IL12B Antibody

IL12B Polyclonal Antibody for Western blot, IHC, FACS

>> See 14 other antibodies for IL12B (CLMF, CLMF2, IL-12B, NKSF, NKSF2, CLMF p40, IL-12 subunit p40, IL12, subunit p40, NK cell stimulatory factor chain 2, cytotoxic lymphocyte maturation factor 40 kDa subunit, interleukin 12, p40, interleukin-12 beta chain, interleukin-12 subunit beta, natural killer cell stimulatory factor, 40 kD s)
Synonyms:
CLMF, CLMF2, IL-12B, NKSF, NKSF2, CLMF p40, IL-12 subunit p40, IL12, subunit p40, NK cell stimulatory factor chain 2, cytotoxic lymphocyte maturation factor 40 kDa subunit, interleukin 12, p40, interleukin-12 beta chain, interleukin-12 subunit beta, natural killer cell stimulatory factor, 40 kD s
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit / IgG
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu)
Immunogen: KLH conjugated synthetic peptide between 264-290 amino acids from the C-terminal region of human IL12B
Ordering Information
Pierce IL12B Antibody
Product #
PA5-23965
Size
400 ul
Price
$316.00
Purchase
Add IL12B Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:1000
Immunohistochemistry (IHC) 1:50-1:100
Flow Cytometry (FACS) 1:10-1:50
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: Lot-specific
Purification: Antigen affinity chromatography
Storage Buffer: PBS
Preservative: 0.09% sodium azide
Storage Conditions: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Product Specific Information
General Information
IL12B is a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children.
Product Images
  • IL12B Antibody (PA5-23965) in WB
    IL12B-_Antibody_PA5-23965_WB_1_112013.jpg

    Western Blot with anti-IL12B Polyclonal Antibody (PA5-23965) (IL12B-_Antibody_PA5-23965_WB_1_112013.jpg)

    Western Blot with anti-IL12B Polyclonal Antibody (PA5-23965)

    Western blot analysis using an IL12B polyclonal antibody (Product # PA5-23965) in CEM, MDA-MB453 cell lysates (35ug per lane).
  • IL12B Antibody (PA5-23965) in IHC
    IL12B-_Antibody_PA5-23965_IHC_1_112013.jpg

    Immunohistochemistry with anti-IL12B Polyclonal Antibody (PA5-23965) (IL12B-_Antibody_PA5-23965_IHC_1_112013.jpg)

    Immunohistochemistry with anti-IL12B Polyclonal Antibody (PA5-23965)

    Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human tonsil tissue using an IL12B polyclonal antibody (Product # PA5-23965), followed by HRP-conjugated secondary antibody and DAB staining.
  • IL12B Antibody (PA5-23965) in FACS
    IL12B-_Antibody_PA5-23965_FC_1_112013.jpg

    Flow Cytometry with anti-IL12B Polyclonal Antibody (PA5-23965) (IL12B-_Antibody_PA5-23965_FC_1_112013.jpg)

    Flow Cytometry with anti-IL12B Polyclonal Antibody (PA5-23965)

    Flow cytometry analysis of MDA-MB435 cells using an IL12B polyclonal antibody (Product # PA5-23965) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific