IL-4 Antibody (E10021)

IL-4 Monoclonal Antibody for ELISA, Western blot

>> See 12 other antibodies for IL4 (B cell growth factor 1; BSF-1; B_cell stimulatory factor 1; binetrakin; interleukin-4; lymphocyte stimulatory factor 1; pitrakinra)
Synonyms:
B cell growth factor 1; BSF-1; B_cell stimulatory factor 1; binetrakin; interleukin-4; lymphocyte stimulatory factor 1; pitrakinra
Entrez Gene ID:
(Hu) 3565
UniProt ID:
(Hu) P05112
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: E10021
Species Reactivity: Human (Hu)
Immunogen: Recombinant human IL-4 purified from Pichia pastoris
Ordering Information
Pierce IL-4 Antibody (E10021)
Product #
MIL4I
Size
500 ug
Price
$299.00
Purchase
Add IL-4 Antibody (E10021) to your cart.
Storage: 4° C
Form: 500 ug of purified antibody from mouse ascites fluid by Protein G affinity chromatography and Lyophilized in PBS with 5% trehalose without preservative or carrier protein


Applications Dilution *
ELISA (ELISA) Assay Dependent
Western Blot (WB) Assay Dependent
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
MIL4I targets IL-4 in ELISA, and WB applications and shows reactivity with Human samples.

The MIL4I immunogen is recombinant human IL-4 purified from Pichia pastoris.

MIL4I detects IL-4 which has a predicted molecular weight of approximately 15 kDa.
General Information
The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
References:
Abrams, J.S., et al. (1992). Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol. Rev.127, 5-24.
Blotta, M.H., et al. (1996). Cross-linking of the CD40 ligand on human CD4+ T lymphocytes generates a costimulatory signal that up-regulates IL-4 synthesis. J. Immunol.156, 3133-3140.
Blotta, M.H., et al. (1997). Corticosteroids inhibit IL-12 production in human monocytes and enhance their capacity to induce IL-4 synthesis in CD4+ lymphocytes. J. Immunol.158, 5589-5595.
de Waal Malefyt, R., et al. (1993). Effects of IL-13 on phenotype, cytokine production, and cytotoxic function of human monocytes. J. Immunol.151, 6370-6381.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific