200 µg of purified antibody (0.2 mg/ml) in 10mM PBS (p.H. 7.4, with 0.2% BSA and 0.09% sodium azide.
Applications
Dilution *
Immunofluorescence (IF)
Assay Dependent
Immunohistochemistry (Paraffin) (IHC (P))
4 - 8 µg/ml, 30 min @ RT
Flow Cytometry (FACS)
Assay Dependent
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
MA1-38052 detects Laminin Receptor from human samples.
MA1-38052 has been successfully used in immunohistochemistry (paraffin), immunofluorescence, and FACS applications. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, p.H. 6.0 for 10 minutes followed by cooling at room temp for 20 minutes. Positive control of breast carcinoma suggested.
The MA1-38052 immunogen is small cell lung carcinoma N592 live cells.
General Information
Laminin, one of the major glycoproteins of basement membrane, displays multiple biological activities which are mediated through its interaction with specific cell membrane receptors. Overexpression of Laminin Receptor is reportedly an independent prognostic factor in breast cancer and correlates with the dissemination of the tumor cells in bone marrow. Laminin Receptor appears to play an important role in tumor invasion and metastasis.
Product Images
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IHC using MA1-38052
Immunohistochemistry with anti-Laminin Receptor Monoclonal Antibody [MLuC5] (MA1-38052)