Leptin Antibody

Leptin Polyclonal Antibody for Western blot, IF, ICC

>> See 19 other antibodies for LEP (LEP-R, Obesity factor, obesity protein, OB-R, HuB219, CD295 antigen)
Synonyms:
LEP-R, Obesity factor, obesity protein, OB-R, HuB219, CD295 antigen
Entrez Gene ID:
Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu), Mouse (Ms), Porcine (Po)
Published Species Reactivity: Human (Hu), Mouse (Ms), Porcine (Po)
Immunogen: Synthetic peptide corresponding to residues S(91) R N V I Q I S N D L E N L R D(106) of human Leptin.
Ordering Information
Pierce Leptin Antibody
Product #
PA1-052
Size
100 µl
Price
$360.00
Purchase
Add Leptin Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:500-1:5000
Immunofluorescence (IF) 1:20-1:200
Immunocytochemistry (ICC) 1:20-1:200
Published Applications Dilution
Western Blot (WB) See publications below
Immunoprecipitation (IP) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Purification: Ammonium sulfate precipitation
Storage Buffer: PBS
Preservative: 0.05% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA1-052 detects leptin from human, mouse, and porcine samples.

PA1-052 has been successfully used in Western blot and ICC/IF procedures. By Western blot, this antibody detects an ~16 kDa protein representing leptin from human serum.

The PA1-052 immunogen is a synthetic peptide corresponding to residues S(91) R N V I Q I S N D L E N L R D(106) of human Leptin. This sequence is 94% conserved between human and bovine.
General Information
Leptin, the obese (ob) gene product, is a small protein expressed in and secreted from adipose tissue of normal rodents. Studies suggest leptin acts as a circulating hormone capable of regulating body-weight homeostasis and energy balance. One possible target tissue for leptin is the hypothalamus, a proposed control center for satiety and energy expenditure.

Ob knockout mice are characterized by several metabolic abnormalities including hyperglucocorticoidemia, hyperinsulinemia and insulin resistance, hyperglycemia, altered central nervous system activity, reduced metabolic rate of brown adipose tissue, and a large increase in white adipose tissue. Studies show that the administration of recombinant leptin to ob knockout mice reduces food intake and increases energy expenditure.
Product Images
  • Leptin Antibody (PA1-052) in WB
    PA1-052_WB_20131010125159.jpg

    Western Blot with anti-Leptin Polyclonal Antibody (PA1-052) (PA1-052_WB_20131010125159.jpg)

    Western Blot with anti-Leptin Polyclonal Antibody (PA1-052)

    Western blot analysis of Leptin was performed by loading 25 ug of 3T3-L1 (lane 1) and MCF-7 (lane 2) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a Leptin polyclonal antibody (Product # PA1-052) at a dilution of 1:2000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~16kDa.
  • Leptin Antibody (PA1-052) in IF
    PA1-052_IF_Hela_20130724085103.jpg

    Immunofluorescence with anti-Leptin Polyclonal Antibody (PA1-052) (PA1-052_IF_Hela_20130724085103.jpg)

    Immunofluorescence with anti-Leptin Polyclonal Antibody (PA1-052)

    Immunofluorescent analysis of Leptin (green) showing staining in the secretion of HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Leptin polyclonal antibody (Product # PA1-052) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 100x.
  • Leptin Antibody (PA1-052) in IF
    PA1-052_IF_HepG2_20130724085123.jpg

    Immunofluorescence with anti-Leptin Polyclonal Antibody (PA1-052) (PA1-052_IF_HepG2_20130724085123.jpg)

    Immunofluorescence with anti-Leptin Polyclonal Antibody (PA1-052)

    Immunofluorescent analysis of Leptin (green) showing staining in the secretion of HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Leptin polyclonal antibody (Product # PA1-052) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 100x.
  • Leptin Antibody (PA1-052) in IF
    PA1-052_IF_NIH-3T3_20130724085141.jpg

    Immunofluorescence with anti-Leptin Polyclonal Antibody (PA1-052) (PA1-052_IF_NIH-3T3_20130724085141.jpg)

    Immunofluorescence with anti-Leptin Polyclonal Antibody (PA1-052)

    Immunofluorescent analysis of Leptin (green) showing staining in the secretion of NIH-3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Leptin polyclonal antibody (Product # PA1-052) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 100x.
Publications:
Western Blot
  Species / Dilution Summary
  Hu / Not Cited
PA1-052 was used in western blot to study the release of leptin from human skeletal leg muscle in vivo

Cytokine. 2012 Dec;60(3):667-73.
"Human skeletal muscle releases leptin in vivo."
Author(s): Wolsk E, Mygind H, Grøndahl TS, Pedersen BK, van Hall G
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
PA1-052 was used in western blot to investigate the relationship between leptin and body lipid content in mice

Nat Med. 1995 Dec;1(12):1311-4.
"Leptin levels reflect body lipid content in mice: evidence for diet-induced resistance to leptin action."
Author(s): Frederich RC, Hamann A, Anderson S, Löllmann B, Lowell BB, Flier JS
Number of Citations: 74
(See PubMed article )
  Ms / Not Cited
PA1-052 was used in immunoprecipitation and western blot to investigate the regulation of leptin's expression in adipose tissue and 3T3-L1 adipocytes

Proc Natl Acad Sci U S A. 1995 Sep 26;92(20):9034-7.
"Regulated expression of the obese gene product (leptin) in white adipose tissue and 3T3-L1 adipocytes."
Author(s): MacDougald OA, Hwang CS, Fan H, Lane MD
Number of Citations: 34
(See PubMed article )
  Ms / 0
PA1-052 was used in western blot to investigate the role of IL-6 in exercise-induced adipose tissue changes

Acta Physiol (Oxf). 2012 Jun;205(2):224-35.
"IL-6 regulates exercise and training-induced adaptations in subcutaneous adipose tissue in mice."
Author(s): Brandt C, Jakobsen AH, Adser H, Olesen J, Iversen N, Kristensen JM, Hojman P, Wojtaszewski JF, Hidalgo J, Pilegaard H
Number of Citations: 1
(See PubMed article )
  Po / 1:500
PA1-052 was used in western blot to study hypothalamic expression of leptin and long-form leptin-receptor proteins during oestrous cycle and pregnancy

Reprod Domest Anim. 2009 Dec;44(6):920-6.
"Expression of leptin and long-form leptin-receptor proteins in porcine hypothalamus during oestrous cycle and pregnancy."
Author(s): Siawrys G, Kaminski T, Smolinska N, Przala J
Number of Citations: 1
(See PubMed article )
  Po / 1:1000
PA1-052 was used in western blot to investigate the effect of acute fasting on leptin and PPAR-gamma production in pigs

J Anim Physiol Anim Nutr (Berl). 2010 Dec;94(6):e266-76.
"Effects of acute fasting and age on leptin and peroxisome proliferator-activated receptor gamma production relative to fat depot in immature and mature pigs."
Author(s): O'Gorman CW, Stanko RL, Keisler DH, Garcia MR
Number of Citations: 0
(See PubMed article )
Immunoprecipitation
  Species / Dilution Summary
  Ms / Not Cited
PA1-052 was used in immunoprecipitation and western blot to investigate the regulation of leptin's expression in adipose tissue and 3T3-L1 adipocytes

Proc Natl Acad Sci U S A. 1995 Sep 26;92(20):9034-7.
"Regulated expression of the obese gene product (leptin) in white adipose tissue and 3T3-L1 adipocytes."
Author(s): MacDougald OA, Hwang CS, Fan H, Lane MD
Number of Citations: 34
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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