Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
100 µg (0.25 mg/ml) in PBS with 0.09% (W/V) sodium azide.
Western Blot (WB)
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
PA5-14054 targets MAPK14 in WB applications and shows reactivity with Human samples. The PA5-14054 immunogen is a synthetic peptide surrounding T179 of MAPK14 conjugated to KLH. PA5-14054 detects MAPK14 which has a predicted molecular weight of approximately 41 kDa.
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases, or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
MAPK14 Antibody (PA5-14054) in WB
Western Blot with anti-MAPK14 Polyclonal Antibody (PA5-14054)
Western blot analysis of MAPK14 using anti-MAPK14 polyclonal antibody (PA5-14054) in 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAPK14-pT179.ctrl gene (Lane 2).