IL-13 Antibody

IL-13 Polyclonal Antibody for Western blot, ELISA

>> See 7 other antibodies for IL13 (interleukin-13)
Synonyms:
interleukin-13
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit / IgG
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu)
Published Species Reactivity: Human (Hu)
Immunogen: Recombinant human IL-13
Ordering Information
Pierce IL-13 Antibody
Product #
P130E
Size
1 mg
Price
$316.00
Purchase
Add IL-13 Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) Assay Dependent
ELISA (ELISA) Assay Dependent
Published Applications Dilution
ELISA (ELISA) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 0.93mg/ml
Purification: Protein A
Storage Buffer: PBS
Preservative: no preservative
Storage Conditions: -20°C
Product Specific Information
P130E targets IL-13 in ELISA, and WB applications and shows reactivity with Human samples.

The P130E immunogen is recombinant human IL-13.

P130E detects IL-13 which has a predicted molecular weight of approximately 13 kDa.

The P130E IL13 polyclonal antibody has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M130B (biotinylated conjugate of clone 5A2) Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection.

Antibody P130E and biotinylated antibody M130B (clone 5A2) have successfully been used in combination with recombinant IL13 protein SIL13 in ELISA applications.
General Information
This gene encodes an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4.
Publications:
ELISA
  Species / Dilution Summary
  Hu / 1:2,000
P130E was used in ELISA to investigate the role of plasmodium vivax duffy binding protein

J Immunol. 2002 Sep 15;169(6):3200-7.
"Age-dependent cellular immune responses to Plasmodium vivax Duffy binding protein in humans."
Author(s): Xainli J, Baisor M, Kastens W, Bockarie M, Adams JH, King CL
Number of Citations: 7
(See PubMed article )
  Hu / Not Cited
P130E was used in ELISA to characterize the prenatal immune responses against Plasmodium falciparum erythrocyte invasion ligands

Infect Immun. 2005 Jun;73(6):3462-70.
"Distinct Th1- and Th2-Type prenatal cytokine responses to Plasmodium falciparum erythrocyte invasion ligands."
Author(s): Malhotra I, Mungai P, Muchiri E, Ouma J, Sharma S, Kazura JW, King CL
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
P130E was used in ELISA to study the interleukin 13 production in infants with different pertussis-tetanus-diphtheria-poliomyelitis vaccines

Vaccine. 2007 Jan 4;25(2):391-8.
"Modulation of the infant immune responses by the first pertussis vaccine administrations."
Author(s): Mascart F, Hainaut M, Peltier A, Verscheure V, Levy J, Locht C
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
P130E was used in ELISA to study the effect of the prenatal malaria exposure on immune tolerance

PLoS Med. 2009 Jul;6(7):e1000116.
"Can prenatal malaria exposure produce an immune tolerant phenotype? A prospective birth cohort study in Kenya."
Author(s): Malhotra I, Dent A, Mungai P, Wamachi A, Ouma JH, Narum DL, Muchiri E, Tisch DJ, King CL
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
P130E was used in ELISA to study the role of HLA-DQ molecules in Th-cell responses

Lab Invest. 2010 Dec;90(12):1747-56.
"Fibroblasts stimulated via HLA-II molecules produce prostaglandin E? and regulate cytokine production from helper T cells."
Author(s): Kato-Kogoe N, Ohyama H, Nishimura F, Meguro M, Yoshizawa S, Okada Y, Nakasho K, Yamanegi K, Yamada N, Hata M, Higashi T, Terada N, Matsushita S
Number of Citations: 1
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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