Leptin Antibody

Leptin Polyclonal Antibody for Western blot, ICC, IHC (F)

>> See 19 other antibodies for LEP (LEP-R, Obesity factor, obesity protein, OB-R, HuB219, CD295 antigen)
Synonyms:
LEP-R, Obesity factor, obesity protein, OB-R, HuB219, CD295 antigen
Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt), Ovine (Ov)
Published Species Reactivity: Avian (Av), Human (Hu), Mouse (Ms), Porcine (Po), Rat (Rt)
Immunogen: Synthetic peptide corresponding to residues Q(25) K V Q D D T K T L I K T I V T R I N D(44) of mouse Leptin.
Ordering Information
Pierce Leptin Antibody
Product #
PA1-051
Size
50 µl
Price
$360.00
Purchase
Add Leptin Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:200 - 1:1000
Immunocytochemistry (ICC) 1:50 - 1:200
Immunohistochemistry (Frozen) (IHC (F)) 1:50 - 1:200
Published Applications Dilution
Western Blot (WB) See publications below
Immunocytochemistry (ICC) See publications below
Immunohistochemistry (IHC) See publications below
Blocking Assay (BLOCK) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 2mg/ml
Purification: Antigen affinity chromatography
Storage Buffer: PBS with 1mg/ml BSA
Preservative: 0.05% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA1-051 detects leptin from human, mouse, sheep and rat samples.

PA1-051 has been successfully used in Western blot, immunocytochemistry and immunohistochemistry experiments. By Western blot, this antibody detects an ~16 kDa protein representing leptin from 3T3-L1 cell extract. Immunohistochemical staining of leptin in sheep brain with PA1-051 results in staining of the ventromedial hypothalamus.

The PA1-051 immunogen is a synthetic peptide corresponding to residues Q(25) K V Q D D T K T L I K T I V T R I N D(44) of mouse Leptin. This sequence is completely conserved between mouse and bovine.
General Information
Leptin, the obese (ob) gene product, is a small protein expressed in and secreted from adipose tissue of normal rodents. Studies suggest leptin acts as a circulating hormone capable of regulating body-weight homeostasis and energy balance. One possible target tissue for leptin is the hypothalamus, a proposed control center for satiety and energy expenditure.

Ob gene knockout mice are characterized by several metabolic abnormalities including hyperglucocorticoidemia, hyperinsulinemia and insulin resistance, hyperglycemia, altered central nervous system activity, reduced metabolic rate of brown adipose tissue, and a large increase in white adipose tissue. Studies show that the administration of recombinant leptin to ob knockout mice reduces food intake and increases energy expenditure.
Product Images
  • Leptin Antibody (PA1-051) in WB
    PA1-051_1_07232010.jpg

    Western Blot with anti-Leptin Polyclonal Antibody (PA1-051) (PA1-051_1_07232010.jpg)

    Western Blot with anti-Leptin Polyclonal Antibody (PA1-051)

    Western blot detection of leptin in mouse 3T3-L1 using PA1-051.
  • Leptin Antibody (PA1-051) in IHC
    LEP_Antibody_PA1-051_Immunohistochemistry.jpg

    Immunohistochemistry with anti-Leptin Polyclonal Antibody (PA1-051) (LEP_Antibody_PA1-051_Immunohistochemistry.jpg)

    Immunohistochemistry with anti-Leptin Polyclonal Antibody (PA1-051)

    Immunohistochemical staining of Leptin in sheep brain using PA1-051.
Publications:
Western Blot
  Species / Dilution Summary
  Av / 1:5000
PA1-051 was used in western blot to investigate the localization of leptin expression in the dunlin

Gen Comp Endocrinol. 2006 Sep 15;148(3):336-9.
"Leptin is synthesized in the liver and adipose tissue of the dunlin (Calidris alpina)."
Author(s): Kochan Z, Karbowska J, Meissner W
Number of Citations: 1
(See PubMed article )
  Hu / 1:1333
PA1-051 was used in western blot and western blot to investigate the role of transcription factor AP-2beta in the regulation of leptin secretion

Int J Obes (Lond). 2010 Apr;34(4):670-8.
"Transcription factor AP-2beta inhibits expression and secretion of leptin, an insulin-sensitizing hormone, in 3T3-L1 adipocytes."
Author(s): Fuke T, Yoshizaki T, Kondo M, Morino K, Obata T, Ugi S, Nishio Y, Maeda S, Kashiwagi A, Maegawa H
Number of Citations: 2
(See PubMed article )
  Hu / 1:1000
PA1-051 was used in western blot to investigate the mechanism of leptin down-regulation in 27-hydroxycholesterol-treated human neuroblastoma cells

Cell Signal. 2012 Feb;24(2):484-92.
"Endoplasmic reticulum stress-induced CHOP activation mediates the down-regulation of leptin in human neuroblastoma SH-SY5Y cells treated with the oxysterol 27-hydroxycholesterol."
Author(s): Marwarha G, Dasari B, Ghribi O
Number of Citations: 0
(See PubMed article )
  Ms / 1:1333
PA1-051 was used in western blot and western blot to investigate the role of transcription factor AP-2beta in the regulation of leptin secretion

Int J Obes (Lond). 2010 Apr;34(4):670-8.
"Transcription factor AP-2beta inhibits expression and secretion of leptin, an insulin-sensitizing hormone, in 3T3-L1 adipocytes."
Author(s): Fuke T, Yoshizaki T, Kondo M, Morino K, Obata T, Ugi S, Nishio Y, Maeda S, Kashiwagi A, Maegawa H
Number of Citations: 2
(See PubMed article )
  Ms / 1:1000
PA1-051 was used in western blot to investigate the effect of leptin treatment on histopathology and memory capability in Alzheimer disease model

J Alzheimers Dis. 2010;19(4):1155-67.
"Leptin reduces pathology and improves memory in a transgenic mouse model of Alzheimer's disease."
Author(s): Greco SJ, Bryan KJ, Sarkar S, Zhu X, Smith MA, Ashford JW, Johnston JM, Tezapsidis N, Casadesus G
Number of Citations: 1
(See PubMed article )
  Po / Not Cited
PA1-051 was used in immunohistochemistry and western blot to study the localization of the leptin and the obesity receptor in the small intestine and colon.

J Histochem Cytochem. 2008 Jul;56(7):677-85.
"Leptin and the obesity receptor (OB-R) in the small intestine and colon: a colocalization study."
Author(s): Hansen GH, Niels-Christiansen LL, Danielsen EM
Number of Citations: 1
(See PubMed article )
Immunocytochemistry
  Species / Dilution Summary
  Ms / 1:1,000
Rt / Not Cited
PA1-051 was used in immunocytochemistry and blocking/activating experiment to study the change of leptin immunoreactivity in diabetic rats

Neuroreport. 1999 Feb 5;10(2):437-42.
"Leptin immunoreactivity in the central nervous system in normal and diabetic rats."
Author(s): Li HY, Wang LL, Yeh RS
Number of Citations: 3
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  Po / Not Cited
PA1-051 was used in immunohistochemistry and western blot to study the localization of the leptin and the obesity receptor in the small intestine and colon.

J Histochem Cytochem. 2008 Jul;56(7):677-85.
"Leptin and the obesity receptor (OB-R) in the small intestine and colon: a colocalization study."
Author(s): Hansen GH, Niels-Christiansen LL, Danielsen EM
Number of Citations: 1
(See PubMed article )
Blocking Assay
  Species / Dilution Summary
  Rt / Not Cited
PA1-051 was used in immunocytochemistry and blocking/activating experiment to study the change of leptin immunoreactivity in diabetic rats

Neuroreport. 1999 Feb 5;10(2):437-42.
"Leptin immunoreactivity in the central nervous system in normal and diabetic rats."
Author(s): Li HY, Wang LL, Yeh RS
Number of Citations: 3
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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