Lactate Dehydrogenase A Antibody

Lactate Dehydrogenase A Polyclonal Antibody for Western blot, IF, ICC, IHC (P)

>> See 7 other antibodies for LDHA (PIG19, GSD11, LDH1, LDHM, L-lactate dehydrogenase A chain, LDH muscle subunit, LDH-A, LDH-M, cell proliferation-inducing gene 19 protein, lactate dehydrogenase M, proliferation-inducing gene 19, renal carcinoma antigen NY-REN-59)
Synonyms:
PIG19, GSD11, LDH1, LDHM, L-lactate dehydrogenase A chain, LDH muscle subunit, LDH-A, LDH-M, cell proliferation-inducing gene 19 protein, lactate dehydrogenase M, proliferation-inducing gene 19, renal carcinoma antigen NY-REN-59
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu), Mouse (Ms)
Immunogen: Synthetic peptide made to an C-terminal portion of the human LDHA protein (within residues 280-332).
Ordering Information
Pierce Lactate Dehydrogenase A Antibody
Product #
PA5-23036
Size
100 ul
Price
$316.00
Purchase
Add Lactate Dehydrogenase A Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:1000-1:2500
Immunofluorescence (IF) 1:200
Immunocytochemistry (ICC) 1:200
Immunohistochemistry (Paraffin) (IHC (P)) 1:100
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1.25mg/ml
Purification: Antigen affinity chromatography
Storage Buffer: PBS with 30% glycerol
Preservative: 0.1% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
This antibody is predicted to react with bovine and porcine based on 100% sequence homology.
General Information
Lactate dehydrogenase (LDH) is an ubiquitous enzyme commonly found in a wide variety of organisms, including plants and microbes. LDH is involved in the interconversion of the pyruvate and NADH to lactate and NAD+. It is also called Hydroxybutyrate Dehydrogenase (HBD), due to the fact that it can catalyze the oxidation of hydroxybutyrate (1). In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C, forming various terameric isoenzymes (140 kDa). Lactate dehydrogenase A (LDH-A, muscle subunit, LDH-M) is involved in the final step of anaerobic glycolysis and catalyzes the conversion of L-lactate and NAD to pryruvate and NADH. While it is predominantly expressed in muscle tissue, it is hormonally regulated in rodents and overexpressed during mammary gland tumorigenesis (2). A mutation that causes deficiency in LDH-A has been implicated in exertional myoglobinuria (3).
Product Images
  • Lactate Dehydrogenase A Antibody (PA5-23036) in WB
    PA5-23036_WesternBlot.jpg

    Western Blot with anti-Lactate Dehydrogenase A Polyclonal Antibody (PA5-23036) (PA5-23036_WesternBlot.jpg)

    Western Blot with anti-Lactate Dehydrogenase A Polyclonal Antibody (PA5-23036)

    Western blot analysis of Lactate Dehydrogenase A (LDHA) was performed on NIH/3T3 whole cell lysates using a Lactate Dehydrogenase A polyclonal antibody (Product # PA5-23036).
  • Lactate Dehydrogenase A Antibody (PA5-23036) in WB
    PA5-23036_WesternBlot_2.jpg

    Western Blot with anti-Lactate Dehydrogenase A Polyclonal Antibody (PA5-23036) (PA5-23036_WesternBlot_2.jpg)

    Western Blot with anti-Lactate Dehydrogenase A Polyclonal Antibody (PA5-23036)

    Western blot analysis of Lactate Dehydrogenase A (LDHA) was performed on HeLa whole cell lysates using a Lactate Dehydrogenase A polyclonal antibody (Product # PA5-23036).
  • Lactate Dehydrogenase A Antibody (PA5-23036) in WB
    Lactate-Dehydrogenase-A-Antibody-PA5-23036-Western-blot-3_20141219093622.jpg

    Western Blot with anti-Lactate Dehydrogenase A Polyclonal Antibody (PA5-23036) (Lactate-Dehydrogenase-A-Antibody-PA5-23036-Western-blot-3_20141219093622.jpg)

    Western Blot with anti-Lactate Dehydrogenase A Polyclonal Antibody (PA5-23036)

    Western blot analysis of Lactate Dehydrogenase A (LDHA) was performed by loading 30ug of normal or diabetic human melanocyte whole cell lysates, treated with 0.4mM palmitate for 0, 4 or 24 hours, per well onto an SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk for 1 hour. The membrane was probed with an LDHA polyclonal antibody (Product # PA5-23036) at a dilution of 1:1000 at 4C overnight, washed in PBST, and probed with an HRP-conjugated anti-rabbit IgG secondary antibody at a dilution of 1:10,000 for 1 hour at room temperature. Detection was performed using a chemiluminescent substrate. Data courtesy of the Innovators Program.
  • Lactate Dehydrogenase A Antibody (PA5-23036) in IF
    PA5-23036_Immunofluorescence.jpg

    Immunofluorescence with anti-Lactate Dehydrogenase A Polyclonal Antibody (PA5-23036) (PA5-23036_Immunofluorescence.jpg)

    Immunofluorescence with anti-Lactate Dehydrogenase A Polyclonal Antibody (PA5-23036)

    Immunofluorescence analysis of Lactate Dehydrogenase A (LDHA) was performed on HeLa whole cell lysates using a Lactate Dehydrogenase A polyclonal antibody (Product # PA5-23036).
  • Lactate Dehydrogenase A Antibody (PA5-23036) in IHC
    PA5-23036_Immunohistochemistry.jpg

    Immunohistochemistry with anti-Lactate Dehydrogenase A Polyclonal Antibody (PA5-23036) (PA5-23036_Immunohistochemistry.jpg)

    Immunohistochemistry with anti-Lactate Dehydrogenase A Polyclonal Antibody (PA5-23036)

    Immunohistochemistry analysis of Lactate Dehydrogenase A (LDHA) was performed lung tissue using a Lactate Dehydrogenase A polyclonal antibody (Product # PA5-23036).
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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