Lamin A-C Antibody (mab636)

Lamin A-C Monoclonal Antibody for Western blot, IF, IHC (F)

>> See 16 other antibodies for LMNA (70 kDa lamin)
Synonyms:
70 kDa lamin
UniProt ID:
Details
Host / Isotype: Mouse / IgG2b
Class: Monoclonal
Type: Antibody
Clone: mab636
Tested Species Reactivity: Human (Hu), Bovine (Bv), Porcine (Po)
Published Species Reactivity: Human (Hu), Mouse (Ms), Porcine (Po), Rat (Rt)
Immunogen: Porcine lamin preparation.
Ordering Information
Pierce Lamin A-C Antibody (mab636)
Product #
MA3-1000
Size
200 µl
Price
$199.00
Purchase
Add Lamin A-C Antibody (mab636) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:100 - 1:2000
Immunofluorescence (IF) 1:100
Immunohistochemistry (Frozen) (IHC (F)) 1:100
Published Applications Dilution
Western Blot (WB) See publications below
Immunocytochemistry (ICC) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Storage Buffer: tissue culture supernatant
Preservative: 0.05% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
MA3-1000 detects lamin A/C from human, bovine and porcine samples.

MA3-1000 has been successfully used in Western blot, immunofluorescence and immunohistochemistry procedures. By Western blot, this antibody detects ~ 70 and 65 kDa proteins corresponding to lamin A and C, respectively, from HeLa cell extract. This antibody can also be used for immunofluorescence and immunohistochemistry for subcellular localization experiments. MA3-1000 has been widely used for detection of lamin A/C control in siRNA experiments.

The MA3-1000 antigen is porcine lamin preparation.
General Information
Lamins are a class of intermediate filament proteins that form a matrix on the inner surface of the nuclear envelope. These proteins are found in many different cell types in three different forms (A, B, and C). Lamins A and C are alternatively spliced versions of the LMNA gene. The LMNA gene has been linked to many disorders of the muscular system, nervous system, and the fat distributions systems including: Emery-Dreifuss muscular dystrophy, Dunnigan-type familial partial lipodystrophy (FPLD), limb-girdle muscular dystrophy (LGMD1B), dilated cardiomyopathy (CMD1A), axonal neuropathy (Charcot-Marie-Tooth disease; CMT2B1), and mandibuloacral dysplasia (MAD).
Product Images
  • Lamin A-C Antibody (MA3-1000) in WB
    MA3-1000 WB.jpg

    Western Blot with anti-Lamin A-C Monoclonal Antibody [mab636] (MA3-1000) (MA3-1000 WB.jpg)

    Western Blot with anti-Lamin A-C Monoclonal Antibody [mab636] (MA3-1000)

    Western blot analysis of Lamin A/C was performed by loading 10µg nuclear extract from fractionated mouse tissue lysate onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing phospho-Lamin A/C (Product #MA3-1000) at a dilution of 1:500 overnight at 4°C on a rocking platform. Membranes were then washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Product #31430) at a dilution of 1:15000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Super Signal West Dura (Product #34075).
  • Lamin A-C Antibody (MA3-1000) in WB
    MA3-1000 Outside WB data.jpg

    Western Blot with anti-Lamin A-C Monoclonal Antibody [mab636] (MA3-1000) (MA3-1000 Outside WB data.jpg)

    Western Blot with anti-Lamin A-C Monoclonal Antibody [mab636] (MA3-1000)

    Western blot analysis of Lamin A/C was performed by loading various amounts of Human fibroblast whole cell lysate onto a 10% SDS-PAGE gel. Proteins were transferred to a nitrocellulose membrane and blocked with PBS-0.002% Tween-20 containing 3% non-fat dry milk for at least 1 hour. Membranes were probed with a mouse monoclonal antibody recognizing Lamin A/C (Product #MA3-1000) at a dilution of 1:2000 overnight at 4ºC on a rocking platform. Membranes were then washed in PBS-0.002% Tween-20 and probed with a goat anti-mouse-HRP secondary antibody (Product #32430) at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Super Signal West Pico (Product # 37087).
  • Lamin A-C Antibody (MA3-1000) in IF
    MA3-1000 IF Image Old.jpg

    Immunofluorescence with anti-Lamin A-C Monoclonal Antibody [mab636] (MA3-1000) (MA3-1000 IF Image Old.jpg)

    Immunofluorescence with anti-Lamin A-C Monoclonal Antibody [mab636] (MA3-1000)

    Immunofluorescent analysis of Lamin A/C (red) in HeLa cells at high magnification.
  • Lamin A-C Antibody (MA3-1000) in IF
    MA3-1000 IF Image.jpg

    Immunofluorescence with anti-Lamin A-C Monoclonal Antibody [mab636] (MA3-1000) (MA3-1000 IF Image.jpg)

    Immunofluorescence with anti-Lamin A-C Monoclonal Antibody [mab636] (MA3-1000)

    Immunofluorescent analysis of Lamin A/C (green) in untreated U2-OS cells (A) or HeLa cells (B). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 0.3% BSA for 15 minutes at room temperature. Cells were then probed with a mouse monoclonal antibody recognizing Lamin A/C (Product # MA3-1000), at a dilution of 1:100 for at least 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody (Product# 35552) at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product# 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.
  • Lamin A-C Antibody (MA3-1000) in IF
    MA3-1000-IF1.jpg

    Immunofluorescence with anti-Lamin A-C Monoclonal Antibody [mab636] (MA3-1000) (MA3-1000-IF1.jpg)

    Immunofluorescence with anti-Lamin A-C Monoclonal Antibody [mab636] (MA3-1000)

    Immunofluorescent analysis of Lamin A/C (green) in Human fibroblast cells. Methanol fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 5% normal goat serum (Product #31873) for 15 minutes at room temperature. Cells were then probed with a mouse monoclonal antibody recognizing Lamin A/C (Product# MA3-1000), at a dilution of 1:200 for at least 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody at a dilution of 1:400 for 30 minutes at room temperature.
  • Lamin A-C Antibody (MA3-1000) in IF
    Lamin-A-C_MA3-1000_Immunofluorescence_A549.jpg

    Immunofluorescence with anti-Lamin A-C Monoclonal Antibody [mab636] (MA3-1000) (Lamin-A-C_MA3-1000_Immunofluorescence_A549.jpg)

    Immunofluorescence with anti-Lamin A-C Monoclonal Antibody [mab636] (MA3-1000)

    Immunofluorescent analysis of Lamin A/C using anti-Lamin A/C monoclonal antibody (Product# MA3-1000) shows staining in A549 Cells.
  • Lamin A-C Antibody (MA3-1000) in IF
    Lamin-A-C_MA3-1000_Immunofluorescence_HMVEC.jpg

    Immunofluorescence with anti-Lamin A-C Monoclonal Antibody [mab636] (MA3-1000) (Lamin-A-C_MA3-1000_Immunofluorescence_HMVEC.jpg)

    Immunofluorescence with anti-Lamin A-C Monoclonal Antibody [mab636] (MA3-1000)

    Immunofluorescent analysis of Lamin A/C using anti-Lamin A/C monoclonal antibody (Product# MA3-1000) shows staining in HMVEC Cells.
  • Lamin A-C Antibody (MA3-1000) in IF
    Lamin-A-C_MA3-1000_Immunofluorescence_Hela_Cells.jpg

    Immunofluorescence with anti-Lamin A-C Monoclonal Antibody [mab636] (MA3-1000) (Lamin-A-C_MA3-1000_Immunofluorescence_Hela_Cells.jpg)

    Immunofluorescence with anti-Lamin A-C Monoclonal Antibody [mab636] (MA3-1000)

    Immunofluorescent analysis of Lamin A/C using Lamin A/C Monoclonal Antibody (mab636) (Product# MA3-1000 ) shows staining in Hela Cells. Lamin A/C (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Lamin A/C (Product# MA3-1000 ) at a dilution of 1:20 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
  • Lamin A-C Antibody (MA3-1000) in IF
    Lamin-A-C_MA3-1000_Immunofluorescence_U251_Cells.jpg

    Immunofluorescence with anti-Lamin A-C Monoclonal Antibody [mab636] (MA3-1000) (Lamin-A-C_MA3-1000_Immunofluorescence_U251_Cells.jpg)

    Immunofluorescence with anti-Lamin A-C Monoclonal Antibody [mab636] (MA3-1000)

    Immunofluorescent analysis of Lamin A/C using Lamin A/C Monoclonal Antibody (mab636) (Product# MA3-1000 ) shows staining in U251 Cells. Lamin A/C (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Lamin A/C (Product# MA3-1000 ) at a dilution of 1:200 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
Publications:
Western Blot
  Species / Dilution Summary
  Hu / Not Cited
MA3-1000 was used in western blot and immunocytochemistry to study the effect of thioglycollate on the expression of nuclear lamins A/C

Exp Cell Res. 1990 Oct;190(2):185-94.
"Induction of nuclear lamins A/C in macrophages in in vitro cultures of rat bone marrow precursor cells and human blood monocytes, and in macrophages elicited in vivo by thioglycollate stimulation."
Author(s): Röber RA, Gieseler RK, Peters JH, Weber K, Osborn M
Number of Citations: 3
(See PubMed article )
  Hu / 1:100
MA3-1000 was used in immunocytochemistry and western blot to investigate the effect of 21nt siRNA duplexes for RNAi in mammalian cells

Nature. 2001 May 24;411(6836):494-8.
"Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells."
Author(s): Elbashir SM, Harborth J, Lendeckel W, Yalcin A, Weber K, Tuschl T
Number of Citations: 1
(See PubMed article )
  Hu / 0
MA3-1000 was used in western blot to study the localization and functions of myosin Va and Vb

Cell Motil Cytoskeleton. 2009 Dec;66(12):1057-72.
"Myosin Vb localises to nucleoli and associates with the RNA polymerase I transcription complex."
Author(s): Lindsay AJ, McCaffrey MW
Number of Citations: 7
(See PubMed article )
  Hu / 1:200
MA3-1000 was used in western blot to investigate the effect of RANBP 16 and 17 on bHLH transcription factor E12 function

J Cell Biochem. 2010 Sep 1;111(1):195-206.
"Identification of RANBP16 and RANBP17 as novel interaction partners for the bHLH transcription factor E12."
Author(s): Lee JH, Zhou S, Smas CM
Number of Citations: 1
(See PubMed article )
  Ms / 1:100
MA3-1000 was used in immunocytochemistry and western blot to investigate the effect of 21nt siRNA duplexes for RNAi in mammalian cells

Nature. 2001 May 24;411(6836):494-8.
"Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells."
Author(s): Elbashir SM, Harborth J, Lendeckel W, Yalcin A, Weber K, Tuschl T
Number of Citations: 1
(See PubMed article )
  Po / Not Cited
MA3-1000 was used in western blot and immunocytochemistry to study the effect of thioglycollate on the expression of nuclear lamins A/C

Exp Cell Res. 1990 Oct;190(2):185-94.
"Induction of nuclear lamins A/C in macrophages in in vitro cultures of rat bone marrow precursor cells and human blood monocytes, and in macrophages elicited in vivo by thioglycollate stimulation."
Author(s): Röber RA, Gieseler RK, Peters JH, Weber K, Osborn M
Number of Citations: 3
(See PubMed article )
Immunocytochemistry
  Species / Dilution Summary
  Hu / Not Cited
MA3-1000 was used in immunocytochemistry and western blot to investigate the effect of 21nt siRNA duplexes for RNAi in mammalian cells

Nature. 2001 May 24;411(6836):494-8.
"Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells."
Author(s): Elbashir SM, Harborth J, Lendeckel W, Yalcin A, Weber K, Tuschl T
Number of Citations: 1
(See PubMed article )
  Hu / 0
MA3-1000 was used in immunocytochemistry to study the role of the HIV regulatory protein Vpr in G2 arrest

Virology. 2012 Oct 25;432(2):444-51.
"Perinuclear localization of the HIV-1 regulatory protein Vpr is important for induction of G2-arrest."
Author(s): Sörgel S, Fraedrich K, Votteler J, Thomas M, Stamminger T, Schubert U
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
MA3-1000 was used in immunocytochemistry and western blot to investigate the effect of 21nt siRNA duplexes for RNAi in mammalian cells

Nature. 2001 May 24;411(6836):494-8.
"Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells."
Author(s): Elbashir SM, Harborth J, Lendeckel W, Yalcin A, Weber K, Tuschl T
Number of Citations: 1
(See PubMed article )
  Rt / Not Cited
MA3-1000 was used in western blot and immunocytochemistry to study the effect of thioglycollate on the expression of nuclear lamins A/C

Exp Cell Res. 1990 Oct;190(2):185-94.
"Induction of nuclear lamins A/C in macrophages in in vitro cultures of rat bone marrow precursor cells and human blood monocytes, and in macrophages elicited in vivo by thioglycollate stimulation."
Author(s): Röber RA, Gieseler RK, Peters JH, Weber K, Osborn M
Number of Citations: 3
(See PubMed article )
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