| Details | |
| Host / Isotype: | Rabbit / IgG |
| Class: | Polyclonal |
| Type: | Antibody |
| Label: | Biotin |
| Species Reactivity: | Bovine (Bv) |
| Immunogen: | Recombinant Bovine IFN gamma |
| Ordering Information | ||||
| Pierce IFN gamma Antibody, Biotin conjugate |
| Storage: | 4° C |
| Form: | 1 mg of antibodies lyophilized in PBS, preservative and carrier free |
| Applications | Dilution * |
| ELISA (ELISA) | Assay Dependent |
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* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
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| Product Specific Information |
| PBOIFNGBI targets IFN gamma in ELISA applications and shows reactivity with Bovine samples. The PBOIFNGBI immunogen is recombinant Bovine IFN gamma. PBOIFNGBI detects IFN gamma which has a predicted molecular weight of approximately 17 kDa. The PBOIFNGBI polyclonal biotinylated bovine IFNg antibody has successfully been paired as the detection antibody in a sandwich ELISA with polyclonal capture antibody PBOIFNGI. Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection. |
| General Information |
| IFN gamma, also called Type II interferon, macrophage activation factor, and immune interferon is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE it appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific and includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFNg is involved in cytokine regulation and also acts synergistically with other cytokines. |