Ku80 Antibody
Ku80 Polyclonal Antibody for FACS, ICC, IHC (P), IP, Western blot
Synonyms:
86 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 80 kDa subunit; CTC box-binding factor 85 kDa subunit; CTC85; CTCBF; DNA repair protein XRCC5; Ku autoantigen, 80kDa; Ku86 autoantigen related protein 1; OTTHUMP00000164061; OTTHUMP00000206791; TLAA; X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining; Ku autoantigen, 80kD); X-ray repair cross-complementing protein 5; X-ray repair, complementing de
Entrez Gene ID:
UniProt ID:
(Hu) P13010, (Ms) P27641
| Details | |
| Host / Isotype: | Rabbit |
| Class: | Polyclonal |
| Type: | Antibody |
| Species Reactivity: | Human (Hu) Mouse (Ms) Rat (Rt) Non-human primate (Nhp) |
| Immunogen: | Synthetic peptide corresponding to the carboxy terminus of human Ku80. |
| Ordering Information | ||||
| Pierce Ku80 Antibody |
| Storage: | -20°C |
| Form: | 100 ul of antibody in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 ug/ml BSA and 50% glycerol. |
| Applications | Dilution * |
| Flow Cytometry (FACS) | 1:50 |
| Immunocytochemistry (ICC) | 1:400 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:300 |
| Immunoprecipitation (IP) | 1:25 |
| Western Blot (WB) | 1:1000 |
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* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
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| Product Specific Information |
| PA5-17454 detects Ku80 in human and non-human primate samples. Predicted to react with mouse and rat based on sequence homology. PA5-17454 has been successfully used in flow cytometry, immunocytochemistry, immunofluorescence, immunohistochemistry (paraffin), immunoprecipitation and Western blot applications. The PA5-17454 immunogen is synthetic peptide corresponding to the carboxy terminus of human Ku80. PA5-17454 detects Ku80 which has a predicted molecular weight of approximately 86 kDa. |
| General Information |
| The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. |
| Product Images |
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Ku80 Antibody (PA5-17454) in IF
Immunofluorescence with anti-Ku80 Polyclonal Antibody (PA5-17454)
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Ku80 Antibody (PA5-17454) in IF
Immunofluorescence with anti-Ku80 Polyclonal Antibody (PA5-17454)
Immunofluorescent staining of paraformaldehyde-fixed HeLa cells, using Ku80 polyclonal antibody (Product # PA5-17454). -
Ku80 Antibody (PA5-17454) in FACS
Flow Cytometry with anti-Ku80 Polyclonal Antibody (PA5-17454)
Flow cytometric analysis of Ku80 in HeLa cells using a Ku80 polyclonal antibody (Product # PA5-17454) (blue) compared to a nonspecific negative control antibody (red). -
Ku80 Antibody (PA5-17454) in IHC
Immunohistochemistry with anti-Ku80 Polyclonal Antibody (PA5-17454)
Immunohistochemical analysis of Ku80 in paraffin-embedded human lung carcinoma using a Ku80 polyclonal antibody (Product # PA5-17454) showing nuclear localiztion. -
Ku80 Antibody (PA5-17454) in WB
Western Blot with anti-Ku80 Polyclonal Antibody (PA5-17454)
Western blot analysis of Ku80 in lysates from HeLa and A673 cells using Ku80 polyclonal antibody (Product # PA5-17454).
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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Ku80 Antibodies > Ku80 Polyclonal Antibody PA5-17454
