IL-2R alpha / CD25 Antibody (IL2R.1)

IL-2R alpha / CD25 Monoclonal Antibody for IF, ICC, IHC (P), FACS

>> See 52 other antibodies for IL2RA (IL2 RA antibody, Il2ra antibody, Interleukin 2 receptor alpha chain antibody, TAC antigen antibody, IL-2R alpha chain)
Synonyms:
IL2 RA antibody, Il2ra antibody, Interleukin 2 receptor alpha chain antibody, TAC antigen antibody, IL-2R alpha chain
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgG1, kappa
Class: Monoclonal
Type: Antibody
Clone: IL2R.1
Tested Species Reactivity: Human (Hu), Mouse (Ms)
Published Species Reactivity: Human (Hu), Mouse (Ms)
Immunogen: Recombinant human interleukin-2 receptor
Ordering Information
Pierce IL-2R alpha / CD25 Antibody (IL2R.1)
Product #
MA5-12680
Size
500 ul
Price
$299.00
Purchase
Add IL-2R alpha / CD25 Antibody (IL2R.1) to your cart.
Tested Applications Dilution *
Immunofluorescence (IF) 1:10-1:100
Immunocytochemistry (ICC) 1:10-1:100
Immunohistochemistry (Paraffin) (IHC (P)) 1:100-1:500
Flow Cytometry (FACS) Assay Dependent
Published Applications Dilution
Western Blot (WB) See publications below
Immunohistochemistry (IHC) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 0.2mg/ml
Purification: Protein G
Storage Buffer: PBS, pH 7.4, with 0.2% BSA
Preservative: 0.09% sodium azide
Storage Conditions: 4° C
Product Specific Information
MA5-12680 targets CD25 in FACS, IHC (P) and IF applications and shows reactivity with Human and Mouse samples.

The MA5-12680 immunogen is recombinant human interleukin-2 receptor.
General Information
IL-2R (T-cell growth factor receptor) consists of a chain of 55kDa (also known as Tac antigen or p55 or alpha chain) and a p75 or beta chain. IL-2Ralpha is over-expressed on the surface of activated T-cells, HTLV-1 or -2 positive T-cell lines, and moderately expressed on activated B-cells, NK-cells, and macrophages.
Product Images
  • IL-2R alpha / CD25 Antibody (MA5-12680) in IF
    MA5-12680_IF_BAF-3_20131018121724.jpg

    Immunofluorescence with anti-IL-2R alpha / CD25 Monoclonal Antibody [IL2R.1] (MA5-12680) (MA5-12680_IF_BAF-3_20131018121724.jpg)

    Immunofluorescence with anti-IL-2R alpha / CD25 Monoclonal Antibody [IL2R.1] (MA5-12680)

    Immunofluorescent analysis of CD25 (green) showing staining in the cytoplasm of BAF-3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD25 monoclonal antibody (Product # MA5-12680) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
  • IL-2R alpha / CD25 Antibody (MA5-12680) in IHC (P)
    MA5-12680_IHC_human-tonsil-tissue.jpg

    Immunohistochemistry (Paraffin) with anti-IL-2R alpha / CD25 Monoclonal Antibody [IL2R.1] (MA5-12680) (MA5-12680_IHC_human-tonsil-tissue.jpg)

    Immunohistochemistry (Paraffin) with anti-IL-2R alpha / CD25 Monoclonal Antibody [IL2R.1] (MA5-12680)

    Immunohistochemistry analysis of CD25 showing positive staining in the membrane of paraffin-treated Human tonsil tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a CD25 monoclonal antibody (Product # MA5-12680) diluted by 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
  • IL-2R alpha / CD25 Antibody (MA5-12680) in IHC (P)
    MA5-12680_IHC_human-spleen-tissue.jpg

    Immunohistochemistry (Paraffin) with anti-IL-2R alpha / CD25 Monoclonal Antibody [IL2R.1] (MA5-12680) (MA5-12680_IHC_human-spleen-tissue.jpg)

    Immunohistochemistry (Paraffin) with anti-IL-2R alpha / CD25 Monoclonal Antibody [IL2R.1] (MA5-12680)

    Immunohistochemistry analysis of CD25 showing positive staining in the membrane of paraffin-treated Human spleen tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a CD25 monoclonal antibody (Product # MA5-12680) diluted by 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
  • IL-2R alpha / CD25 Antibody (MA5-12680) in FACS
    MA5-12680_FACS_Jurkat.jpg

    Flow Cytometry with anti-IL-2R alpha / CD25 Monoclonal Antibody [IL2R.1] (MA5-12680) (MA5-12680_FACS_Jurkat.jpg)

    Flow Cytometry with anti-IL-2R alpha / CD25 Monoclonal Antibody [IL2R.1] (MA5-12680)

    Flow cytometry analysis of CD25 in Jurkat cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD25 monoclonal antibody (Product # MA5-12680) at a dilution of 1 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.
  • IL-2R alpha / CD25 Antibody (MA5-12680) in FACS
    MA5-12680_FACS_BAF-3.jpg

    Flow Cytometry with anti-IL-2R alpha / CD25 Monoclonal Antibody [IL2R.1] (MA5-12680) (MA5-12680_FACS_BAF-3.jpg)

    Flow Cytometry with anti-IL-2R alpha / CD25 Monoclonal Antibody [IL2R.1] (MA5-12680)

    Flow cytometry analysis of CD25 in BAF-3 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD25 monoclonal antibody (Product # MA5-12680) at a dilution of 0.5 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.
Publications:
Western Blot
  Species / Dilution Summary
  Hu / Not Cited
MA5-12680 was used in western blot to investigate the involvement of beta3 integrin and c-Src in focal adhesion complex formation in adult cardiomyocytes

J Mol Cell Cardiol. 2003 Jun;35(6):671-83.
"Focal complex formation in adult cardiomyocytes is accompanied by the activation of beta3 integrin and c-Src."
Author(s): Willey CD, Balasubramanian S, Rodríguez Rosas MC, Ross RS, Kuppuswamy D
Number of Citations: 6
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  Hu / 1:60
MA5-12680 was used in immunohistochemistry to investigate the influence of FOXP3 T regulatory cells in the pathogenesis of oral lichen planus

Oral Dis. 2010 Jan;16(1):76-82.
"FOXP3 T regulatory cells in lesions of oral lichen planus correlated with disease activity."
Author(s): Tao XA, Xia J, Chen XB, Wang H, Dai YH, Rhodus NL, Cheng B
Number of Citations: 0
(See PubMed article )
  Hu / 1:200
MA5-12680 was used in immunohistochemistry to study CD25 as a new marker of bile canaliculus

Int J Surg Pathol. 2012 Oct;20(5):455-61.
"CD25 is a novel marker of hepatic bile canaliculus."
Author(s): Lu ZH, -Chen W, Ju CX, -Den J, Kuai SG, Pei H, Huang LH, Gu XB, Ying YX, Hu XQ, Zhu TF, Xing YP, Fan QH
Number of Citations: 0
(See PubMed article )
  Ms / Not Cited
MA5-12680 was used in immunohistochemistry to study regulatory T cell activity and liver pathology in a mouse model of graft-versus-host response

Hepatol Res. 2009 Jun;39(6):585-94.
"Regulatory T cells and liver pathology in a murine graft versus host response model."
Author(s): Miyazaki T, Doy M, Unno R, Honda A, Ikegami T, Itoh S, Bouscarel B, Matsuzaki Y
Number of Citations: 0
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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