IL-6 Antibody (5IL6)

IL-6 Monoclonal Antibody for Western blot, ELISA

>> See 20 other antibodies for IL6 (B-cell hybridoma growth factor; interleukin HP-1; interleukin-6)
Synonyms:
B-cell hybridoma growth factor; interleukin HP-1; interleukin-6
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgG1, kappa
Class: Monoclonal
Type: Antibody
Clone: 5IL6
Tested Species Reactivity: Human (Hu)
Published Species Reactivity: Human (Hu), Mouse (Ms)
Immunogen: Recombinant human IL-6.
Ordering Information
Pierce IL-6 Antibody (5IL6)
Product #
M620
Size
500 µg
Price
$350.00
Purchase
Add IL-6 Antibody (5IL6) to your cart.
Tested Applications Dilution *
Western Blot (WB) Assay Dependent
ELISA (ELISA) Assay Dependent
Published Applications Dilution
Immunocytochemistry (ICC) See publications below
ELISA (ELISA) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1.01mg/ml
Purification: Protein G
Storage Buffer: PBS
Preservative: no preservative
Storage Conditions: -20°C
Product Specific Information
M620 targets IL-6 in ELISA, and Western blot applications and shows reactivity with human samples.

The M620 immunogen is recombinant human IL-6.

M620 detects IL-6 which has a predicted molecular weight of approximately 22 kDa.

The M620 IL6 antibody (clone 5IL6) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M621B (biotinylated conjugate of clone 7IL6). Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection.

Antibody M620 (clone 5IL6) and biotinylated antibody M621B (clone 7IL6) have successfully been used in combination with recombinant IL6 protein SIL6 in ELISA applications.
General Information
This gene encodes a cytokine that functions in inflammation and the maturation of B cells. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis.
Product Images
  • IL-6 Antibody (M620) in ELISA
    IL-6_Antibody_M620_ELISA_1_20130515075429.jpg

    ELISA with anti-IL-6 Monoclonal Antibody [5IL6] (M620) (IL-6_Antibody_M620_ELISA_1_20130515075429.jpg)

    ELISA with anti-IL-6 Monoclonal Antibody [5IL6] (M620)

    Sandwich ELISA analysis of human IL-6 was performed using a Human IL-6 Colorimetric ELISA kit (Product # EH2IL6) by loading 50 ul per well of a mouse anti-human IL-6 biotinylated antibody (Product # M621B) at a concentration of 0.035 ug/ml and 50 ul of Human IL-6 Recombinant Protein (Product # SIL6) at 400, 160, 64, 25.6, 10.24, and 0 pg/ml (both in quadruplicate) across a 4 ug/ml mouse anti-human IL-6 (Product # M620) pre-coated plate. The plate was incubated for 2 hours at room temperature, washed, and incubated with 100 ul per well of Streptavidin-HRP (Product # N504) in all test wells at 1:7000 for 30 minutes at room temperature. Detection was performed using 1-Step Ultra TMB Substrate (Product # 34028) for 30 minutes at room temperature in the dark. The plate was stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550 nm.
Publications:
Immunocytochemistry
  Species / Dilution Summary
  Hu / Not Cited
M620 was used in immunocytochemistry to characterize a potent nonsteroidal antagonist against glucocorticoid receptor

Mol Endocrinol. 2003 Jan;17(1):117-27.
"A nonsteroidal glucocorticoid receptor antagonist."
Author(s): Miner JN, Tyree C, Hu J, Berger E, Marschke K, Nakane M, Coghlan MJ, Clemm D, Lane B, Rosen J
Number of Citations: 4
(See PubMed article )
ELISA
  Species / Dilution Summary
  Hu / Not Cited
M620 was used in ELISA to investigate the changes of interleukin 6 level in patients with cancer immunotherapy

Blood. 1994 Sep 15;84(6):1887-95.
"Sustained high levels of circulating chaperoned interleukin-6 after active specific cancer immunotherapy."
Author(s): May LT, Patel K, García D, Ndubuisi MI, Ferrone S, Mittelman A, Mackiewicz A, Sehgal PB
Number of Citations: 3
(See PubMed article )
  Hu / Not Cited
M620 was used in ELISA to assess the interferon-alpha production in peripheral blood mononuclear cell

J Exp Med. 1997 Jun 2;185(11):1987-95.
"Regulation of interleukin-12 by complement receptor 3 signaling."
Author(s): Marth T, Kelsall BL
Number of Citations: 42
(See PubMed article )
  Hu / Not Cited
M620 was used in ELISA to investigate the role of mast cells/basophils in dengue pathogenesis

J Virol. 2000 Aug;74(15):7146-50.
"Release of vasoactive cytokines by antibody-enhanced dengue virus infection of a human mast cell/basophil line."
Author(s): King CA, Marshall JS, Alshurafa H, Anderson R
Number of Citations: 7
(See PubMed article )
  Hu / Not Cited
M620 was used in ELISA to investigate the relationship between serum cytokine levels, cigarette smoking and airway responsiveness among pregnant women

Int Arch Allergy Immunol. 2003 Feb;130(2):158-64.
"Serum cytokine levels, cigarette smoking and airway responsiveness among pregnant women."
Author(s): Tsunoda M, Litonjua AA, Kuniak MP, Weiss ST, Satoh T, Guevarra L, Tollerud DJ
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
M620 was used in ELISA to study the effect of etanercept treatment on proinflammatory cytokine-secreting peripheral blood mononuclear cells in rheumatoid arthritic patients

Rheumatology (Oxford). 2004 Aug;43(8):960-4.
"Long-term treatment with etanercept significantly reduces the number of proinflammatory cytokine-secreting peripheral blood mononuclear cells in patients with rheumatoid arthritis."
Author(s): Schotte H, Schlüter B, Willeke P, Mickholz E, Schorat MA, Domschke W, Gaubitz M
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
M620 was used in ELISA to study the role of mitochondrial pathway in neutrophil survival

J Immunol. 2007 Feb 15;178(4):2535-41.
"IgE modulates neutrophil survival in asthma: role of mitochondrial pathway."
Author(s): Saffar AS, Alphonse MP, Shan L, Hayglass KT, Simons FE, Gounni AS
Number of Citations: 4
(See PubMed article )
  Hu / Not Cited
M620 was used in ELISA to study the role of human mast cells in the recruitment of natural killer cells

Blood. 2008 Jun 15;111(12):5467-76.
"Human mast cell activation with virus-associated stimuli leads to the selective chemotaxis of natural killer cells by a CXCL8-dependent mechanism."
Author(s): Burke SM, Issekutz TB, Mohan K, Lee PW, Shmulevitz M, Marshall JS
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
M620 was used in ELISA to investigate the role of p38 kinase in rheumatoid arthritis synovial membranes

Arthritis Rheum. 2010 Nov;62(11):3221-31.
"Inhibitors of p38 suppress cytokine production in rheumatoid arthritis synovial membranes: does variable inhibition of interleukin-6 production limit effectiveness in vivo?"
Author(s): Page TH, Brown A, Timms EM, Foxwell BM, Ray KP
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
M620 was used in ELISA to study the effect of Lactobacillus casei strain Shirota on the production of interleukin 6 in LPS-stimulated murine large intestinal lamina propria mononuclear cells

Clin Exp Immunol. 2005 Jun;140(3):417-26.
"Probiotic Lactobacillus-induced improvement in murine chronic inflammatory bowel disease is associated with the down-regulation of pro-inflammatory cytokines in lamina propria mononuclear cells."
Author(s): Matsumoto S, Hara T, Hori T, Mitsuyama K, Nagaoka M, Tomiyasu N, Suzuki A, Sata M
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
M620 was used in ELISA to investigate the role of Lactobacillus polysaccharide peptidoglycan complex in chronic intestinal inflammatory disorders

Immunology. 2009 Sep;128(1 Suppl):e170-80.
"A component of polysaccharide peptidoglycan complex on Lactobacillus induced an improvement of murine model of inflammatory bowel disease and colitis-associated cancer."
Author(s): Matsumoto S, Hara T, Nagaoka M, Mike A, Mitsuyama K, Sako T, Yamamoto M, Kado S, Takada T
Number of Citations: 1
(See PubMed article )
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