IDH1 Antibody

IDH1 Polyclonal Antibody for Western blot, IF, ICC, IHC (P), IP

>> See 6 other antibodies for IDH1 (IDCD, IDH, IDP, IDPC, PICD, NADP(+)-specific ICDH, NADP-dependent isocitrate dehydrogenase, cytosolic, NADP-dependent isocitrate dehydrogenase, peroxisomal, isocitrate dehydrogenase [NADP] cytoplasmic, oxalosuccinate decarboxylase)
Synonyms:
IDCD, IDH, IDP, IDPC, PICD, NADP(+)-specific ICDH, NADP-dependent isocitrate dehydrogenase, cytosolic, NADP-dependent isocitrate dehydrogenase, peroxisomal, isocitrate dehydrogenase [NADP] cytoplasmic, oxalosuccinate decarboxylase
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit / IgG
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu), Mouse (Ms)
Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 190 of Human IDH1
Ordering Information
Pierce IDH1 Antibody
Product #
PA5-30067
Size
100 µL
Price
$324.00
Purchase
Tested Applications Dilution *
Western Blot (WB) 1:5000-1:20,000
Immunofluorescence (IF) 1:100-1:1000
Immunocytochemistry (ICC) 1:100-1:1000
Immunohistochemistry (Paraffin) (IHC (P)) 1:100-1:1000
Immunoprecipitation (IP) 1:500-1:1000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 0.83mg/ml
Purification: Antigen affinity chromatography
Storage Buffer: 0.1M tris, pH 7, with 20% glycerol, 0.1M glycine
Preservative: 0.01% thimerosal
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA5-30067 targets IDH1 in IF, IP, IHC (P), and WB applications and shows reactivity with Human and Mouse samples.

The PA5-30067 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 190 of Human IDH1.
General Information
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.
Product Images
  • IDH1 Antibody (PA5-30067) in WB
    PA5-30067_WB_1.jpg

    Western Blot with anti-IDH1 Polyclonal Antibody (PA5-30067) (PA5-30067_WB_1.jpg)

    Western Blot with anti-IDH1 Polyclonal Antibody (PA5-30067)

    Western blot analysis of IDH1 using A) 30 µg A431 whole cell lysate and B) 30 µg HepG2 whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an IDH1 polyclonal antibody (Product # PA5-30067) at a dilution of 1:5000.
  • IDH1 Antibody (PA5-30067) in WB
    PA5-30067_WB_2.jpg

    Western Blot with anti-IDH1 Polyclonal Antibody (PA5-30067) (PA5-30067_WB_2.jpg)

    Western Blot with anti-IDH1 Polyclonal Antibody (PA5-30067)

    Western blot analysis of IDH1 using A) 30 µg Neuro2A whole cell lysate (B) 30 µg GL261 whole cell lysate (C) 30 µg C8D30 whole cell lysate (D) 30 µg NIH-3T3 whole cell lysate (E) 30 µg Raw264.7 whole cell lysate and F) 30 µg C2C12 whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an IDH1 polyclonal antibody (Product # PA5-30067) at a dilution of 1:10000.
  • IDH1 Antibody (PA5-30067) in IF
    PA5-30067_IF.jpg

    Immunofluorescence with anti-IDH1 Polyclonal Antibody (PA5-30067) (PA5-30067_IF.jpg)

    Immunofluorescence with anti-IDH1 Polyclonal Antibody (PA5-30067)

    Immunofluorescent analysis of IDH1 in methanol-fixed A431 cells using an IDH1 polyclonal antibody (Product # PA5-30067) at a 1:500 dilution.
  • IDH1 Antibody (PA5-30067) in IHC (P)
    PA5-30067_IHC.jpg

    Immunohistochemistry (Paraffin) with anti-IDH1 Polyclonal Antibody (PA5-30067) (PA5-30067_IHC.jpg)

    Immunohistochemistry (Paraffin) with anti-IDH1 Polyclonal Antibody (PA5-30067)

    Immunohistochemical analysis of IDH1 in paraffin-embedded breast carcinoma using an IDH1 polyclonal antibody (Product # PA5-30067) at a dilution of 1:500.
  • IDH1 Antibody (PA5-30067) in IP
    PA5-30067_IP.jpg

    Immunoprecipitation with anti-IDH1 Polyclonal Antibody (PA5-30067) (PA5-30067_IP.jpg)

    Immunoprecipitation with anti-IDH1 Polyclonal Antibody (PA5-30067)

    PA5-30067 immunoprecipitates IDH1 protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 ?g HeLa whole cell lysate/extract B. Control with 2.5 ?g of preimmune rabbit IgG C. Immunoprecipitation of IDH1 protein by 2.5 ?g of IDH1 antibody (Product # PA5-30067) on 12% SDS-PAGE.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific