IL-1 beta Antibody (ILB1-H67)

IL-1 beta Monoclonal Antibody for Western blot, ELISA

>> See 19 other antibodies for IL1B (IL-1 beta; catabolin; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta)
Synonyms:
IL-1 beta; catabolin; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgG2b
Class: Monoclonal
Type: Antibody
Clone: ILB1-H67
Tested Species Reactivity: Human (Hu)
Published Species Reactivity: Human (Hu)
Immunogen: Recombinant human IL-1 beta
Ordering Information
Pierce IL-1 beta Antibody (ILB1-H67)
Product #
M421B
Size
500 ug
Price
$316.00
Purchase
Add IL-1 beta Antibody (ILB1-H67) to your cart.
Tested Applications Dilution *
Western Blot (WB) Assay Dependent
ELISA (ELISA) Assay Dependent
Published Applications Dilution
Immunohistochemistry (IHC) See publications below
ELISA (ELISA) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 0.79mg/ml
Purification: Protein G
Storage Buffer: PBS
Preservative: no preservative
Storage Conditions: -20°C
Product Specific Information
M421B targets IL-1 beta in ELISA, and WB applications and shows reactivity with Human samples.

A precipitate may be present in the solution. Mix for at least five seconds prior to use.

M421B detects IL-1 beta which has a predicted molecular weight of approximately 31 kDa.

The M421B IL1b antibody (clone ILB1-H67) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M420BB (biotinylated conjugate of clone ILB1-H6). Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection.

Antibody M421B (clone ILB1-H67) and biotinylated antibody M420BB (clone ILB1-H6) have successfully been used in combination with recombinant IL1 beta protein SIL1B in ELISA applications.
General Information
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.
Product Images
  • IL-1 beta Antibody (M421B) in ELISA
    IL-1-beta-biotin_Antibody_M421B_ELISA_1_20130515075127.jpg

    ELISA with anti-IL-1 beta Monoclonal Antibody [ILB1-H67] (M421B) (IL-1-beta-biotin_Antibody_M421B_ELISA_1_20130515075127.jpg)

    ELISA with anti-IL-1 beta Monoclonal Antibody [ILB1-H67] (M421B)

    Sandwich ELISA analysis of human IL-1b was performed using a Human IL-1b Colorimetric ELISA Kit (Product # EH2IL1B) by loading 50 ul per well of Human IL-1b Recombinant Protein (Product # SIL1B) in quadruplicate at 400, 160, 64, 25.6, 10.24 and 0 pg/ml across a 5 ug/ml mouse anti-human IL-1b (Product # M421B) pre-coated plate and incubating for 3 hours at room temperature. The plate was washed, then incubated with 50 ul per well of a biotinylated-mouse anti-human IL-1b (Product # M420BB) in quadruplicate at 0.07 ug/ml for 3 hours at room temperature. The plate was washed and incubated with 100 ul per well of Ultra Streptavidin-HRP (Product # N504) in all test wells at 1:3000 for 30 minutes at room temperature. Detection was performed using 1-Step Ultra TMB Substrate (Product # 34028) for 30 minutes at room temperature in the dark. The plate was then stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550 nm.
Publications:
Immunohistochemistry
  Species / Dilution Summary
  Hu / Not Cited
M421B was used in immunohistochemistry to investigate the intrahepatic expression of interleukin 1 beta and tumor necrosis factor alpha in liver specimens with chronic hepatitis C

J Infect Dis. 1999 Nov;180(5):1704-8.
"Intrahepatic expression of interleukin-1beta and tumor necrosis factor-alpha in chronic hepatitis C."
Author(s): Dumoulin FL, Leifeld L, Honecker U, Sauerbruch T, Spengler U
Number of Citations: 1
(See PubMed article )
ELISA
  Species / Dilution Summary
  Hu / Not Cited
M421B was used in ELISA to assess the effect of complement receptor 3 on interleukin 12 production

J Exp Med. 1997 Jun 2;185(11):1987-95.
"Regulation of interleukin-12 by complement receptor 3 signaling."
Author(s): Marth T, Kelsall BL
Number of Citations: 42
(See PubMed article )
  Hu / Not Cited
M421B was used in ELISA to investigate the importance of PGE2 during dendritic cell development

J Immunol. 1997 Jul 1;159(1):28-35.
"IL-12-deficient dendritic cells, generated in the presence of prostaglandin E2, promote type 2 cytokine production in maturing human naive T helper cells."
Author(s): Kali?ski P, Hilkens CM, Snijders A, Snijdewint FG, Kapsenberg ML
Number of Citations: 51
(See PubMed article )
  Hu / Not Cited
M421B was used in ELISA to investigate the roles of prostaglandin E2 and interleukin 10 in CD1a+ dendritic cell maturation

J Immunol. 1998 Sep 15;161(6):2804-9.
"Prostaglandin E2 induces the final maturation of IL-12-deficient CD1a+CD83+ dendritic cells: the levels of IL-12 are determined during the final dendritic cell maturation and are resistant to further modulation."
Author(s): Kali?ski P, Schuitemaker JH, Hilkens CM, Kapsenberg ML
Number of Citations: 36
(See PubMed article )
  Hu / 1 ug/ml
M421B was used in ELISA to investigate the role of interleukin 1 beta during the cell death induced by HIV infection

Br J Pharmacol. 2001 Nov;134(6):1344-50.
"HIV-1 coat protein gp120 stimulates interleukin-1beta secretion from human neuroblastoma cells: evidence for a role in the mechanism of cell death."
Author(s): Corasaniti MT, Bilotta A, Strongoli MC, Navarra M, Bagetta G, Di Renzo G
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
M421B was used in ELISA to study the effect of etanercept treatment on proinflammatory cytokine-secreting peripheral blood mononuclear cells in rheumatoid arthritic patients

Rheumatology (Oxford). 2004 Aug;43(8):960-4.
"Long-term treatment with etanercept significantly reduces the number of proinflammatory cytokine-secreting peripheral blood mononuclear cells in patients with rheumatoid arthritis."
Author(s): Schotte H, Schlüter B, Willeke P, Mickholz E, Schorat MA, Domschke W, Gaubitz M
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
M421B was used in ELISA to investigate the effects of antipyretics on cytokine levels in uncomplicated falciparum malaria

Acta Trop. 2006 Aug;99(1):75-82.
"Relationship between antipyretic effects and cytokine levels in uncomplicated falciparum malaria during different treatment regimes."
Author(s): Hugosson E, Montgomery SM, Premji Z, Troye-Blomberg M, Björkman A
Number of Citations: 1
(See PubMed article )
  Hu / 1.5 ug/ml
M421B was used in ELISA to study the role of Th1 responses to beta amyloid in Down syndrome and Alzheimer disease

Neurobiol Aging. 2010 Oct;31(10):1732-42.
"Th1 responses to beta-amyloid in young humans convert to regulatory IL-10 responses in Down syndrome and Alzheimer's disease."
Author(s): Loewenbrueck KF, Tigno-Aranjuez JT, Boehm BO, Lehmann PV, Tary-Lehmann M
Number of Citations: 1
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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