IL-13 Antibody, Biotin conjugate (5A2)

IL-13 Monoclonal Antibody for Western blot, ELISA

>> See 10 other antibodies for IL13 (interleukin-13)
Entrez Gene ID:
UniProt ID:
Host / Isotype: Rat / IgG
Class: Monoclonal
Type: Antibody
Clone: 5A2
Label: Biotin
Tested Species Reactivity: Human (Hu)
Published Species Reactivity: Human (Hu)
Immunogen: Recombinant human IL-14
Ordering Information
Pierce IL-13 Antibody, Biotin conjugate (5A2)
Product #
250 ug
Tested Applications Dilution *
Western Blot (WB) Assay Dependent
ELISA (ELISA) Assay Dependent
Published Applications Dilution
ELISA (ELISA) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 0.5mg/ml
Purification: Protein A
Storage Buffer: PBS with 4% BSA
Preservative: no preservative
Storage Conditions: -20°C
Product Specific Information
M130B targets IL-13 in ELISA, and WB applications and shows reactivity with Human samples.

The M130B immunogen is recombinant human IL-14. This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.

M130B detects IL-13 which has a predicted molecular weight of approximately 13 kDa.

The M130B IL13 antibody (biotinylated conjugate of clone 5A2) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody P130E. Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection.

Biotinylated antibody M130B (clone 5A2) and antibody P130E have successfully been used in combination with recombinant IL13 protein SIL13 in ELISA applications.
General Information
This gene encodes an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4.
  Species / Dilution Summary
  Hu / Not Cited
M130B was used in ELISA to study the effect of the attachment (G) glycoprotein from respiratory synctial virus on wheezing

J Infect Dis. 2001 Dec 15;184(12):1589-93.
"Adaptive immune responses of patients with asthma to the attachment (G) glycoprotein of respiratory synctial virus."
Author(s): Hancock GE, Scheuer CA, Sierzega R, Pryharski KS, McBride JT, Watelet LF, Tebbey PW, Smith JD
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
M130B was used in ELISA to investigate the effect of Bordetella pertussis infection on type 1 T cell responses

J Immunol. 2003 Feb 1;170(3):1504-9.
"Bordetella pertussis infection in 2-month-old infants promotes type 1 T cell responses."
Author(s): Mascart F, Verscheure V, Malfroot A, Hainaut M, Piérard D, Temerman S, Peltier A, Debrie AS, Levy J, Del Giudice G, Locht C
Number of Citations: 15
(See PubMed article )
  Hu / Not Cited
M130B was used in ELISA to characterize the prenatal immune responses against Plasmodium falciparum erythrocyte invasion ligands

Infect Immun. 2005 Jun;73(6):3462-70.
"Distinct Th1- and Th2-Type prenatal cytokine responses to Plasmodium falciparum erythrocyte invasion ligands."
Author(s): Malhotra I, Mungai P, Muchiri E, Ouma J, Sharma S, Kazura JW, King CL
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
M130B was used in ELISA to study the interleukin 13 production in infants with different pertussis-tetanus-diphtheria-poliomyelitis vaccines

Vaccine. 2007 Jan 4;25(2):391-8.
"Modulation of the infant immune responses by the first pertussis vaccine administrations."
Author(s): Mascart F, Hainaut M, Peltier A, Verscheure V, Levy J, Locht C
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
M130B was used in ELISA to study the mucosal production of interleukin 13 in Helicobacter pylori-related gastric diseases

Dig Liver Dis. 2008 Apr;40(4):240-7.
"Interleukin-13 mucosal production in Helicobacter pylori-related gastric diseases."
Author(s): Marotti B, Rocco A, De Colibus P, Compare D, de Nucci G, Staibano S, Tatangelo F, Romano M, Nardone G
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
M130B was used in ELISA to study the effect of the prenatal malaria exposure on immune tolerance

PLoS Med. 2009 Jul;6(7):e1000116.
"Can prenatal malaria exposure produce an immune tolerant phenotype? A prospective birth cohort study in Kenya."
Author(s): Malhotra I, Dent A, Mungai P, Wamachi A, Ouma JH, Narum DL, Muchiri E, Tisch DJ, King CL
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
M130B was used in ELISA to study the role of HLA-DQ molecules in Th-cell responses

Lab Invest. 2010 Dec;90(12):1747-56.
"Fibroblasts stimulated via HLA-II molecules produce prostaglandin E? and regulate cytokine production from helper T cells."
Author(s): Kato-Kogoe N, Ohyama H, Nishimura F, Meguro M, Yoshizawa S, Okada Y, Nakasho K, Yamanegi K, Yamada N, Hata M, Higashi T, Terada N, Matsushita S
Number of Citations: 1
(See PubMed article )
  Hu / 0
M130B was used in ELISA to study cellular immune responses in HIV-1-infected children

Clin Exp Immunol. 2011 Jul;165(1):77-84.
"Cellular immune responses in human immunodeficiency virus (HIV)-1-infected children: is immune restoration by highly active anti-retroviral therapy comparable to non-progression?"
Author(s): Hainaut M, Verscheure V, Ducarme M, Schandené L, Levy J, Mascart F
Number of Citations: 0
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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