* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Antigen affinity chromatography
0.1% sodium azide
-20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
This antibody is predicted to react with bovine and porcine based on 100% sequence homology.
Lactate dehydrogenase (LDH) is an ubiquitous enzyme commonly found in a wide variety of organisms, including plants and microbes. LDH is involved in the interconversion of the pyruvate and NADH to lactate and NAD+. It is also called Hydroxybutyrate Dehydrogenase (HBD), due to the fact that it can catalyze the oxidation of hydroxybutyrate (1). In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C, forming various terameric isoenzymes (140 kDa). Lactate dehydrogenase A (LDH-A, muscle subunit, LDH-M) is involved in the final step of anaerobic glycolysis and catalyzes the conversion of L-lactate and NAD to pryruvate and NADH. While it is predominantly expressed in muscle tissue, it is hormonally regulated in rodents and overexpressed during mammary gland tumorigenesis (2). A mutation that causes deficiency in LDH-A has been implicated in exertional myoglobinuria (3).
Lactate Dehydrogenase A Antibody (PA5-23036) in WB PA5-23036_WesternBlot.jpg
Western Blot with anti-Lactate Dehydrogenase A Polyclonal Antibody (PA5-23036)
Western blot analysis of Lactate Dehydrogenase A (LDHA) was performed by loading 30ug of normal or diabetic human melanocyte whole cell lysates, treated with 0.4mM palmitate for 0, 4 or 24 hours, per well onto an SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk for 1 hour. The membrane was probed with an LDHA polyclonal antibody (Product # PA5-23036) at a dilution of 1:1000 at 4C overnight, washed in PBST, and probed with an HRP-conjugated anti-rabbit IgG secondary antibody at a dilution of 1:10,000 for 1 hour at room temperature. Detection was performed using a chemiluminescent substrate. Data courtesy of the Innovators Program.
Lactate Dehydrogenase A Antibody (PA5-23036) in IF PA5-23036_Immunofluorescence.jpg
Immunofluorescence with anti-Lactate Dehydrogenase A Polyclonal Antibody (PA5-23036)