|Host / Isotype:||Rabbit / IgG|
|Tested Species Reactivity:||Human (Hu)|
|Immunogen:||Synthetic peptide corresponding to a region within amino acids 1 and 73 of Human K-Ras|
|Pierce K-Ras Antibody|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Immunoprecipitation (IP)||Assay Dependent|
|ELISA (ELISA)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
|Purification:||Antigen affinity chromatography|
|Storage Buffer:||PBS, pH 7, with 20% glycerol|
|Storage Conditions:||-20° C, Avoid Freeze/Thaw Cycles|
|Product Specific Information|
|PA5-27234 targets K-Ras in IF, IHC (P), ELISA, IP and WB applications and shows reactivity with Human samples.
The PA5-27234 immunogen is synthetic peptide corresponding to a region within amino acids 1 and 73 of Human K-Ras.
|This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region.|