MA5-15132 targets I-kappa-B-alpha in FACS, ICC, IHC (P), IP, and WB applications and shows reactivity with Bovine, Guinea Pig, Human, mouse, Non-human primate, Porcine, and Rat samples.
The MA5-15132 immunogen is gST-I-kappa-B-alpha fusion protein corresponding the amino-terminus of human I-kappa-B-alpha.
MA5-15132 detects I-kappa-B-alpha which has a predicted molecular weight of approximately 39 kDa.
NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).
I-kappa-B-alpha Antibody (MA5-15132) in WB MA5-15132_WesternBlot.jpg
Western Blot with anti-I-kappa-B-alpha Monoclonal Antibody [T.937.7] (MA5-15132)