|Host / Isotype:||Rabbit / IgG|
|Species Reactivity:||Bovine (Bv)|
|Immunogen:||Recombinant bovine IL-4|
|Pierce IL-4 Antibody|
|Form:||1 mg of purified antibody from mouse ascites fluid by Protein G affinity chromatography and Lyophilized in PBS, without preservative or carrier protein|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
|Product Specific Information|
|PBOIL4I targets IL-4 in ELISA, and WB applications and shows reactivity with Bovine samples.
The PBOIL4I immunogen is recombinant bovine IL-4.
PBOIL4I detects IL-4 which has a predicted molecular weight of approximately 13 kDa.
|The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.|
|Species / Dilution||Summary|
Bv / Not Cited
PBOIL4I was used in ELISA to evaluate the diagnostic markers of Mycobacterium bovis infection in cattle
Clin Vaccine Immunol. 2010 Dec;17(12):1946-51.
"Simultaneous measurement of antigen-stimulated interleukin-1 beta and gamma interferon production enhances test sensitivity for the detection of Mycobacterium bovis infection in cattle."
Author(s): Jones GJ, Pirson C, Hewinson RG, Vordermeier HM
Number of Citations: 4
(See PubMed article )