IDH1 Antibody

IDH1 Polyclonal Antibody for Western blot, IF, ICC, IHC (P), IP

>> See 6 other antibodies for IDH1 (IDCD, IDH, IDP, IDPC, PICD, NADP(+)-specific ICDH, NADP-dependent isocitrate dehydrogenase, cytosolic, NADP-dependent isocitrate dehydrogenase, peroxisomal, isocitrate dehydrogenase [NADP] cytoplasmic, oxalosuccinate decarboxylase)
Synonyms:
IDCD, IDH, IDP, IDPC, PICD, NADP(+)-specific ICDH, NADP-dependent isocitrate dehydrogenase, cytosolic, NADP-dependent isocitrate dehydrogenase, peroxisomal, isocitrate dehydrogenase [NADP] cytoplasmic, oxalosuccinate decarboxylase
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit / IgG
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu), Mouse (Ms)
Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 190 of Human IDH1
Ordering Information
Pierce IDH1 Antibody
Product #
PA5-30067
Size
100 ul
Price
$316.00
Purchase
Add IDH1 Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:5000-1:20,000
Immunofluorescence (IF) 1:100-1:1000
Immunocytochemistry (ICC) 1:100-1:1000
Immunohistochemistry (Paraffin) (IHC (P)) 1:100-1:1000
Immunoprecipitation (IP) 1:500-1:1000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 0.83mg/ml
Purification: Antigen affinity chromatography
Storage Buffer: 0.1M tris, pH 7, with 20% glycerol, 0.1M glycine
Preservative: 0.01% thimerosal
Storage Conditions: -20°C
Product Specific Information
PA5-30067 targets IDH1 in IF, IP, IHC (P), and WB applications and shows reactivity with Human and Mouse samples.

The PA5-30067 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 190 of Human IDH1.
General Information
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.
Product Images
  • IDH1 Antibody (PA5-30067) in WB
    IDH1_PA5-30067_Antibody_WesternBlot.jpg

    Western Blot with anti-IDH1 Polyclonal Antibody (PA5-30067) (IDH1_PA5-30067_Antibody_WesternBlot.jpg)

    Western Blot with anti-IDH1 Polyclonal Antibody (PA5-30067)

    Western Blot analysis of IDH1 using anti-IDH1 Polyclonal Antibody (PA5-30067), in Raji Cell Lysate (30 ug of cell lysate) and at a dilution of 1:1000.
  • IDH1 Antibody (PA5-30067) in IF
    IDH1_PA5-30067_Antibody_Immunofluorescence.jpg

    Immunofluorescence with anti-IDH1 Polyclonal Antibody (PA5-30067) (IDH1_PA5-30067_Antibody_Immunofluorescence.jpg)

    Immunofluorescence with anti-IDH1 Polyclonal Antibody (PA5-30067)

    Immunofluorescence analysis of methanol-fixed A431 cells using IDH1 (PA5-30067) antibody at 1:500 dilution.
  • IDH1 Antibody (PA5-30067) in IHC
    IDH1_PA5-30067_Antibody_Immunohistochemistry.jpg

    Immunohistochemistry with anti-IDH1 Polyclonal Antibody (PA5-30067) (IDH1_PA5-30067_Antibody_Immunohistochemistry.jpg)

    Immunohistochemistry with anti-IDH1 Polyclonal Antibody (PA5-30067)

    Immunohistochemical analysis of paraffin-embedded Breast ca using IDH1 (PA5-30067) antibody at 1:500 dilution.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific