Insulin Antibody (INS04 (E2-E3))

Insulin Monoclonal Antibody for IHC (P)

>> See 39 other antibodies for INS (proinsulin)
Synonyms:
proinsulin
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgG1, kappa
Class: Monoclonal
Type: Antibody
Clone: INS04 (E2-E3)
Tested Species Reactivity: Human (Hu)
Published Species Reactivity: Mouse (Ms)
Immunogen: Swine insulin
Ordering Information
Pierce Insulin Antibody (INS04 (E2-E3))
Product #
MA5-12032
Size
500 µl
Price
$215.00
Purchase
Add Insulin Antibody (INS04 (E2-E3)) to your cart.
Tested Applications Dilution *
Immunohistochemistry (Paraffin) (IHC (P)) 1:25-1:50
Published Applications Dilution
Immunohistochemistry (IHC) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 0.2mg/ml
Purification: Protein G
Storage Buffer: PBS, pH 7.4, with 0.2% BSA
Preservative: 0.09% sodium azide
Storage Conditions: 4° C
Product Specific Information
MA5-12032 targets Insulin in immunohistochemistry (paraffin) applications and shows reactivity with human samples.

The MA5-12032 immunogen is swine insulin.
General Information
Insulin is a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as beta-cell of islets of Langerhans and tumor (insulinoma) marker.
Product Images
  • Insulin Antibody (MA5-12032) in IHC
    MA5-12032_Immunohistochemistry.jpg

    Immunohistochemistry with anti-Insulin Monoclonal Antibody [INS04 (E2-E3)] (MA5-12032) (MA5-12032_Immunohistochemistry.jpg)

    Immunohistochemistry with anti-Insulin Monoclonal Antibody [INS04 (E2-E3)] (MA5-12032)

    Formalin-fixed, paraffin-embedded human pancreas stained with Insulin antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of beta cells of the islets of Langerhans.
Publications:
Immunohistochemistry
  Species / Dilution Summary
  Ms / 1:400
MA5-12032 was used in immunohistochemistry to study mitochondrial and cytosolic oxidative stress in streptozotocin-diabetic mouse pancreas and the effect of combined antioxidant therapy

Exp Toxicol Pathol. 2013 Mar;65(3):255-62.
"The potential role of combined antioxidant treatment on pancreas of STZ-diabetic mice."
Author(s): Karatug A, Bolkent S
Number of Citations: 1
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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