Part of Thermo Fisher Scientific

Heat Shock Protein 70 Monoclonal Antibody (5A5)

Heat Shock Protein 70 Antibody for GS, IF, IHC (F), IP, Western blot

Synonyms:
Hsp70
Entrez Gene ID:
(Human) 3303, (Murine) 193740
UniProt ID:
P08107
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: 5A5
Species Reactivity: Human (Hu) Murine (Ms) Flatworm (Fw) Amphibian (Am) Fish (Fs) Drosophila (Dm) Yeast (Ys) Avian (Av)
Immunogen: Human recombinant HSP70 over expressed in E. coli.
Ordering Information
Pierce Heat Shock Protein 70 Monoclonal Antibody (5A5)
Product Number Pkg. Size Price Purchase
MA3-007 250 µl $354.00
Add Heat Shock Protein 70 Monoclonal Antibody (5A5) to your cart.


Storage: -20º C, Avoid Freeze/Thaw Cycles
Form: 250 µl of ascites fluid diluted in PBS containing 0.05% sodium azide.


Applications Dilution *
Western Blot (WB) 1:1000
Immunofluorescence (IF) 1:100
Immunohistochemistry (Frozen) (IHC (F)) Assay dependent
Gel Shift (GS) Assay dependent
Immunoprecipitation (IP) Assay dependent
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
MA3-007 detects several members of the heat shock protein 70 kDa (HSP70) gene family including HSP70, HSC70, GRP78, and following heat shock, HSP72 from yeast, Drosophila, fish, mouse, avian, amphibian and human samples.

MA3-007 has been successfully used in Western blot, immunofluorescence, gel shift, immunohistochemistry (frozen), and immunoprecipitation procedures. By Western blot, this antibody detects proteins from ~70 kDa to ~78 kDa representing different members of the HSP70 family. 2-dimensional gel electrophoresis is required to resolve the heat induced form of these proteins from their constitutively expressed counterparts. Immunofluorescence staining of HSP70 in heat shocked HeLa cells with MA3-007 results in cytoplasmic staining.

The MA3-007 antigen is recombinant human HSP70 over-expressed in E. coli. Epitope mapping with a panel of HSP70 deletion mutants suggests that the epitope recognized is located between amino acids 122-264 of human HSP70, a region that has been shown to be involved in ATP binding. This is the first monoclonal antibody reported to react with: 1) the ATP binding region of HSP70, 2) an epitope in the amino terminus of HSP70.
General Information
Heat shock proteins (HSP) are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs including HSP70, HSP90 and HSP100. The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein that is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or HSP75, a 75 kDa protein that is found within the mitochondria.
PubMed References:
Blocking Assay
  Species / Dilution Summary
  Hu / Not Cited
 MA3-007 was used in blocking/activating experiment to investigate the role of Hsp70 in modulation of stress kinase activity
J Biol Chem. 1997 Jul 18;272(29):18033-7.
"Hsp70 prevents activation of stress kinases. A novel pathway of cellular thermotolerance."
Author(s): Gabai VL, Meriin AB, Mosser DD, Caron AW, Rits S, Shifrin VI, Sherman MY
Number of Citations: 36
(See PubMed article )
Flow Cytometry
  Species / Dilution Summary
  Hu / 5 ug/1*10^6 cells
 MA3-007 was used in flow cytometry and western blot to identify the extracelluar part of human Hsp70 and its role in the induction of an NK-regulated immune response
Cell Stress Chaperones. 1998 Mar;3(1):6-11.
"Definition of extracellular localized epitopes of Hsp70 involved in an NK immune response."
Author(s): Botzler C, Li G, Issels RD, Multhoff G
Number of Citations: 15
(See PubMed article )
  Hu / 1:100-1:200
 MA3-007 was used in flow cytometry and immunocytochemistry to investigate the protective roles of heat shock proteins during complement-mediated lysis
Int Immunol. 2001 Aug;13(8):983-91.
"Contribution of heat shock proteins to cell protection from complement-mediated lysis."
Author(s): Fishelson Z, Hochman I, Greene LE, Eisenberg E
Number of Citations: 5
(See PubMed article )
Gel Shift
  Species / Dilution Summary
  Ys / Not Cited
 MA3-007 was used in EMSA to investigate the regulatory roles of yeast heat shock transcription factor.
Mol Biol Cell. 2000 May;11(5):1739-51.
"Complex regulation of the yeast heat shock transcription factor."
Author(s): Bonner JJ, Carlson T, Fackenthal DL, Paddock D, Storey K, Lea K
Number of Citations: 7
(See PubMed article )
Immunocytochemistry
  Species / Dilution Summary
  Hu / 1:100-1:200
 MA3-007 was used in flow cytometry and immunocytochemistry to investigate the protective roles of heat shock proteins during complement-mediated lysis
Int Immunol. 2001 Aug;13(8):983-91.
"Contribution of heat shock proteins to cell protection from complement-mediated lysis."
Author(s): Fishelson Z, Hochman I, Greene LE, Eisenberg E
Number of Citations: 5
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  Dm / 1:100
 MA3-007 was used in immunohistochemistry to study the intrinsic toxicity of PABPN1 in a Drosophila model of oculopharyngeal muscular dystrophy
EMBO J. 2006 May 17;25(10):2253-62.
"A Drosophila model of oculopharyngeal muscular dystrophy reveals intrinsic toxicity of PABPN1."
Author(s): Chartier A, Benoit B, Simonelig M
Number of Citations: 1
(See PubMed article )
  Dm / 1:500
 MA3-007 was used in immunohistochemistry to investigate the effect of autophagy in the pathogenesis of dentatorubral-pallidoluysian Atrophy
Cell Death Differ. 2010 Oct;17(10):1577-87.
"Neurodegeneration by polyglutamine Atrophin is not rescued by induction of autophagy."
Author(s): Nisoli I, Chauvin JP, Napoletano F, Calamita P, Zanin V, Fanto M, Charroux B
Number of Citations: 1
(See PubMed article )
  Ms / 1:100
 MA3-007 was used in immunohistochemistry to identify highly abundant heat shock proteins and chaperone proteins in mature mouse egg proteome.
Reprod Biol Endocrinol. 2003 Feb 14;1():27.
"Oolemmal proteomics--identification of highly abundant heat shock proteins and molecular chaperones in the mature mouse egg and their localization on the plasma membrane."
Author(s): Calvert ME, Digilio LC, Herr JC, Coonrod SA
Number of Citations: 11
(See PubMed article )
Immunoprecipitation
  Species / Dilution Summary
  Hu / 1:100
 MA3-007 was used in immunoprecipitation to investigate the evolutionary conservation of the DNA replication initiators between Gram-negative bacteria plasmids RepA and eukaryotes Orc4p.
Proc Natl Acad Sci U S A. 2001 Apr 24;98(9):4938-43.
"Similarities between the DNA replication initiators of Gram-negative bacteria plasmids (RepA) and eukaryotes (Orc4p)/archaea (Cdc6p)."
Author(s): Giraldo R, Diaz-Orejas R
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
 MA3-007 was used in immunoprecipitation and western blot to understand the mechanism of HETE signalling pathway in carcinoma cells
J Lipid Res. 1998 Feb;39(2):237-44.
"Identification of subunits of the 650 kDa 12(S)-HETE binding complex in carcinoma cells."
Author(s): Herbertsson H, Kühme T, Evertsson U, Wigren J, Hammarström S
Number of Citations: 1
(See PubMed article )
Western Blot
  Species / Dilution Summary
  Am / Not Cited
 MA3-007 was used in western blot to study the role of Scythe and Reaper in Hsp70 chaperone function.
EMBO J. 2001 Mar 1;20(5):1033-41.
"Reversible inhibition of Hsp70 chaperone function by Scythe and Reaper."
Author(s): Thress K, Song J, Morimoto RI, Kornbluth S
Number of Citations: 1
(See PubMed article )
  Bv / 1:10,000-1:15,000
 MA3-007 was used in western blot to study how hsc70 reduces the stress response in Xenopus oocytes
J Biol Chem. 1994 Jun 3;269(22):15718-23.
"hsc70 moderates the heat shock (stress) response in Xenopus laevis oocytes and binds to denatured protein inducers."
Author(s): Mifflin LC, Cohen RE
Number of Citations: 3
(See PubMed article )
  Dm / 1:5,000
Hm / 1:5,000
 MA3-007 was used in western blot to identify the important protein components in the DENV receptor complex in C6/36 cells
Am J Trop Med Hyg. 2007 Aug;77(2):283-90.
"Evidence that the 45-kD glycoprotein, part of a putative dengue virus receptor complex in the mosquito cell line C6/36, is a heat-shock related protein."
Author(s): Salas-Benito J, Reyes-Del Valle J, Salas-Benito M, Ceballos-Olvera I, Mosso C, del Angel RM
Number of Citations: 1
(See PubMed article )
  Hu / 1:2,000
 MA3-007 was used in flow cytometry and western blot to identify the extracelluar part of human Hsp70 and its role in the induction of an NK-regulated immune response
Cell Stress Chaperones. 1998 Mar;3(1):6-11.
"Definition of extracellular localized epitopes of Hsp70 involved in an NK immune response."
Author(s): Botzler C, Li G, Issels RD, Multhoff G
Number of Citations: 15
(See PubMed article )
  Hu / 1:50
 MA3-007 was used in western blot to demonstrate that heat shock protein 70 (hsp70) could be a major target molecule of the humoral response in patients with pulmonary cryptococcosis
Clin Exp Immunol. 1999 Mar;115(3):485-90.
"Heat shock protein 70 (hsp70) as a major target of the antibody response in patients with pulmonary cryptococcosis."
Author(s): Kakeya H, Udono H, Maesaki S, Sasaki E, Kawamura S, Hossain MA, Yamamoto Y, Sawai T, Fukuda M, Mitsutake K, Miyazaki Y, Tomono K, Tashiro T, Nakayama E, Kohno S
Number of Citations: 7
(See PubMed article )
  Mc / Not Cited
 MA3-007 was used in western blot to investigate the effects of cadmium exposure on expression of heat shock proteins (HSPs) in eastern oysters
J Exp Biol. 2008 Feb;211(Pt 4):577-86.
"Effects of cadmium on cellular protein and glutathione synthesis and expression of stress proteins in eastern oysters, Crassostrea virginica Gmelin."
Author(s): Ivanina AV, Cherkasov AS, Sokolova IM
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
 MA3-007 was used in western blot to evaluate the vaccine antigen candidates for Schistosoma mansoni
Infect Immun. 1993 Jan;61(1):146-54.
"Candidate vaccine antigens identified by antibodies from mice vaccinated with 15- or 50-kilorad-irradiated cercariae of Schistosoma mansoni."
Author(s): Richter D, Harn DA
Number of Citations: 2
(See PubMed article )
  Ms / Not Cited
 MA3-007 was used in immunoprecipitation and western blot to understand the mechanism of HETE signalling pathway in carcinoma cells
J Lipid Res. 1998 Feb;39(2):237-44.
"Identification of subunits of the 650 kDa 12(S)-HETE binding complex in carcinoma cells."
Author(s): Herbertsson H, Kühme T, Evertsson U, Wigren J, Hammarström S
Number of Citations: 1
(See PubMed article )
  Ms / 1:250
 MA3-007 was used in western blot to demonstrate that heat shock protein 70 (hsp70) could be a major target molecule of the humoral response in patients with pulmonary cryptococcosis
Clin Exp Immunol. 1999 Mar;115(3):485-90.
"Heat shock protein 70 (hsp70) as a major target of the antibody response in patients with pulmonary cryptococcosis."
Author(s): Kakeya H, Udono H, Maesaki S, Sasaki E, Kawamura S, Hossain MA, Yamamoto Y, Sawai T, Fukuda M, Mitsutake K, Miyazaki Y, Tomono K, Tashiro T, Nakayama E, Kohno S
Number of Citations: 7
(See PubMed article )
  Ms / Not Cited
 MA3-007 was used in western blot to investigate the changes of endoplasmic reticulum chaperone complexes, redox state, and impaired protein disulfide reductase activity in misfolding alpha1-antitrypsin transgenic mice
J Neurophysiol. 2005 Nov;94(5):3563-72.
"Heat shock-mediated thermoprotection of larval locomotion compromised by ubiquitous overexpression of Hsp70 in Drosophila melanogaster."
Author(s): Klose MK, Chu D, Xiao C, Seroude L, Robertson RM
Number of Citations: 1
(See PubMed article )
  Rb / 1:1,000
 MA3-007 was used in western blot to investigate the effect of ischemic pretreatment for HSP72 expression and neural damage in rabbit
J Thorac Cardiovasc Surg. 2000 Sep;120(3):513-9.
"Effect of ischemic pretreatment on heat shock protein 72, neurologic outcome, and histopathologic outcome in a rabbit model of spinal cord ischemia."
Author(s): de Haan P, Vanicky I, Jacobs MJ, Bakker O, Lips J, Meylaerts SA, Kalkman CJ
Number of Citations: 1
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)