I-kappa-B-alpha Antibody (T.937.7)

I-kappa-B-alpha Monoclonal Antibody for Western blot, IF, ICC, IHC (P), FACS, IP

>> See 21 other antibodies for NFKBIA (I-kappa-B-alpha; IkappaBalpha; NF-kappa-B inhibitor alpha; ikB-alpha; major histocompatibility complex enhancer-binding protein MAD3; nuclear factor of kappa light chain gene enhancer in B-cells)
Synonyms:
I-kappa-B-alpha; IkappaBalpha; NF-kappa-B inhibitor alpha; ikB-alpha; major histocompatibility complex enhancer-binding protein MAD3; nuclear factor of kappa light chain gene enhancer in B-cells
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: T.937.7
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt), Bovine (Bv), Guinea Pig (GP), Non-human primate (Nhp), Porcine (Po)
Immunogen: GST-I-kappa-B-alpha fusion protein corresponding the amino-terminus of human I-kappa-B-alpha.
Ordering Information
Pierce I-kappa-B-alpha Antibody (T.937.7)
Product #
MA5-15132
Size
100 ul
Price
$250.00
Purchase
Add I-kappa-B-alpha Antibody (T.937.7) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:1000
Immunofluorescence (IF) 1:125
Immunocytochemistry (ICC) 1:125
Immunohistochemistry (Paraffin) (IHC (P)) 1:50
Flow Cytometry (FACS) 1:800
Immunoprecipitation (IP) 1:50
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Purification: Affinity chromatography
Storage Buffer: 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol
Preservative: <0.02% sodium azide
Storage Conditions: -20°C
Product Specific Information
MA5-15132 targets I-kappa-B-alpha in FACS, ICC, IHC (P), IP, and WB applications and shows reactivity with Bovine, Guinea Pig, Human, mouse, Non-human primate, Porcine, and Rat samples.

The MA5-15132 immunogen is gST-I-kappa-B-alpha fusion protein corresponding the amino-terminus of human I-kappa-B-alpha.

MA5-15132 detects I-kappa-B-alpha which has a predicted molecular weight of approximately 39 kDa.
General Information
NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).
Product Images
  • I-kappa-B-alpha Antibody (MA5-15132) in WB
    MA5-15132_WesternBlot.jpg

    Western Blot with anti-I-kappa-B-alpha Monoclonal Antibody [T.937.7] (MA5-15132) (MA5-15132_WesternBlot.jpg)

    Western Blot with anti-I-kappa-B-alpha Monoclonal Antibody [T.937.7] (MA5-15132)

  • I-kappa-B-alpha Antibody (MA5-15132) in IF
    MA5-15132_Immunofluorescence.jpg

    Immunofluorescence with anti-I-kappa-B-alpha Monoclonal Antibody [T.937.7] (MA5-15132) (MA5-15132_Immunofluorescence.jpg)

    Immunofluorescence with anti-I-kappa-B-alpha Monoclonal Antibody [T.937.7] (MA5-15132)

    Immunofluorescent analysis of I-kappa-B-alpha in HeLa cells using an I-kappa-B-alpha monoclonal antibody (Product # MA5-15132) (red). DNA is labeled using a fluorescent blue dye.
  • I-kappa-B-alpha Antibody (MA5-15132) in IHC
    MA5-15132_Immunohistochemistry_2.jpg

    Immunohistochemistry with anti-I-kappa-B-alpha Monoclonal Antibody [T.937.7] (MA5-15132) (MA5-15132_Immunohistochemistry_2.jpg)

    Immunohistochemistry with anti-I-kappa-B-alpha Monoclonal Antibody [T.937.7] (MA5-15132)

    Immunohistochemical analysis of I-kappa-B-alpha in paraffin-embedded human lung carcinoma using a I-kappa-B-alpha monoclonal antibody (Product # MA5-15132).
  • I-kappa-B-alpha Antibody (MA5-15132) in FACS
    MA5-15132_FlowCytometry.jpg

    Flow Cytometry with anti-I-kappa-B-alpha Monoclonal Antibody [T.937.7] (MA5-15132) (MA5-15132_FlowCytometry.jpg)

    Flow Cytometry with anti-I-kappa-B-alpha Monoclonal Antibody [T.937.7] (MA5-15132)

    Flow cytometric analysis of I-kappa-B-alpha in HeLa cells using a I-kappa-B-alpha monoclonal antibody (Product # MA5-15132) (blue) compared to a nonspecifc negative control antibody (red).
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific