| Details | |
| Host / Isotype: | Mouse / IgG1 |
| Class: | Monoclonal |
| Type: | Antibody |
| Clone: | E10022 |
| Species Reactivity: | Human (Hu) |
| Immunogen: | Recombinant human IL-4 purified from Pichia pastoris |
| Ordering Information | ||||
| Pierce IL-4 Antibody (E10022) |
| Storage: | 4° C |
| Form: | 500 ug of purified antibody from mouse ascites fluid by Protein G affinity chromatography and Lyophilized in PBS with 5% trehalose without preservative or carrier protein |
| Applications | Dilution * |
| ELISA (ELISA) | Assay Dependent |
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* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
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| Product Specific Information |
| M2IL4I targets IL-4 in ELISA applications and shows reactivity with Human samples. The M2IL4I immunogen is recombinant human IL-4 purified from Pichia pastoris. M2IL4I detects IL-4 which has a predicted molecular weight of approximately 15 kDa. |
| General Information |
| The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. |