Human IgM (myeloma), Whole Molecule Control

Human IgM (myeloma), Whole Molecule Control for IF, ICC, IHC, BLOCK, Ctrl

Negative Control, Isotype Control, Immunoglobulin Control, Control
Host / Isotype: Human / IgM
Class: Control
Type: Control
Tested Species Reactivity: Not Applicable (N/A)
Ordering Information
Pierce Human IgM (myeloma), Whole Molecule Control
Product #
2 mg
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Tested Applications Dilution *
Immunofluorescence (IF) Assay Dependent
Immunocytochemistry (ICC) Assay Dependent
Immunohistochemistry (IHC) Assay Dependent
Blocking Assay (BLOCK) Assay Dependent
Control (Ctrl)
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 4.7mg/ml
Purification: purified
Storage Buffer: PBS, pH 7.6
Preservative: no preservative
Storage Conditions: 4° C
Product Specific Information
Purity: The precipitin lines against goat anti-human whole serum are the same as those against goat anti-human IgM, Fc5-mu fragment specific, when assayed by immunoelectrophoresis at an antigen concentration equivalent to 20 mg/ml. No reaction was observed against goat anti-human IgG, Fc fragment specific, goat anti-human IgA, alpha chain specific, or goat anti-human alpha-2-macroglobulin.

This antibody is recommended for use in blocking assays, immunohistochemical, immunocytochemical and immunofluorescent applications.

Storage: After opening, dilute only enough antibody for a single day’s use.
General Information
Serum IgM exists as a pentamer in mammals, predominates in primary immune responses to most antigens, is the most efficient complement fixing immunoglobulin and comprises approximately 10% of normal human serum Ig content. IgM is also expressed on the plasma mem- brane of the B lymphocytes as a monomer. It is the B cell antigen receptor and the H chains each contain an additional hydrophobic domain for anchoring in the membrane. Monomers of serum IgM are bound together by disulfide bonds and a joining (J) chain. Immunoglobulins are useful as qualitative or quantitative protein standards and as blocking agents in certain immunodetection methods. 31146 is a highly-purified IgM immunoglobulin from the serum of a non-immunized animal, primarily used as an experimental control.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific