I-kappa-B-alpha Antibody (T.937.7)

I-kappa-B-alpha Monoclonal Antibody for Western blot, IF, ICC, IHC (P), FACS, IP

>> See 23 other antibodies for NFKBIA (I-kappa-B-alpha; IkappaBalpha; NF-kappa-B inhibitor alpha; ikB-alpha; major histocompatibility complex enhancer-binding protein MAD3; nuclear factor of kappa light chain gene enhancer in B-cells)
Synonyms:
I-kappa-B-alpha; IkappaBalpha; NF-kappa-B inhibitor alpha; ikB-alpha; major histocompatibility complex enhancer-binding protein MAD3; nuclear factor of kappa light chain gene enhancer in B-cells
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: T.937.7
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt), Bovine (Bv), Guinea Pig (GP), Non-human primate (Nhp), Porcine (Po)
Immunogen: GST-I?Ba fusion protein corresponding the amino-terminus of human I?Ba
Ordering Information
Pierce I-kappa-B-alpha Antibody (T.937.7)
Product #
MA5-15132
Size
100 ul
Price
$250.00
Purchase
Add I-kappa-B-alpha Antibody (T.937.7) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:1000
Immunofluorescence (IF) 1:125
Immunocytochemistry (ICC) 1:125
Immunohistochemistry (Paraffin) (IHC (P)) 1:50
Flow Cytometry (FACS) 1:800
Immunoprecipitation (IP) 1:50
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Purification: Affinity chromatography
Storage Buffer: 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol
Preservative: <0.02% sodium azide
Storage Conditions: -20°C
Product Specific Information
It is not recommended to aliquot this antibody.
General Information
NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).
Product Images
  • I-kappa-B-alpha Antibody (MA5-15132) in WB
    MA5-15132_WesternBlot.jpg

    Western Blot with anti-I-kappa-B-alpha Monoclonal Antibody [T.937.7] (MA5-15132) (MA5-15132_WesternBlot.jpg)

    Western Blot with anti-I-kappa-B-alpha Monoclonal Antibody [T.937.7] (MA5-15132)

  • I-kappa-B-alpha Antibody (MA5-15132) in IF
    MA5-15132_Immunofluorescence.jpg

    Immunofluorescence with anti-I-kappa-B-alpha Monoclonal Antibody [T.937.7] (MA5-15132) (MA5-15132_Immunofluorescence.jpg)

    Immunofluorescence with anti-I-kappa-B-alpha Monoclonal Antibody [T.937.7] (MA5-15132)

    Immunofluorescent analysis of I-kappa-B-alpha in HeLa cells using an I-kappa-B-alpha monoclonal antibody (Product # MA5-15132) (red). DNA is labeled using a fluorescent blue dye.
  • I-kappa-B-alpha Antibody (MA5-15132) in IHC
    MA5-15132_Immunohistochemistry_2.jpg

    Immunohistochemistry with anti-I-kappa-B-alpha Monoclonal Antibody [T.937.7] (MA5-15132) (MA5-15132_Immunohistochemistry_2.jpg)

    Immunohistochemistry with anti-I-kappa-B-alpha Monoclonal Antibody [T.937.7] (MA5-15132)

    Immunohistochemical analysis of I-kappa-B-alpha in paraffin-embedded human lung carcinoma using a I-kappa-B-alpha monoclonal antibody (Product # MA5-15132).
  • I-kappa-B-alpha Antibody (MA5-15132) in FACS
    MA5-15132_FlowCytometry.jpg

    Flow Cytometry with anti-I-kappa-B-alpha Monoclonal Antibody [T.937.7] (MA5-15132) (MA5-15132_FlowCytometry.jpg)

    Flow Cytometry with anti-I-kappa-B-alpha Monoclonal Antibody [T.937.7] (MA5-15132)

    Flow cytometric analysis of I-kappa-B-alpha in HeLa cells using a I-kappa-B-alpha monoclonal antibody (Product # MA5-15132) (blue) compared to a nonspecifc negative control antibody (red).
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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