86 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 80 kDa subunit; CTC box-binding factor 85 kDa subunit; CTC85; CTCBF; DNA repair protein XRCC5; Ku autoantigen, 80kDa; Ku86 autoantigen related protein 1; OTTHUMP00000164061; OTTHUMP00000206791; TLAA; X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining; Ku autoantigen, 80kD); X-ray repair cross-complementing protein 5; X-ray repair, complementing de
100 ul of antibody in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 ug/ml BSA and 50% glycerol.
Flow Cytometry (FACS)
Immunohistochemistry (Paraffin) (IHC (P))
Western Blot (WB)
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
PA5-17454 detects Ku80 in human and non-human primate samples. Predicted to react with mouse and rat based on sequence homology.
PA5-17454 has been successfully used in flow cytometry, immunocytochemistry, immunofluorescence, immunohistochemistry (paraffin), immunoprecipitation and Western blot applications.
The PA5-17454 immunogen is synthetic peptide corresponding to the carboxy terminus of human Ku80.
PA5-17454 detects Ku80 which has a predicted molecular weight of approximately 86 kDa.
The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.
Ku80 Antibody (PA5-17454) in IF
Immunofluorescence with anti-Ku80 Polyclonal Antibody (PA5-17454)