IL-1 beta Antibody, Biotin conjugate (ILB1-H6)

IL-1 beta Monoclonal Antibody for ELISA

>> See 19 other antibodies for IL1B (IL-1 beta; catabolin; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta)
Synonyms:
IL-1 beta; catabolin; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgG1, kappa
Class: Monoclonal
Type: Antibody
Clone: ILB1-H6
Label: Biotin
Tested Species Reactivity: Human (Hu)
Published Species Reactivity: Human (Hu)
Immunogen: Recombinant human IL-1 beta
Ordering Information
Pierce IL-1 beta Antibody, Biotin conjugate (ILB1-H6)
Product #
M420BB
Size
250 µg
Price
$316.00
Purchase
Add IL-1 beta Antibody, Biotin conjugate (ILB1-H6) to your cart.
Tested Applications Dilution *
ELISA (ELISA) Assay Dependent
Published Applications Dilution
ELISA (ELISA) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 0.5mg/ml
Purification: Protein G
Storage Buffer: PBS with 4% BSA
Preservative: proprietary Preservative
Storage Conditions: -20°C
Product Specific Information
M420BB targets IL-1 beta in ELISA applications and shows reactivity with Human samples.

The M420BB immunogen is recombinant human IL-1 beta.

M420BB detects IL-1 beta which has a predicted molecular weight of approximately 31 kDa.

The M420BB IL1b antibody (biotinylated conjugate of clone ILB1-H6) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody M421B (clone ILB1-H67). Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection.

Biotinylated antibody M420BB (clone ILB1-H6) and antibody M421B (clone ILB1-H67) have successfully been used in combination with recombinant IL1 beta protein SIL1B in ELISA applications.
General Information
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.
Product Images
  • IL-1 beta Antibody (M420BB) in ELISA
    M420BB image for website.jpg

    ELISA with anti-IL-1 beta Monoclonal Antibody [ILB1-H6], Biotin conjugate (M420BB) (M420BB image for website.jpg)

    ELISA with anti-IL-1 beta Monoclonal Antibody [ILB1-H6], Biotin conjugate (M420BB)

    Sandwich ELISA analysis of Mouse anti-Human IL-1beta biotinylated monoclonal antibody (Product #M420BB) was performed using the Thermo Scientific ELISA kit EH2IL1B by loading 50µl per well of Human IL-1beta recombinant protein (Product #SIL1B) in quadruplicate at 400, 160, 64, 25.6, 10.24 and 0 pg/ml across a 5µg/ml Mouse anti-Human IL-1beta (Product #M421B) pre-coated plate and incubated for 3 hours at room temperature. The plate was washed then incubated with 50µl per well of Mouse anti-Human IL-1beta biotinylated antibody (Product #M420BB) in quadruplicate at 0.07µg/ml for 3 hours at room temperature. The plate was washed and incubated with 100µl per well of Ultra Streptavidin-HRP (Product #N504) in all test wells at 1:3000 for 30 minutes at room temperature. The plate was washed then incubated with 100µl per well of Ultra TMB substrate (Product #34028) for 30 minutes at room temperature in the dark. The plate was then stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550nm.
Publications:
ELISA
  Species / Dilution Summary
  Hu / Not Cited
M420BB was used in ELISA to investigate the role of mast cells/basophils in dengue pathogenesis

J Virol. 2000 Aug;74(15):7146-50.
"Release of vasoactive cytokines by antibody-enhanced dengue virus infection of a human mast cell/basophil line."
Author(s): King CA, Marshall JS, Alshurafa H, Anderson R
Number of Citations: 7
(See PubMed article )
  Hu / 0.05 ug/ml
M420BB was used in ELISA to investigate the role of interleukin 1 beta during the cell death induced by HIV infection

Br J Pharmacol. 2001 Nov;134(6):1344-50.
"HIV-1 coat protein gp120 stimulates interleukin-1beta secretion from human neuroblastoma cells: evidence for a role in the mechanism of cell death."
Author(s): Corasaniti MT, Bilotta A, Strongoli MC, Navarra M, Bagetta G, Di Renzo G
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
M420BB was used in ELISA to investigate the role of calcium ion in P2X7 nucleotide receptor-mediated interleukin 1 beta secretion

Am J Physiol Cell Physiol. 2003 Aug;285(2):C286-99.
"Essential role for Ca2+ in regulation of IL-1beta secretion by P2X7 nucleotide receptor in monocytes, macrophages, and HEK-293 cells."
Author(s): Gudipaty L, Munetz J, Verhoef PA, Dubyak GR
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
M420BB was used in ELISA to study the effect of etanercept treatment on proinflammatory cytokine-secreting peripheral blood mononuclear cells in rheumatoid arthritic patients

Rheumatology (Oxford). 2004 Aug;43(8):960-4.
"Long-term treatment with etanercept significantly reduces the number of proinflammatory cytokine-secreting peripheral blood mononuclear cells in patients with rheumatoid arthritis."
Author(s): Schotte H, Schlüter B, Willeke P, Mickholz E, Schorat MA, Domschke W, Gaubitz M
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
M420BB was used in ELISA to investigate the reliability of the whole blood assay for the detection of immune response induced by bioaerosols

J Vet Med B Infect Dis Vet Public Health. 2006 May;53(4):176-80.
"Determination of the inflammatory potential of bioaerosols from a duck-fattening unit by using a limulus amebocyte lysate assay and human whole blood cytokine response."
Author(s): Zucker BA, Scharf P, Kersten C
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
M420BB was used in ELISA to investigate the effects of antipyretics on cytokine levels in uncomplicated falciparum malaria

Acta Trop. 2006 Aug;99(1):75-82.
"Relationship between antipyretic effects and cytokine levels in uncomplicated falciparum malaria during different treatment regimes."
Author(s): Hugosson E, Montgomery SM, Premji Z, Troye-Blomberg M, Björkman A
Number of Citations: 1
(See PubMed article )
  Hu / 0
M420BB was used in ELISA to develop a protein microarray platform for the multiplex analysis of biomarkers in human sera

J Immunol Methods. 2006 Oct 20;316(1-2):1-7.
"Protein microarray platform for the multiplex analysis of biomarkers in human sera."
Author(s): Urbanowska T, Mangialaio S, Zickler C, Cheevapruk S, Hasler P, Regenass S, Legay F
Number of Citations: 7
(See PubMed article )
  Hu / 2 ug/ml
M420BB was used in ELISA to study the role of Th1 responses to beta amyloid in Down syndrome and Alzheimer disease

Neurobiol Aging. 2010 Oct;31(10):1732-42.
"Th1 responses to beta-amyloid in young humans convert to regulatory IL-10 responses in Down syndrome and Alzheimer's disease."
Author(s): Loewenbrueck KF, Tigno-Aranjuez JT, Boehm BO, Lehmann PV, Tary-Lehmann M
Number of Citations: 1
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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