* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Antigen affinity chromatography
PBS, pH 7,
0.025% Proclin 300
-20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA5-27234 targets K-Ras in IF, IHC (P), ELISA, IP and WB applications and shows reactivity with Human samples.
The PA5-27234 immunogen is synthetic peptide corresponding to a region within amino acids 1 and 73 of Human K-Ras.
This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene.
K-Ras Antibody (PA5-27234) in WB K-Ras_PA5-27234_Antibody_WesternBlot.jpg
Western Blot with anti-K-Ras Polyclonal Antibody (PA5-27234)