* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Antigen affinity chromatography
0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Product Specific Information
IL12B is a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children.
IL12B Antibody (PA5-23965) in WB IL12B-_Antibody_PA5-23965_WB_1_112013.jpg
Western Blot with anti-IL12B Polyclonal Antibody (PA5-23965)
Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human tonsil tissue using an IL12B polyclonal antibody (Product ## PA5-23965), followed by HRP-conjugated secondary antibody and DAB staining.
IL12B Antibody (PA5-23965) in FACS IL12B-_Antibody_PA5-23965_FC_1_112013.jpg
Flow Cytometry with anti-IL12B Polyclonal Antibody (PA5-23965)
Flow cytometry analysis of MDA-MB435 cells using an IL12B polyclonal antibody (Product # PA5-23965) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
(This product is for In Vitro experimental use only. Not for resale without express authorization.)