQ) Antibody (MA5-12298) in IF
    MA5-12298_IF_NIH-3T3_20131018121442.jpg

    Immunofluorescence with anti-MHC II (HLA-DQ) Monoclonal Antibody [SPV-L3] (MA5-12298) (MA5-12298_IF_NIH-3T3_20131018121442.jpg)

    Immunofluorescence with anti-MHC II (HLA-DQ) Monoclonal Antibody [SPV-L3] (MA5-12298)

    Immunofluorescent analysis of MHC II (HLA-DQ) (green) showing staining in the cytoplasm and membrane of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a MHC II (HLA-DQ) monoclonal antibody (Product # MA5-12298) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
  • MHC II (HLA-DQ) Antibody (MA5-12298) in IF
    MA5-12298_IF_BAF-3_20131018121459.jpg

    Immunofluorescence with anti-MHC II (HLA-DQ) Monoclonal Antibody [SPV-L3] (MA5-12298) (MA5-12298_IF_BAF-3_20131018121459.jpg)

    Immunofluorescence with anti-MHC II (HLA-DQ) Monoclonal Antibody [SPV-L3] (MA5-12298)

    Immunofluorescent analysis of MHC II (HLA-DQ) (green) showing staining in the cytoplasm of BAF-3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a MHC II (HLA-DQ) monoclonal antibody (Product # MA5-12298) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
  • MHC II (HLA-DQ) Antibody (MA5-12298) in IF
    MA5-12298_IF_Raji_20131018121516.jpg

    Immunofluorescence with anti-MHC II (HLA-DQ) Monoclonal Antibody [SPV-L3] (MA5-12298) (MA5-12298_IF_Raji_20131018121516.jpg)

    Immunofluorescence with anti-MHC II (HLA-DQ) Monoclonal Antibody [SPV-L3] (MA5-12298)

    Immunofluorescent analysis of MHC II (HLA-DQ) (green) showing staining in the golgi of Raji cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a MHC II (HLA-DQ) monoclonal antibody (Product # MA5-12298) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
  • MHC II (HLA-DQ) Antibody (MA5-12298) in IHC
    MA5-12298_Immunohistochemistry.jpg

    Immunohistochemistry with anti-MHC II (HLA-DQ) Monoclonal Antibody [SPV-L3] (MA5-12298) (MA5-12298_Immunohistochemistry.jpg)

    Immunohistochemistry with anti-MHC II (HLA-DQ) Monoclonal Antibody [SPV-L3] (MA5-12298)

    Frozen human tonsil section stained with MHC-II antibody using UltraVision LP and AEC chromogen. Note membrane staining of lymphocytes.
  • MHC II (HLA-DQ) Antibody (MA5-12298) in FACS
    MA5-12298_FACS_PBMC_scatter_20130708121416.jpg

    Flow Cytometry with anti-MHC II (HLA-DQ) Monoclonal Antibody [SPV-L3] (MA5-12298) (MA5-12298_FACS_PBMC_scatter_20130708121416.jpg)

    Flow Cytometry with anti-MHC II (HLA-DQ) Monoclonal Antibody [SPV-L3] (MA5-12298)

    Flow cytometry analysis of MHC II in peripheral blood mononuclear cells compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 ul of cell solution was added to each tube at a dilution of 2x10^7 cells/ml, followed by the addition of 50 ul of isotype control and primary antibody (Product # MA5-12298) at a dilution of 2 ug/test. Cells were incubated for 30 min at 4ºC and washed with a cell buffer, followed by incubation with a DyLight 488-conjugated goat anti-mouse IgG (H+L) secondary for 30 min at 4ºC in the dark. FACS analysis was performed using 400 ul of cell buffer.
  • MHC II (HLA-DQ) Antibody (MA5-12298) in FACS
    MA5-12298_FACS_PBMC_20130708121438.jpg

    Flow Cytometry with anti-MHC II (HLA-DQ) Monoclonal Antibody [SPV-L3] (MA5-12298) (MA5-12298_FACS_PBMC_20130708121438.jpg)

    Flow Cytometry with anti-MHC II (HLA-DQ) Monoclonal Antibody [SPV-L3] (MA5-12298)

    Flow cytometry analysis of MHC II in peripheral blood mononuclear cells compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 ul of cell solution was added to each tube at a dilution of 2x10^7 cells/ml, followed by the addition of 50 ul of isotype control and primary antibody (Product # MA5-12298) at a dilution of 2 ug/test. Cells were incubated for 30 min at 4ºC and washed with a cell buffer, followed by incubation with a DyLight 488-conjugated goat anti-mouse IgG (H+L) secondary for 30 min at 4ºC in the dark. FACS analysis was performed using 400 ul of cell buffer.
Publications:
Immunohistochemistry
  Species / Dilution Summary
  Ms / Not Cited
MA5-12298 was used in immunohistochemistry to study the role of neural crest-derived cells in the development of murine thymus

Int Immunol. 2005 May;17(5):549-58.
"Presence and distribution of neural crest-derived cells in the murine developing thymus and their potential for differentiation."
Author(s): Yamazaki H, Sakata E, Yamane T, Yanagisawa A, Abe K, Yamamura K, Hayashi S, Kunisada T
Number of Citations: 6
(See PubMed article )
  Ms / Not Cited
MA5-12298 was used in immunohistochemistry to examine the differentiation of dental mesenchymal cells

Stem Cells. 2007 Jan;25(1):78-87.
"Potential of dental mesenchymal cells in developing teeth."
Author(s): Yamazaki H, Tsuneto M, Yoshino M, Yamamura K, Hayashi S
Number of Citations: 2
(See PubMed article )
Blocking Assay
  Species / Dilution Summary
  Hu / 10 ug/ml
MA5-12298 was used in blocking or activating experiment to investigate the immune response against the p210(BCR-ABL) fusion region in chronic myelogenous leukemia

Cancer Immunol Immunother. 2003 Dec;52(12):761-70.
"Identification of new MHC-restriction elements for presentation of the p210(BCR-ABL) fusion region to human cytotoxic T lymphocytes."
Author(s): Sun JY, Senitzer D, Forman SJ, Chatterjee S, Wong KK Jr
Number of Citations: 1
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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