| Details | |
| Host / Isotype: | Rabbit / IgG |
| Class: | Polyclonal |
| Type: | Antibody |
| Label: | Biotin |
| Species Reactivity: | Bovine (Bv) |
| Immunogen: | Recombinant Bovine IL-4 |
| Ordering Information | ||||
| Pierce IL-4 Antibody, Biotin conjugate |
| Storage: | 4° C |
| Form: | 250 ug of purified antibody from mouse ascites fluid by Protein G affinity chromatography and Lyophilized in PBS, without preservative or carrier protein |
| Applications | Dilution * |
| ELISA (ELISA) | Assay Dependent |
| Western Blot (WB) | Assay Dependent |
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* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
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| Product Specific Information |
| PBOIL4BI targets IL-4 in ELISA, and WB applications and shows reactivity with Bovine samples. The PBOIL4BI immunogen is recombinant Bovine IL-4. PBOIL4BI detects IL-4 which has a predicted molecular weight of approximately 13 kDa. |
| General Information |
| The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. |