IKK gamma Antibody (PA5-28606) in WB
IKK-gamma_PA5-28606_Antibody_WesternBlot.jpgWestern Blot analysis of IKK gamma using anti-IKK gamma Polyclonal Antibody (PA5-28606), in Jurkat Cell Lysate (30 ug of cell lysate) and at a dilution of 1:3000.
|Host / Isotype:||Rabbit / IgG|
|Tested Species Reactivity:||Human (Hu), Mouse (Ms), Rat (Rt)|
|Immunogen:||Recombinant fragment corresponding to a region within amino acids 1 and 226 of Human IKK gamma|
|Pierce IKK gamma Antibody|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
|Purification:||Antigen affinity chromatography|
|Storage Buffer:||0.1M tris, pH 7, with 10% glycerol, 0.1M glycine|
|Storage Conditions:||-20° C, Avoid Freeze/Thaw Cycles|
|Product Specific Information|
|PA5-28606 targets IKK gamma in IF, IHC (P), and WB applications and shows reactivity with Human, Mouse, and Rat samples.
The PA5-28606 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 226 of Human IKK gamma.
|This gene encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome.|