* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
PBOIFNGBI targets IFN gamma in ELISA applications and shows reactivity with Bovine samples.
The PBOIFNGBI immunogen is recombinant Bovine IFN gamma.
PBOIFNGBI detects IFN gamma which has a predicted molecular weight of approximately 17 kDa.
The PBOIFNGBI polyclonal biotinylated bovine IFNg antibody has successfully been paired as the detection antibody in a sandwich ELISA with polyclonal capture antibody PBOIFNGI. Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection.
IFN gamma, also called Type II interferon, macrophage activation factor, and immune interferon is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE it appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific and includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFNg is involved in cytokine regulation and also acts synergistically with other cytokines.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)