| Details | |
| Host / Isotype: | Mouse / IgG1 |
| Class: | Monoclonal |
| Type: | Antibody |
| Clone: | CC302 |
| Species Reactivity: | Goat (Gt) Mustelid (Md) Human (Hu) Canine (Ca) Bovine (Bv) Ovine (Ov) Porcine (Po) Equine (Eq) |
| Immunogen: | Recombinant Bovine IFN gamma |
| Ordering Information | ||||
| Pierce IFN gamma Antibody (CC302) |
| Storage: | -80°C |
| Form: | 500 ug of purified antibody from tissue culture supernatant by Protein G affinity chromatography; in PBS preservative free |
| Applications | Dilution * |
| Flow Cytometry (FACS) | Assay Dependent |
| ELISA (ELISA) | Assay Dependent |
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* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
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| Product Specific Information |
| MBOIFNG targets IFN gamma in ELISA, and FACS applications and shows reactivity with Bovine, Canine, Equine, Goat, Human, Mustelid, Ovine, and Porcine samples. The MBOIFNG immunogen is recombinant Bovine IFN gamma. MBOIFNG detects IFN gamma which has a predicted molecular weight of approximately 17 kDa. |
| General Information |
| IFN gamma, also called Type II interferon, macrophage activation factor, and immune interferon is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE it appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific and includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFNg is involved in cytokine regulation and also acts synergistically with other cytokines. |