IL-8 Antibody (3IL8-H10)

IL-8 Monoclonal Antibody for Western blot, ELISA

>> See 12 other antibodies for IL8 (C-X-C motif chemokine 8; T cell chemotactic factor; T-cell chemotactic factor; beta-thromboglobulin-like protein; chemokine (C-X-C motif) ligand 8; emoctakin; granulocyte chemotactic protein 1; interleukin-8; lymphocyte-derived neutrophil-activating factor; monocyte-derived neutrophil chemotactic factor; monocyte-derived neutrophil-activating peptide; neutrophil-activating peptide 1; small inducible cytokine subfamily B, member 8)
Synonyms:
C-X-C motif chemokine 8; T cell chemotactic factor; T-cell chemotactic factor; beta-thromboglobulin-like protein; chemokine (C-X-C motif) ligand 8; emoctakin; granulocyte chemotactic protein 1; interleukin-8; lymphocyte-derived neutrophil-activating factor; monocyte-derived neutrophil chemotactic factor; monocyte-derived neutrophil-activating peptide; neutrophil-activating peptide 1; small inducible cytokine subfamily B, member 8
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgG
Class: Monoclonal
Type: Antibody
Clone: 3IL8-H10
Tested Species Reactivity: Human (Hu)
Published Species Reactivity: Human (Hu), Mouse (Ms)
Immunogen: Recombinant human IL-8
Ordering Information
Pierce IL-8 Antibody (3IL8-H10)
Product #
M801
Size
500 ug
Price
$345.00
Purchase
Add IL-8 Antibody (3IL8-H10) to your cart.
Tested Applications Dilution *
Western Blot (WB) Assay Dependent
ELISA (ELISA) Assay Dependent
Published Applications Dilution
Immunohistochemistry (IHC) See publications below
ELISA (ELISA) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1.08mg/ml
Purification: Protein A
Storage Buffer: PBS
Preservative: no preservative
Storage Conditions: -20°C
Product Specific Information
M801 targets IL-8 in ELISA, and WB applications and shows reactivity with Human samples.

The M801 immunogen is recombinant human IL-8.

M801 detects IL-8 which has a predicted molecular weight of approximately 9 kDa.

The M801 IL8 antibody (clone 3IL8-H10) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M802B (biotinylated conjugate of clone I8-S2). Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection.

Antibody M801 (clone 3IL8-H10) and biotinylated antibody M802B (clone I8-S2) have successfully been used in combination with recombinant IL8 protein SIL8 in ELISA applications.
General Information
The protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q.
Product Images
  • IL-8 Antibody (M801) in ELISA
    IL-8_Antibody_M801_ELISA_1_20130515075832.JPG

    ELISA with anti-IL-8 Monoclonal Antibody [3IL8-H10] (M801) (IL-8_Antibody_M801_ELISA_1_20130515075832.JPG)

    ELISA with anti-IL-8 Monoclonal Antibody [3IL8-H10] (M801)

    Sandwich ELISA analysis of human IL-8 was performed using a Human IL-8 Colorimetric ELISA kit (Product # EH2IL8) by loading 50 ul per well of Human IL-8 Recombinant Protein (Product # SIL8) in dodecuplicate at 1000, 400, 160, 64, 25.6, and 0 pg/ml across a 3 ug/ml mouse anti-Human IL-8 (Product # M801) pre-coated plate and incubating for 1 hour at room temperature. The plate was washed, and then incubated with 50 ul per well of a biotinylated mouse anti-human IL-8 monoclonal antibody (Product # M802B) in duplicate at 0.25 ug/ml for 1 hour at room temperature. The plate was washed and incubated with 100 ul per well of Streptavidin-HRP (Product # N504) in all test wells at a 1:4,000 dilution for 30 minutes at room temperature. Detection was performed using 1-Step Ultra TMB substrate (Product # 34028) for 30 minutes at room temperature in the dark. The plate was then stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550 nm.
Publications:
Immunohistochemistry
  Species / Dilution Summary
  Hu / Not Cited
M801 was used in immunohistochemistry to investigate the effects of rapamycin on KC/MIP-2, granzyme B, and interferon gamma expression on cardiac allografts

J Immunol. 1993 Jul 15;151(2):1158-66.
"Rapamycin treatment depresses intragraft expression of KC/MIP-2, granzyme B, and IFN-gamma in rat recipients of cardiac allografts."
Author(s): Wieder KJ, Hancock WW, Schmidbauer G, Corpier CL, Wieder I, Kobzik L, Strom TB, Kupiec-Weglinski JW
Number of Citations: 2
(See PubMed article )
ELISA
  Species / Dilution Summary
  Hu / Not Cited
M801 was used in ELISA to investigate the influence of monocyte Fc gamma receptor cross-linking on the expression of interleukin 8

J Immunol. 1995 Sep 15;155(6):3161-7.
"Monocyte Fc gamma receptor cross-linking induces IL-8 production."
Author(s): Marsh CB, Gadek JE, Kindt GC, Moore SA, Wewers MD
Number of Citations: 11
(See PubMed article )
  Hu / Not Cited
M801 was used in ELISA to investigate the important roles of epithelial cells in inflammatory response to chlamydia

J Clin Invest. 1997 Jan 1;99(1):77-87.
"Secretion of proinflammatory cytokines by epithelial cells in response to Chlamydia infection suggests a central role for epithelial cells in chlamydial pathogenesis."
Author(s): Rasmussen SJ, Eckmann L, Quayle AJ, Shen L, Zhang YX, Anderson DJ, Fierer J, Stephens RS, Kagnoff MF
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
M801 was used in ELISA to investigate the role of surface proteinase-3, alpha1-antitrypsin, and Fcgamma receptors in monocyte interleukin 8 release

J Clin Invest. 1997 Sep 15;100(6):1416-24.
"Antineutrophil cytoplasmic antibodies induce monocyte IL-8 release. Role of surface proteinase-3, alpha1-antitrypsin, and Fcgamma receptors."
Author(s): Ralston DR, Marsh CB, Lowe MP, Wewers MD
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
M801 was used in ELISA to investigate the regulation of interleukin 8 expression in melanoma cells

Cancer Res. 1998 Apr 1;58(7):1532-7.
"Regulation of interleukin 8 expression in human malignant melanoma cells."
Author(s): Singh RK, Varney ML
Number of Citations: 8
(See PubMed article )
  Hu / Not Cited
M801 was used in ELISA to investigate the effect of immunosuppressive regimens on cytokine expression in lung transplanted patients

Transplant Proc. 2003 Jun;35(4):1523-6.
"Monocyte chemoattractant protein-1 levels in bronchoalveolar lavage fluid of lung-transplanted patients treated with tacrolimus as rescue treatment for refractory acute rejection."
Author(s): Meloni F, Cascina A, Paschetto E, Marone Bianco A, Morosini M, Pellegrini C, Fietta A, Vitulo P, Pozzi E, Viganò M
Number of Citations: 1
(See PubMed article )
  Hu / 50-300 ug/ml
M801 was used in ELISA to develop protein microarray technology to monitor biomarkers of rheumatoid arthritis disease

Cell Biol Toxicol. 2003 Jun;19(3):189-202.
"Development of protein microarray technology to monitor biomarkers of rheumatoid arthritis disease."
Author(s): Urbanowska T, Mangialaio S, Hartmann C, Legay F
Number of Citations: 1
(See PubMed article )
  Hu / 1 ug ml
M801 was used in ELISA to study the role of oxidized ATP in proinflammatory signaling

Br J Pharmacol. 2003 Oct;140(3):507-19.
"Oxidized ATP (oATP) attenuates proinflammatory signaling via P2 receptor-independent mechanisms."
Author(s): Beigi RD, Kertesy SB, Aquilina G, Dubyak GR
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
M801 was used in ELISA to investigate the role of CD200R in regulating myeloid cell function

J Immunol. 2006 Jan 1;176(1):191-9.
"Regulation of myeloid cell function through the CD200 receptor."
Author(s): Jenmalm MC, Cherwinski H, Bowman EP, Phillips JH, Sedgwick JD
Number of Citations: 11
(See PubMed article )
  Hu / Not Cited
M801 was used in ELISA to study the role of mitochondrial pathway in neutrophil survival

J Immunol. 2007 Feb 15;178(4):2535-41.
"IgE modulates neutrophil survival in asthma: role of mitochondrial pathway."
Author(s): Saffar AS, Alphonse MP, Shan L, Hayglass KT, Simons FE, Gounni AS
Number of Citations: 4
(See PubMed article )
  Hu / 1:2,000
M801 was used in ELISA to assess the interleukin-8 level in patients with decompensated congestive heart failure

Nephrol Dial Transplant. 2007 Jul;22(7):2013-9.
"Intermittent haemodiafiltration in refractory congestive heart failure: BNP and balance of inflammatory cytokines."
Author(s): Libetta C, Sepe V, Zucchi M, Pisacco P, Cosmai L, Meloni F, Campana C, Rampino T, Monti C, Tavazzi L, Dal Canton A
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
M801 was used in ELISA to investigate the role of p38 kinase in rheumatoid arthritis synovial membranes

Arthritis Rheum. 2010 Nov;62(11):3221-31.
"Inhibitors of p38 suppress cytokine production in rheumatoid arthritis synovial membranes: does variable inhibition of interleukin-6 production limit effectiveness in vivo?"
Author(s): Page TH, Brown A, Timms EM, Foxwell BM, Ray KP
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
M801 was used in ELISA to investigate the effect of transforming growth factor-beta on glucocorticoid activity in human A549 cells

Br J Pharmacol. 2012 Aug;166(7):2036-48.
"Transforming growth factor-? impairs glucocorticoid activity in the A549 lung adenocarcinoma cell line."
Author(s): Salem S, Harris T, Mok JS, Li MY, Keenan CR, Schuliga MJ, Stewart AG
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
M801 was used in ELISA to investigate the role of CD200R in regulating myeloid cell function

J Immunol. 2006 Jan 1;176(1):191-9.
"Regulation of myeloid cell function through the CD200 receptor."
Author(s): Jenmalm MC, Cherwinski H, Bowman EP, Phillips JH, Sedgwick JD
Number of Citations: 11
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific