MEK1 / MAP2K1 Antibody (1C1)

MEK1 / MAP2K1 Monoclonal Antibody for Western blot, IF, IHC (P)

>> See 21 other antibodies for MAP2K1 (MAPKK1; MEK1; MKK1; PRKMK1)
Synonyms:
MAPKK1; MEK1; MKK1; PRKMK1
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgG
Class: Monoclonal
Type: Antibody
Clone: 1C1
Tested Species Reactivity: Human (Hu), Mouse (Ms)
Immunogen: Protein expressed in 293T cell transfected with human MAP2K1 expression vector
Ordering Information
Pierce MEK1 / MAP2K1 Antibody (1C1)
Product #
MA1-095
Size
100 µg
Price
$316.00
Purchase
Add MEK1 / MAP2K1 Antibody (1C1) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:1000
Immunofluorescence (IF) 1:200
Immunohistochemistry (Paraffin) (IHC (P)) 1:10,000-1:100,000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: Protein A
Storage Buffer: PBS with 1mg/ml BSA, 30% glycerol
Preservative: 0.05% sodium azide
Storage Conditions: -20°C
Product Specific Information
MA1-095 has been successfully used with human and mouse samples in Western blot, IHC (P) and immunofluorescence applications.
General Information
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
Product Images
  • MEK1 / MAP2K1 Antibody (MA1-095) in WB
    MA1095 WB.jpg

    Western Blot with anti-MEK1  /  MAP2K1 Monoclonal Antibody [1C1] (MA1-095) (MA1095 WB.jpg)

    Western Blot with anti-MEK1 / MAP2K1 Monoclonal Antibody [1C1] (MA1-095)

    Western blot analysis of MAP2K1 was performed by loading 25µg of A431 and K562 whole cell lysate and 10µg of HEK293T overexpression lysate onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing MAP2K1 (Product #MA1-095) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were then washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Product #31430) at a dilution of 1:15000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Super Signal West Dura (Product #34075).
  • MEK1 / MAP2K1 Antibody (MA1-095) in IF
    MA1095 IF2.JPG

    Immunofluorescence with anti-MEK1  /  MAP2K1 Monoclonal Antibody [1C1] (MA1-095) (MA1095 IF2.JPG)

    Immunofluorescence with anti-MEK1 / MAP2K1 Monoclonal Antibody [1C1] (MA1-095)

    Immunofluorescent analysis of MAP2K1 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 5% normal goat serum (Product #31873) for 15 minutes at room temperature. Cells were then probed with a mouse monoclonal antibody recognizing MAP2K1 (Product# MA1-095), at a dilution of 1:200 for at least 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product# 62249). Images were taken on a Thermo Scientific ArrayScan at 10X magnification.
  • MEK1 / MAP2K1 Antibody (MA1-095) in IF
    MA1-095_IF_Hela.jpg

    Immunofluorescence with anti-MEK1  /  MAP2K1 Monoclonal Antibody [1C1] (MA1-095) (MA1-095_IF_Hela.jpg)

    Immunofluorescence with anti-MEK1 / MAP2K1 Monoclonal Antibody [1C1] (MA1-095)

    Immunofluorescent analysis of MAP2K1 (green) showing positive staining in the cytoplasm of Hela cells (right) compared with a negative control in the absence of primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature and probed with a MAP2K1 monoclonal antibody (Product # MA1-095) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 488-conjugated goat-anti-mouse IgG (H+L) secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with DAPI for 5-10 minutes in the dark. Images were taken at a magnification of 60x.
  • MEK1 / MAP2K1 Antibody (MA1-095) in IF
    MA1-095_IF_NIH-3T3.jpg

    Immunofluorescence with anti-MEK1  /  MAP2K1 Monoclonal Antibody [1C1] (MA1-095) (MA1-095_IF_NIH-3T3.jpg)

    Immunofluorescence with anti-MEK1 / MAP2K1 Monoclonal Antibody [1C1] (MA1-095)

    Immunofluorescent analysis of MAP2K1 (green) showing positive staining in the cytoplasm of NIH-3T3 cells (right) compared with a negative control in the absence of primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature and probed with a MAP2K1 monoclonal antibody (Product # MA1-095) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 488-conjugated goat-anti-mouse IgG (H+L) secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with DAPI for 5-10 minutes in the dark. Images were taken at a magnification of 60x.
  • MEK1 / MAP2K1 Antibody (MA1-095) in IF
    MA1-095_IF_A431.jpg

    Immunofluorescence with anti-MEK1  /  MAP2K1 Monoclonal Antibody [1C1] (MA1-095) (MA1-095_IF_A431.jpg)

    Immunofluorescence with anti-MEK1 / MAP2K1 Monoclonal Antibody [1C1] (MA1-095)

    Immunofluorescent analysis of MAP2K1 (green) showing positive staining in the cytoplasm of A431 cells (right) compared with a negative control in the absence of primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature and probed with a MAP2K1 monoclonal antibody (Product # MA1-095) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 488-conjugated goat-anti-mouse IgG (H+L) secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with DAPI for 5-10 minutes in the dark. Images were taken at a magnification of 60x.
  • MEK1 / MAP2K1 Antibody (MA1-095) in IHC (P)
    MA1-095_IHC_human_cervical_carcinoma_20130731111746.jpg

    Immunohistochemistry (Paraffin) with anti-MEK1  /  MAP2K1 Monoclonal Antibody [1C1] (MA1-095) (MA1-095_IHC_human_cervical_carcinoma_20130731111746.jpg)

    Immunohistochemistry (Paraffin) with anti-MEK1 / MAP2K1 Monoclonal Antibody [1C1] (MA1-095)

    Immunohistochemistry analysis of MAP2K1 showing staining in the nucleus and cytoplasm of paraffin-treated human cervical carcinoma (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a MAP2K1 monoclonal antibody (Product # MA1-095) diluted by 3% BSA-PBS at a dilution of 1:20,000 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
  • MEK1 / MAP2K1 Antibody (MA1-095) in IHC (P)
    MA1-095_IHC_human_bladder_carcinoma_20130731111812.jpg

    Immunohistochemistry (Paraffin) with anti-MEK1  /  MAP2K1 Monoclonal Antibody [1C1] (MA1-095) (MA1-095_IHC_human_bladder_carcinoma_20130731111812.jpg)

    Immunohistochemistry (Paraffin) with anti-MEK1 / MAP2K1 Monoclonal Antibody [1C1] (MA1-095)

    Immunohistochemistry analysis of MAP2K1 showing staining in the nucleus and cytoplasm of paraffin-treated human bladder carcinoma (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a MAP2K1 monoclonal antibody (Product # MA1-095) diluted by 3% BSA-PBS at a dilution of 1:20,000 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
  • MEK1 / MAP2K1 Antibody (MA1-095) in IHC (P)
    MA1-095_IHC_mouse_bladder_tissue_20130731111835.jpg

    Immunohistochemistry (Paraffin) with anti-MEK1  /  MAP2K1 Monoclonal Antibody [1C1] (MA1-095) (MA1-095_IHC_mouse_bladder_tissue_20130731111835.jpg)

    Immunohistochemistry (Paraffin) with anti-MEK1 / MAP2K1 Monoclonal Antibody [1C1] (MA1-095)

    Immunohistochemistry analysis of MAP2K1 showing staining in the nucleus and cytoplasm of paraffin-treated mouse bladder tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a MAP2K1 monoclonal antibody (Product # MA1-095) diluted by 3% BSA-PBS at a dilution of 1:20,000 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific